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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (1): 138-144.doi: 10.7525/j.issn.1673-5102.2021.01.017

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Growth Responses of Pinus sylvestris var. mongolica Seedlings under Simulated Nitrogen Deposition and Different Inoculation of Ectomycorrhizal Fungi Treatments

Xu-Fu WANG1, Long-Fei HAO1, Jia-Xin HAO1, Wen-Ying HAO1, Hui-Ga BAO2, Shu-Lan BAI1()   

  1. 1.College of Forestry,Inner Mongolia Agricultural University,Hohhot 010019
    2.Wuhai City Wetland Administration,Wuhai 016000
  • Received:2019-11-25 Online:2021-01-20 Published:2021-01-05
  • Contact: Shu-Lan BAI E-mail:baishulan2004@163.com
  • About author:WANG Xu-Fu(1992—),male,master candidates,engaged in mycorrhizal biotechnology research.
  • Supported by:
    Postdoctoral Science Foundation Project of China(2018M643778XB);Young Teachers Research Fund Project of Forestry college in Inner Mongolia Agricultural University(203170004);Student Science and Technology Innovation Project of Inner Mongolia Agricultural University(KJCX201910)


The growth responses of Pinus sylvestris var. mongolica seedlings in pot culture conditions were discussed under different inoculation treatments and simulated nitrogen deposition. The different inoculation treatments were performed after 2-month seedling, which included a strain inoculation of Suillus bovinus(SB)and8 strain mixed inoculation(HJ), including Suillus luteusS.grevilleaTricholoma fulvumB.grisellusS.bovinusLeucocortinarius bulbiger Rhizopogon luteolus and Pisolithus tinctorius. The simulated nitrogen deposition treatments were performed for six months after mycorrhizal colonization, which included on nitrogen(CKN), 1-time nitrogen concentration(CN) and 2-time nitrogen concentration(2CN) treatments. The seedling growth index, root morphology and mycorrhizal colonization rate were measured after the 5-month interaction treatments. The results showed that: ①With the increase of nitrogen concentration, the mycorrhizal infection rate showed a downward trend under SBand HJ treatments. The mycorrhizal infection rate of SB inoculation was affected significantly by nitrogen deposition treatments. ②The seedlings aboveground and underground biomass were accumulated significantly after inoculation treatments, which were the best effect under mixed inoculation treatment. The aboveground biomass in the same inoculation treatments were inhibited by the increase of nitrogen concentration. The seedlings ground diameter and above-ground biomass were affected significantly by interaction of inoculation and nitrogen concentration, but the seedlings height and underground biomass had no effects. ③Compared with CK and SB treatments, the seedlings average root diameter, total root length and total surface area of CKN and CN treatments were promoted significantly under HJ treatment. With the increase of nitrogen content, the seedlings average root diameter, total root length and total surface area were promoted significantly under CK and SB treatments, but the morphological indexes were inhibited under HJ treatment. The seedling root morphology were affected significantly by the inoculation, nitrogen concentration, and the interaction treatments.

Key words: nitrogen deposition, Pinus sylvestris var. mongolica, single inoculation, mixed inoculation, root morphology

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