Growth Responses of Pinus sylvestris var. mongolica Seedlings under Simulated Nitrogen Deposition and Different Inoculation of Ectomycorrhizal Fungi Treatments

doi:10.7525/j.issn.1673-5102.2021.01.017

Abstract

Abstract:

The growth responses of Pinus sylvestris var. mongolica seedlings in pot culture conditions were discussed under different inoculation treatments and simulated nitrogen deposition. The different inoculation treatments were performed after 2-month seedling， which included a strain inoculation of Suillus bovinus（SB）and8 strain mixed inoculation（HJ）， including Suillus luteus， S.grevillea， Tricholoma fulvum， B.grisellus， S.bovinus， Leucocortinarius bulbiger， Rhizopogon luteolus and Pisolithus tinctorius. The simulated nitrogen deposition treatments were performed for six months after mycorrhizal colonization， which included on nitrogen（CKN）， 1-time nitrogen concentration（CN） and 2-time nitrogen concentration（2CN） treatments. The seedling growth index， root morphology and mycorrhizal colonization rate were measured after the 5-month interaction treatments. The results showed that： ①With the increase of nitrogen concentration， the mycorrhizal infection rate showed a downward trend under SBand HJ treatments. The mycorrhizal infection rate of SB inoculation was affected significantly by nitrogen deposition treatments. ②The seedlings aboveground and underground biomass were accumulated significantly after inoculation treatments， which were the best effect under mixed inoculation treatment. The aboveground biomass in the same inoculation treatments were inhibited by the increase of nitrogen concentration. The seedlings ground diameter and above-ground biomass were affected significantly by interaction of inoculation and nitrogen concentration， but the seedlings height and underground biomass had no effects. ③Compared with CK and SB treatments， the seedlings average root diameter， total root length and total surface area of CKN and CN treatments were promoted significantly under HJ treatment. With the increase of nitrogen content， the seedlings average root diameter， total root length and total surface area were promoted significantly under CK and SB treatments， but the morphological indexes were inhibited under HJ treatment. The seedling root morphology were affected significantly by the inoculation， nitrogen concentration， and the interaction treatments.

Key words: nitrogen deposition, Pinus sylvestris var. mongolica, single inoculation, mixed inoculation, root morphology

CLC Number:

S718.81

Xu-Fu WANG, Long-Fei HAO, Jia-Xin HAO, Wen-Ying HAO, Hui-Ga BAO, Shu-Lan BAI. Growth Responses of Pinus sylvestris var. mongolica Seedlings under Simulated Nitrogen Deposition and Different Inoculation of Ectomycorrhizal Fungi Treatments[J]. Bulletin of Botanical Research, 2021, 41(1): 138-144.

Figures/Tables 3

Fig.1

Table 1

Table 2

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接种 Inoculation	氮浓度 Nitrogen concentration	苗高 Seedling height (mm)	地径 Ground diameter (mm)	地上生物量 Aboveground biomass (g)	地下生物量 Underground biomass (g)
CK	CKN	52.527±1.232Aa	0.570±0.035Bb	0.009±0.001Cb	0.007±0.000Ca
	CN	53.397±1.814Aa	0.700±0.000Ba	0.017±0.001Ca	0.008±0.001Ca
	2CN	51.083±0.483Ba	0.770±0.012Aa	0.016±0.001Ca	0.010±0.002Ca
SB	CKN	51.620±3.333Aa	0.750±0.084ABa	0.047±0.002Ba	0.020±0.001Ba
	CN	52.567±2.169Aa	0.690±0.029Ba	0.025±0.002Bb	0.020±0.005Ba
	2CN	55.737±0.507Aa	0.823±0.033Aa	0.030±0.001Bb	0.025±0.001Ba
HJ	CKN	58.123±2.584Aa	0.827±0.067Aa	0.067±0.002Aa	0.039±0.006Aa
	CN	56.413±1.137Aa	0.860±0.040Aa	0.057±0.003Ab	0.039±0.001Aa
	2CN	57.357±1.575Aa	0.760±0.000Aa	0.056±0.001Ab	0.052±0.002Aa
ECFM		**	**	***	***
N		NS	NS	***	*
FM×N		NS	*	***	NS

接种 Inoculation	氮浓度 Nitrogen concentration	平均直径 Average diameter (mm)	总根长 Total root length (cm)	总表面积 Total surface area (cm²)	总分叉数 Total bifurcation number
CK	CKN	0.372±0.010Bb	79.366±11.107Bb	9.273±1.546Bc	214.000±77.942Ba
	CN	0.367±0.004Bb	111.724±22.247Cab	12.873±2.590Cb	304.333±21.341Aa
	2CN	0.414±0.005Aa	139.166±13.632Ba	17.185±0.647Ba	242.000±24.826Aa
SB	CKN	0.364±0.001Bb	127.379±20.887Bb	14.855±1.882Bb	173.667±25.115Bb
	CN	0.396±0.004Aa	170.950±20.872Ba	21.360±2.791Ba	333.000±8.660Aa
	2CN	0.392±0.011Ba	196.641±21.362Aa	24.630±2.854Aa	255.667±38.394Aab
HJ	CKN	0.409±0.004Aa	226.221±55.761Aab	27.948±7.880Aab	813.000±17.898Aa
	CN	0.407±0.013Aa	292.103±32.688Aa	34.810±2.654Aa	376.667±60.911Ab
	2CN	0.388±0.003Bb	154.268±6.520Bb	19.592±1.318Bb	175.667±4.910Ac
ECFM		***	***	***	***
N		**	***	***	***
FM×N		***	***	***	***

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