Abstract: We used the “solid-liquid-liquid-solid” culture method to establish suspension cell line and embryonic suspension cell line from seeds, cotyledons and hypocotyls of Populus hybrids［(Populus pseudo-simonii×P.nigra ‘Zhenyin3#’)×P.×beijingensis］. Seed callus induction rates of different genotypes were significantly different, while cotyledon callus and hypocotyl callus induction rates of different genotypes had no significantly different, and the packed cell volume among different genotypes had significantly different. Double filtration with cytoscreeners after standing and layering process could well enrich uncombined embryonic suspension cell, and the number of uncombined embryonic cells among different genotypes had no significantly different. Only in the media without NH⁺₄, we could obtain embryonic suspension cell lines which were originated from seeds and cotyledon, seed and cotyledon round calli regenerated from differentiation medium including CPPU, and 20 genotypes of the regenerated plants from seed round calli and other 20 genotypes of the regenerated plants from cotyledon round calli.

Key words: Populus, multiple genotypes, suspension cell line, round callus, somatic embryogenesis