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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (2): 247-253.doi: 10.7525/j.issn.1673-5102.2018.02.012

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Analysis of BpBEE1 Gene Promoter in Betula platyphylla

XU Si-Jia1, YAN Bin1, DONG Jing-Xiang2, WU Dan-Yang1, LI Hui-Yu1   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    2. Northeast Forest University, Harbin 150040
  • Received:2017-07-18 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    National Key R&D Plan(2017YFD0600603)

Abstract: Brassinolide Enhanced Expression(BEE) genes belong to bHLH transcription factors family, and were involved in Brassinolide signal pathway. In this study, the promoter of BpBEE1 gene was cloned, and regulatory elements in the promoter regions were analyzed using the online program PLACE. The results showed that the BEE1 promoter sequence contained various hormone responses elements. The results showed that the GUS activity was increased in the veins and roots, and the activity of promoter was enhanced after MeJA, SA, BL and ABA treatment, especially MeJA, SA and BL treatment. We propose that BpBEE1 is involved in hormone response, and play a role in the process of plant growth and development.

Key words: Betula platyphylla, BpBEE promoter, Genetic transformation, honmones

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