Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Schisandra chinensis and the Effects on Anti-Oxidative Stress in Cells

doi:10.7525/j.issn.1673-5102.2023.04.016

Abstract

Abstract:

The polysaccharides of Schisandra chinensis were extracted by ultrasonic-assisted cellulase enzymolysis from the dried fruit of S. chinensis as raw material， and the extraction process and anti-oxidative stress activity of S. chinensis polysaccharides were studied. Using polysaccharide yield as index， single factor experiment and Box-Behnken response surface test were used to investigate the effects of enzyme concentration， ultrasonic power， extraction time and solid-liquid ratio on polysaccharide yield of S. chinensis， and the ultrasonic-assisted enzymatic extraction process was optimized. A lipopolysaccharide（LPS） induced oxidative stress damage model of HepG2 cells was established， and the levels of malondialdehyde（MDA）， superoxide dismutase（SOD） and reactive oxygen species（ROS） were used as indicators， and the anti-oxidative stress ability of S. chinensis polysaccharides was investigated. The results showed that the optimal extraction conditions as followed： the enzyme concentration was 1 420 U·g^-1， the ultrasonic power was 500 W， the extraction time was 46 min， the solid-liquid ratio was 1 g∶32 mL. Under these conditions， the yield of polysaccharides was 22.25%. Moreover， the extracted S. chinensis polysaccharides might improve the SOD level in the LPS-induced HepG2 cells， but reduce the MDA accumulation and ROS fluorescence intensity in the cells， and effectively relieve the LPS-induced oxidative stress in HepG2 cells. The extraction conditions of polysaccharides from S. chinensis were optimized， and it was found that the extracted polysaccharides had good antioxidant stress ability.

Key words: Schisandra chinensis, polysaccharide, response surface method, oxidative stress

CLC Number:

Q539

Hanlin ZHU, Heng ZHAO, Bowen ZHAI, Maoyu ZHANG, Yujie FU. Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Schisandra chinensis and the Effects on Anti-Oxidative Stress in Cells[J]. Bulletin of Botanical Research, 2023, 43(4): 631-640.

Figures/Tables 6

Table 1

Fig.1

Table 2

Table 3

Fig.2

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编码水平 Coding levels	因素Factor
编码水平 Coding levels	加酶量（A） Enzyme concentration/ （U·g^-1）	超声功率（B） Ultrasonic power/W	提取时间（C） Extraction time/min	料液比（D） Solid to liquid ratio
-1	600	390	20	1 g∶20 mL
0	1 200	455	40	1 g∶30 mL
1	1 800	520	60	1 g∶40 mL

序号 No.	因素A Factor A /（U·g^-1）	因素B Factor B /W	因素C Factor C /min	因素D Factor D	多糖得率 Yield of SCPs /%	序号 No.	因素A Factor A /（U·g^-1）	因素B Factor B /W	因素C Factor C /min	因素D Factor D	多糖得率 Yield of SCPs /%
1	1 200	455	60	1 g∶40 mL	18.77	16	1 200	455	60	1 g∶20 mL	20.43
2	1 200	390	40	1 g∶40 mL	13.97	17	1 200	390	60	1 g∶30 mL	14.23
3	1 200	455	40	1 g∶30 mL	20.57	18	1 800	455	40	1 g∶40 mL	18.67
4	600	520	40	1 g∶30 mL	13.87	19	1 200	455	40	1 g∶30 mL	20.95
5	1 800	455	40	1 g∶20 mL	17.21	20	1 200	455	20	1 g∶20 mL	13.25
6	600	455	60	1 g∶30 mL	13.47	21	1 200	455	20	1 g∶40 mL	13.67
7	1 200	520	60	1 g∶30 mL	19.71	22	600	455	40	1 g∶20 mL	13.76
8	1 200	455	40	1 g∶30 mL	21.73	23	1 800	455	20	1 g∶30 mL	15.37
9	1 800	520	40	1 g∶30 mL	19.64	24	1 800	455	60	1 g∶30 mL	19.55
10	600	390	40	1 g∶30 mL	9.53	25	1 800	390	40	1 g∶30 mL	15.42
11	1 200	390	40	1 g∶20 mL	15.43	26	1 200	520	20	1 g∶30 mL	14.69
12	1 200	520	40	1 g∶20 mL	19.59	27	1 200	455	40	1 g∶30 mL	21.09
13	600	455	40	1 g∶40 mL	14.39	28	600	455	20	1 g∶30 mL	9.26
14	1 200	520	40	1 g∶40 mL	19.24	29	1 200	390	20	1 g∶30 mL	10.57
15	1 200	455	40	1 g∶30 mL	22.09

来源 Source	平方和 Sum of Squares	自由度 df	均方 Mean square	F	P
模型Model	379.850 0	14	27.130 0	39.420 0	<0.000 1**
A	83.110 0	1	83.110 0	120.740 0	<0.000 1**
B	63.430 0	1	63.430 0	92.150 0	<0.000 1**
C	71.790 0	1	71.790 0	104.290 0	<0.000 1**
D	0.076 8	1	0.076 8	0.111 6	0.743 3
A×B	0.003 6	1	0.003 6	0.005 2	0.943 4
A×C	0.000 2	1	0.000 2	0.000 3	0.985 8
A×D	0.172 2	1	0.172 2	0.250 2	0.624 7
B×C	0.462 4	1	0.462 4	0.671 8	0.426 2
B×D	0.308 0	1	0.308 0	0.447 5	0.514 4
C×D	1.080 0	1	1.080 0	1.570 0	0.230 5
A²	88.600 0	1	88.600 0	128.720 0	<0.000 1**
B²	57.490 0	1	57.490 0	83.520 0	<0.000 1**
C²	72.450 0	1	72.450 0	105.260 0	<0.000 1**
D²	13.000 0	1	13.000 0	18.890 0	0.000 7**
残差Residual	9.640 0	14	0.688 3	—	—
失拟项Lack of Fit	8.130 0	10	0.812 9	2.160 0	0.238 7
纯误差Pure Error	1.510 0	4	0.376 9	—	—
总误差Cor Total	389.490 0	28	—	—	—

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