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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 633-640.doi: 10.7525/j.issn.1673-5102.2021.04.019

• Research report • Previous Articles    

Regulation of NO in the Phenolics Metabolism of Catharanthus roseus under Salt Stress

Xiao-Ju ZHAO1, Yi-Ting ZHANG1, Jia LIU2, Yang LIU2, Zhong-Hua TANG2()   

  1. 1.Bioengineering Institute,Daqing Normal University,Daqing 163712
    2.College of Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040
  • Received:2020-12-15 Online:2021-07-20 Published:2021-03-24
  • Contact: Zhong-Hua TANG E-mail:tangzh@nefu.edu.cn
  • About author:ZHAO Xiao-Ju(1978—),female,associate professor,major in plant physiology and ecology research.
  • Supported by:
    Scientific Research Fund of Daqing Normal University(19ZR07)


In order to uncover the response of phenolic compounds of the Catharanthus roseus under salt stress regulated by exogenous nitric oxide(NO), the ingredient and content of phenolic metabolites in roots, stems, leaves, flowers of the C. roseus were detected using Liquid Chromatography-mass spectrometry(LC-MS) technology after being treated with different concentrations of Sodium Nitroprusside(SNP) under salt stress respectively. The results showed that 5 kinds of C6C1 phenolic compounds including vanillic acid, p-Hydroxybenzoic acid, Syringic acid, Protocatechuic acids and Gallic acid, and 5 kinds of C6C3 phenolic compounds including trans-p-Hydroxycinnamic acid, Chlorogenic acid, Ferulic acid, Cinnamic acid and p-Coumaric acid and 8 kinds of C6C3C6(flavonoids) including Genistein, Petunidin, Naringenin, Quercetin-3-O-rhamnoside, Myricitrin, Quercetin, Kaempferol and Rutin and L- phenylalanine were identified respectively. Among them, quercetin, chlorogenic acid, protocatechuic acids were all in roots, stems, leaves and flowers. The responsive accumulation of the phenolic compounds in different tissues of C. roseus were significantly different under different concentrations of SNP treatment respectively. The small molecular phenolic acids including C6C1and C6C3 were mainly accumulated in roots and stems and C3C6C3 phenolic compounds were enriched mainly in surface leaves and flowers respectively. There were more L-phenylalanine in stem and leaf than in root and flower, and the relative content of L-phenylalanine in stem significantly increased under salt stress, but it showed a downward trend with the increase of NO concentration. Exogenous NO affected the accumulation and change of phenolic compounds in C. roseus under salt stress, and the roots and stems were more sensitive to the response. From the perspective of species and relative content, the stems and leaves were more suitable for the detection of phenolic compounds than others.

Key words: slat stress, nitric oxide, Catharanthus roseus, metabolome, phenolic compounds

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