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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (6): 906-912.doi: 10.7525/j.issn.1673-5102.2020.06.013

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Analysis of SHMT Gene Family and Production of PtrSHMT9 Mutant in Populus trichocarpa

Bing LI, Yu-Xiang CHENG()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-06-07 Online:2020-11-20 Published:2020-11-04
  • Contact: Yu-Xiang CHENG E-mail:chengyuxiang@nefu.edu.cn
  • About author:LI Bing(1995—),female,master,mainly engaged in tree secondary growth.
  • Supported by:
    National Natural Science Foundation of China(31570580)


Serine hydroxy methyltransferases(SHMTs) played an important role in the one-carbon(C1) metabolism pathway in plant cells. We identified 9 SHMT genes from the genome of Populus trichocarpa. Populus eFP resource showed that 7 PtrSHMTs were expressed in multiple tissues, among which PtrSHMT9 was the most highly expressed gene in xylem. By analysis of RT-PCR and Aspwood, PtrSHMT9 had a high level in secondary wall thickening of stem wood tissue, suggested its involvement in poplar wood formation. Using Cas9/gRNA technique, PtrSHMT9-edited mutants were prepared and 4 different ptrshmt9 knockouts were obtained. Our results provided basic information and genetic material for exploring the function of SHMT and PtrSHMT9 in trees.

Key words: Populus trichocarpa, SHMT, ptrshmt9mutant, Cas9/gRNA

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