Comparison of Reproductive Biology Including Morphology of Reproductive Organs,Viability of Pollens,Flower Visitors,and Flavonols Accumulation Between Fertile and Sterile Cultivars in Bougainvillea

doi:10.7525/j.issn.1673-5102.2018.06.009

Abstract

Abstract: Plants in Bougainvillea are characterized with high sterility. In this study, morphology of reproductive organs, viability of pollens, flower visitors, and flavonols accumulation were compared between two sterile and four fertile cultivars. The calyx in four fertile cultivars was oval and bigger than that in other sterile cultivars. Pollens were size-consistent in fertile cultivars. Viability of pollens with 11.1 to 46.5 germination rate in fertile cultivars was much higher than that in the sterile. Seven kinds of insect visitors were found in six cultivars, which displayed no discrimination among various cultivars. The results indicated that kaempferol/and quercetin/glycosylated derivatives were the major flavonols accumulating in pollens using the florescence dye, diphenylboric acid 2-aminoethyl ester (DPBA). Florescence strength in tips of pollen tubes was much robuster than that in other parts. Accumulation of flavonols resulted in sharp difference between fertile cultivars and sterile cultivars in the study, which suggested that flavonols especially quercetin might play positive roles in fertility of plants in Bougainvillea.

Key words: Bougainvillea, fertility, pollen, viability, flavonol

CLC Number:

Q949.745.3

XU Su-Xia, HUANG Qing-Yun, LIN Chun-Song, XU Yi-Ding, LI Zi-Yang, LIU Yu-Mei, HUANG Yi-Jin. Comparison of Reproductive Biology Including Morphology of Reproductive Organs,Viability of Pollens,Flower Visitors,and Flavonols Accumulation Between Fertile and Sterile Cultivars in Bougainvillea[J]. Bulletin of Botanical Research, 2018, 38(6): 860-868.

References

1. Mabberley D J. The Plant Book[M]. Cambridge:Cambridge Univ Press,1997:1-706.
2. Balasaraswathi R,Sadasivam S,Ward M,et al. An antiviral protein from Bougainvillea spectabilis roots;purification and characterisation[J]. Phytochemistry,1998,47(8):1561-1565.
3. Schlein Y,Jacobson R L,Muller G C. Sand fly feeding on noxious plants:a potential method for the control of Leishmaniasis[J]. Am J Trop Med Hyg,2001,65(4):300-303.
4. Joseph O,Adebay O,Adesokan A A,et al. Effect of ethanolic extract of Bougainvillea spectabilis leaves on hematological and serum lipid variables in rats[J]. Biochemistry,2005,17(1):45-50.
5. Ali M S,Ibrahin S A,Ahmed F,et al. Color versus bioactivity in the flowers of Bougainvillea spectabilis (Nyctaginaceae)[J]. Nat Prod Res,2005,19(1):1-5.
6. Sharma S C,Srivastava R,Roy R K. Role of Bougainvilleas in mitigation of environmental pollution[J]. Environ Sci Eng,2005,47(2):131-134.
7. Narayanan C R,Joshi D D,Mujumdar A M,et al. Pinitol-a newanti-diabetic compound from the leaves of Bougainvillea spectabilis[J]. Curr Sci,1987,56:139-141.
8. Sarah H B,Jones R B,Bailey C J. Insulin-like effect of pinitol[J]. Brit J Pharm,2000,30:1944-1948.
9. Campbell W W,Haub M D,Fluckey J D,et al. Pinitol supplementation does not affect insulin-mediated glucose metabolism and muscle insulin receptor content and phosphorylation in older humans[J]. Nutr,2004,4:2998-3003.
10. Zadoo S,Khoshoo T N. Cytogenetical studies in Bougainvillea. Ⅰ. A case of interchange heterozygosity[J]. Genetica,1968,39:353-359.
11. Khoshoo T N,Zadoo S. New perspectives in Bougainvillea breeding[J]. Hered,1969,60:357-360.
12. Xu S X,Huang Q Y,Shu Q Y,et al. Reproductive Organography of Bougainvillea spectabilis Willd[J]. Sci Hortic,2009,120(3):399-405.
13. López H A,Galetto L. Flower Structure and Reproductive Biology of Bougainvillea stipitata(Nyctaginaceae)[J]. Plant Biology,2002,4(04):508-514.
14. Coe E H,McCormick S M,Modena S A. White pollen in maize[J]. Hered,1981,72:318-320.
15. Mo Y Y,Nagel C,Taylor L P. Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen[J]. Proc Natl Acad Sci USA,1992,89:7213-7217.
16. Taylor L P,Grotewold E. Flavonoids as developmental regulators[J]. Curr Opin Plant Biol,2005,8:317-323.
17. Lewis D R,Ramirez M V,Miller N D,et al. Auxin and ethylene induce flavonol accumulation through distinct transcriptional networks[J]. Plant Physiol,2011,156:144-164.
18. Sheahan J J,Rechnitz G A. Differential visualization of transparent testa mutants in Arabidopsis thaliana[J]. Anal Chem,1993,65:961-963.
19. Peer W A,Brown D E,Tague B W,et al. Flavonoid accumulation patterns of transparent testa mutants of Arabidopsis[J]. Plant Physiol,2001,126:536-548.
20. Warkins J M,hechler P J,Muday G K. Ethylene-induced flavonol accumulation in guard cells suppresses reactive oxygen cultivars and moderates stomatal aperture[J]. Plant Physiol,2014,164:1707-1717.
21. Hertog M G L,Hollman P C H,Venema D P. Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits[J]. Agric Food Chem,1992a,40:1591-1598.
22. Yang R Z,Wei X L,Gao F F,et al. Simultaneous analysis of anthocyanins and flavonols in petals of lotus(Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry[J]. Chromatography A,2009,1216:106-112.
23. Mahajan M,Ahuja P S,Yadav S K. Post-Transcriptional Silencing of Flavonol Synthase mRNA in Tobacco Leads to Fruits with Arrested Seed Set[J]. PLoS ONE,2011,6(12):e28315.
24. Pollak P E,Vogt T,Mo Y,et al. Chalcone synthase and flavonol accumulation in stigmas and anthers of Petunia hybrida[J]. Plant Physiol,1993,102:925-932.
25. Ylstra B,Touraev A,Benito Moreno R M,et al. Flavonols stimulate development,germination and tube growth of tobacco pollen[J]. Plant Physiol,1992,100:902-907.

