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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (5): 669-674.doi: 10.7525/j.issn.1673-5102.2018.05.005

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Effects of Exogenous NO on Seed Germination and Physiological Metabolism in Catharanthus roseus Seedling under NaCl Stress

ZHAO Xiao-Ju1,2, ZHANG Li-Xia1, MAN Xiu-Ling2   

  1. 1. Bioengineering Institute, Daqing Normal University, Daqing 163712;
    2. College of Forestry, Northeast Forestry University, Harbin 150040
  • Received:2018-04-08 Online:2018-09-15 Published:2018-08-31
  • Supported by:
    This study was financially supported by Natural Science Foundation of Heilongjiang province(QC2015042)

Abstract: Under 50 mmol·L-1 NaCl stress condition, we studied the relaxation effects of sodium nitroprusside(SNP, an exogenous nitric oxide donor) at the contents of 0.05, 0.1, 0.5, 1.0 and 2.0 mmol·L-1 on germination and physiological metabolism in Catharanthus roseus seedlings. The results showed that NaCl stress inhibited the germination of C.roseus and SNP could alleviate its stress effect. At the 0.1 mmol·L-1 of SNP the seed germination, germination and vitality index, and contents of proline in leaves were increased significantly, while malondialdehyde(MDA) content in leaves were decreased dramatically. Nitrate reductase(NR) activities in 0.1-0.5 mmol·L-1 SNP groups reached to higher levels compared to that of control group. However, more than 0.5 mmol·L-1 SNP enhanced the stress effect of NaCl. Therefore, the 0.1 mmol·L-1 was the most appropriate content for SNP to alleviate NaCl stress. In conclusion, appropriate contents of exogenous NO could alleviate NaCl stress on C.roseus seeds and seedlings effectively.

Key words: Nitric oxide, salt stress, Catharanthus roseus, seed germination, nitrate reductase

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