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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (1): 30-35.doi: 10.7525/j.issn.1673-5102.2023.01.004

• Genetic and Breeding • Previous Articles     Next Articles

Construction of Suppression Expression Vector and Genetic Transformation of BpSPL9 gene from Betula platyphylla

Jinxia DU1, Tingting SHEN3, Haoran WANG3, Yiping LIN3, Huiyu LI3, Lianfei ZHANG2()   

  1. 1.Changchun Polytechnic Modern Agricultural College,Changchun 130118
    2.Anqing national forest authority of Heilongjiang,Harbin 150699
    3.Northeast Forestry University,Harbin 150000
  • Received:2022-05-24 Online:2023-01-20 Published:2022-12-23
  • Contact: Lianfei ZHANG E-mail:zhanlianfei@163.com
  • About author:DU Jinxia(1981—),female,master,mainly engaged in development and breeding of forestry resources.
  • Supported by:
    Research project on teaching reform of vocational education and adult education in Jilin Province in 2021(2021ZCY225);National key research and development projects in the 13th five year plan(2017YFD0600603)


To clarify the function of SPL gene in growth and development of Betula platyphylla,the BpSPL9 gene was cloned, and the expression vector of BpSPL9 was constructed by CREST technology, and the genetic transformation was carried out by Agrobacterium mediated method, and the phenotype of the transgenic lines was observed, respectively. The results showed that the transgenic lines with BpSPL9 suppressed were successfully obtained. The height of transgenic lines was significantly lower than that of non-transgenic lines, with shorter node spacing and smaller leaf area. The contents of 6-BA and IAA in transgenic lines were lower than those of the non-transgenic lines. It is speculated that BpSPL9 gene participated in plant growth and development by affecting the synthesis of auxin and cell division.

Key words: Betula platyphylla, SPL9, CREST, genetic transformation

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