Effects of Nitrogen Deposition and Ectomycorrhizal Fungi on Root Architecture and Rhizosphere Soil Enzyme Activities of Larix olgensis Seedlings

doi:10.7525/j.issn.1673-5102.2021.01.018

Abstract

Abstract:

In order to explore the responses of root morphology and rhizosphere soil enzyme activities of one-year-old Larix olgensis seedlings to different simulated nitrogen deposition and ectomycorrhizal fungi， the morphology of roots with diameter of 0-0.5 mm（including the total root length， surface area， volume and tips） and soil enzyme activities（including β-1，4-glucosidase（BG）， L-leucine aminopeptidase（LAP）， β-1，4-N-acetylglucosaminidase（NAG）， acid phosphatase（ACP） and alkaline phosphatase（ALP）） were measured respectively. The different inoculated seedlings of L.olgensis were selected， including the not noculated（-M） and inoculated with 8 ectomycorrhizal fungi（+M） respectively. Four nitrogen deposition treatments were set， including no nitrogen（0N， 0 kg·N·hm^-1·yr^-1）， low nitrogen（LN， 15 kg·N·hm^-1·yr^-1）， middle nitrogen（MN， 30 kg·N·hm^-1·yr^-1） and high nitrogen（HN， 60 kg·N·hm^-1·yr^-1）. The results indicated that： ①The nitrogen deposition concentration had a negative effect on the total root length， surface area， volume， tips with a diameter 0-0.5 mm of L.olgensis seedlings under +M and -M treatments. The root morphology indexes of -M was higher than that of +M treatment under 0N， LN， and MN treatments， respectively. ②With the increasing of nitrogen deposition， the activities of BG， LAP， ACP， ALP in the rhizosphere soils showed a tendency of first rising and then decreasing under the two inoculation treatments， but the activities of NAG showed a decreasing tendency. ③The morphology of roots with diameter of 0-0.5 mm was significantly negatively correlated with the activities of BG in the rhizosphere soils（P<0.05）， under the +M and -M treatments. There was significantly positive correlation between the activities of NAG and root morphology（P<0.05）， except the number of the tips. The effect of nitrogen deposition on root morphology of L.olgensis seedlings was weakened by ectomycorrhizal fungi inoculation treatment. However， the activation of soil enzyme activity by +M was higher than that of -M treatment under low nitrogen deposition.

Key words: Larix olgensis, nitrogen deposition, ectomycorrhizal fungi, root morphology, soil enzyme activities

CLC Number:

S723.1

Ting-Yan LIU, Long-Fei HAO, Xu-Fu WANG, Hai-Xia YAN, Shu-Lan BAI. Effects of Nitrogen Deposition and Ectomycorrhizal Fungi on Root Architecture and Rhizosphere Soil Enzyme Activities of Larix olgensis Seedlings[J]. Bulletin of Botanical Research, 2021, 41(1): 145-151.

Figures/Tables 3

Table 1

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处理 Treatments		总根长 Root length(cm)	总表面积 Root surface area(cm2)	总体积 Root volume(cm³)	根尖数 Tips
-M	0N	70.53±3.54a	7.38±0.24a	0.07±0.00a	222.00±5.20a
	LN	57.23±2.88b	6.68±0.42ab	0.07±0.00a	103.67±3.67c
	MN	46.92±1.86c	5.64±0.60c	0.05±0.00b	94.00±3.00c
	HN	29.41±0.56d	3.48±0.02d	0.03±0.00c	70.67±1.86c
+M	0N	59.05±2.86b	5.91±0.09bc	0.05±0.00b	125.00±13.86b
	LN	52.63±4.98bc	5.59±0.07c	0.05±0.00b	88.00±0.58c
	MN	25.61±0.50d	2.86±0.10d	0.03±0.00c	46.67±2.03d
	HN	29.70±0.99d	3.32±0.09d	0.03±0.00c	48.00±2.31d
M treatment		***	***	***	***
N treatment		***	***	***	***
M*N interaction		**	**	**	***

处理Treatments		BG	LAP	NAG	ACP	ALP
-M	0N	0.71±0.08f	15.15±0.90cd	3.48±0.04cd	21.28±1.53b	53.20±5.35a
	LN	1.71±0.09e	16.99±2.04cd	3.96±0.56bc	22.56±2.19b	57.32±2.38a
	MN	4.86±0.52c	19.27±1.02bc	3.93±0.07bc	25.10±1.03b	63.07±3.62a
	HN	3.96±0.17d	12.70±1.71d	2.29±0.24e	23.54±0.99b	37.15±0.07bc
+M	0N	1.97±0.11e	14.19±0.97cd	6.08±0.67a	22.20±0.11b	25.73±1.77d
	LN	3.68±0.08d	31.13±2.39a	4.79±0.04b	24.18±0.87b	58.96±6.34a
	MN	12.53±0.43a	23.33±2.36b	4.39±0.08bc	29.51±2.21a	42.73±0.58b
	HN	7.46±0.38b	11.93±1.22d	2.90±0.19de	22.49±0.35b	28.84±1.26cd
M treatment		***	**	***	NS	***
N treatment		***	***	***	**	***
M*N interaction		***	**	*	NS	**

处理Treatments		BG	LAP	NAG	ACP	ALP
-M	总根长Root length	-0.740^**	0.346	0.618^*	-0.187	0.591^*
	总表面积Root surface area	-0.620^*	0.467	0.726^**	-0.065	0.694^*
	根系总体积Root volume	-0.638^*	0.425	0.709^**	-0.150	0.706^*
	根尖数Tips	-0.747^**	0.005	0.235	-0.352	0.223
+M	总根长Root length	-0.885^**	0.197	0.755^**	-0.435	0.151
	总表面积Root surface area	-0.924^**	0.209	0.716^**	-0.480	0.138
	根系总体积Root volume	-0.851^**	0.398	0.624^*	-0.338	0.329
	根尖数Tips	-0.820^**	0.016	0.874^**	-0.420	-0.091
总根长Root length		-0.787**	0.106	0.436	-0.369	0.427^*
总表面积Root surface area		-0.791**	0.086	0.350	-0.347	0.516^**
根系总体积Root volume		-0.770**	0.141	0.255	-0.323	0.615^**
根尖数Tips		-0.706**	-0.129	0.176	-0.410*	0.271

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