Abstract: The process and mechanism of leaf protoplast formation after incubation to cell wall digesting solution was investigated. The acid material in the digesting solution provided the acid environment for leaves and led to the occurrence of acid degradation of cell wall, the separation between cell wall with protoplast and the escape of protoplast from cell wall into solution. The cytoplasm membrane inside the digesting solution was degraded by the acid environment and this accounted for the escape of cell nucleus and organelles from protoplast. In digesting solution, the recombination of organelles surrounding nuclear and subsequent formation of new protoplast with unanimous shape took place. These results showed that plant cell wall and cytoplasm membrane is the coat system of plant cell. The acid degradation of plant cell coat system makes recombination of cell organ and production of new protoplast possible.

Key words: leaf, acid degradation of cell wall, protoplast