[1]	Dan WANG, Zhongshuai ZHANG, Qingyin ZENG, Xuemin HAN. Fruiting and Seed Characteristics of Abies in Northwest Yunnan [J]. Bulletin of Botanical Research, 2023, 43(5): 647-656.
[2]	Shujun ZHNAG, Xinxin YANG, Jian LUO. Micromorphological Characteristics of Pollen and Its Taxonomic Significance of Saussurea in Sedgera Mountains of Tibet [J]. Bulletin of Botanical Research, 2023, 43(5): 741-755.
[3]	Qinghua YANG, Binjie GE, Dasheng ZHANG. Observation on the Formation and Composition Analysis of Blue Pollen of Caryopterisincana [J]. Bulletin of Botanical Research, 2023, 43(5): 794-800.
[4]	Yali ZHANG, Naiying YANG, Yao SONG, Xiangpeng LI, Weizhen GUO, Youming CAI. Pollen Morphological Characters of 20 Species from Camellia Sect. Theopsis and Sect. Eriandria [J]. Bulletin of Botanical Research, 2023, 43(3): 470-480.
[5]	Jianwei YANG, Zongyan LI, Yao FENG, Shuxian REN, Menglu HU, Songpu YE. Reproductive Biological Characteristics of Dendrobium chrysanthum [J]. Bulletin of Botanical Research, 2023, 43(1): 150-160.
[6]	Ying WANG, Yujie ZHANG, Zihui JIAO, Xueli LI, Kai WANG, Xiaowei DA, Kun SUN, Hui ZHANG. Preliminary Study on Cross Fertility in ‘Kushui’ Rose [J]. Bulletin of Botanical Research, 2022, 42(3): 403-411.
[7]	Min LU, Xi-Lin LI, Xiong LU. SEM Sample Preparation of Observation Pollen Grains Morphology in Pollinium [J]. Bulletin of Botanical Research, 2021, 41(5): 836-840.
[8]	Hong-Chi LI, Tian-Yu WU, Li GONG, Jian LUO. Floral Phenotypes and Pollen Morphological Characteristics of 13 Species from Calanthe in Tibet [J]. Bulletin of Botanical Research, 2021, 41(4): 547-556.
[9]	Chen YANG, Zhi-Xiang CHEN, Xue-Ying YAO, He-Shan WANG, Qi-Zhi WANG. Pollen Morphology and Systematic Analysis of Fifteen Sanicula Species from China [J]. Bulletin of Botanical Research, 2020, 40(6): 805-812.
[10]	Yun-Fei XU, Qin-Song LIU, Wen-Juan XU, Jun-Feng TANG, Ting-Fa DONG, Bao-Zhen YANG, Xiao XU. Differences in Population Structure and Dynamic Characteristics of Davidia involucrata Baill. Between High and Low Latitude Regions [J]. Bulletin of Botanical Research, 2020, 40(6): 855-866.
[11]	SUN Yi, DING Su-Qin, SHI Yi-Min, TANG Dong-Qin. Pollen Morphology of 15 Cultivars of Freesia hybrida [J]. Bulletin of Botanical Research, 2019, 39(1): 17-26.
[12]	YU Ting-Ting, NI Xiu-Zhen, GAO Li-Hong, HAN Guo-Jun, ZHU Chang-Fu, SHENG Yan-Min. Advances in Study of Dihydroflavonol 4-Reductase(DFR) Genes of Higher Plants [J]. Bulletin of Botanical Research, 2018, 38(4): 632-640.
[13]	ZHU Li-Kui, TANG Liang, ZHAO Bei-Bei, LU Zhao-Geng, WANG Li. Morphological and Structural Changes during Male Cones Development in Cunninghamia lanceolata [J]. Bulletin of Botanical Research, 2018, 38(3): 349-356.
[14]	DU Hui-Cong, ZHANG Ying, TIAN Min, WANG Cai-Xia. Study on The Seed Biological Characteristics of Chimonanthus praecox of Zhejiang Province [J]. Bulletin of Botanical Research, 2018, 38(1): 7-13.
[15]	CHEN Xiang-Xiang, LU Lu, FAN Li-Jie, WANG Qian-Ying, YIN Zeng-Fang, SHEN Ya-Mei. Distant Hybridization Barriers of Magnolia sinostellata in the Pollination Process [J]. Bulletin of Botanical Research, 2017, 37(6): 816-824.