Identification and Analysis of the Potato StCRKs Gene Family and Expression Patterns in Response to Stress Signals

doi:10.7525/j.issn.1673-5102.2022.06.013

Abstract

Abstract:

A total of 18 new StCRKs（cysteine-rich receptor-like kinases， CRKs） family genes were identified in potato genome using online tools such as Pfam. These StCRKs localize on chromosome 1， 2， 11 and 12， and all contain conserved CRK domains. In the promoter regions of these StCRKs genes， there are five plant hormones responsive elements， the circadian control element， biotic and abiotic responsive elements， and seed-specific regulation element. The tissue-specific expression pattern were measured using qRT-PCR method in root， stem， leave， and flower tissues in potato during flowering time. The results showed different StCRKs expressed in different tissues. After salicylic acid analogue BTH and 4 ℃ treatment， it was found that 10 StCRKs genes could be induced by BTH and 13 StCRKs genes were able to respond to the low temperature signal. The results provide clues for further elucidation functions of StCRKs on biotic and abiotic stresses.

Key words: StCRKs, bioinformatics analysis, stress, expression patterns, Potato

CLC Number:

Q939.92

Denggao LI, Rui LIN, Qinghui MU, Na ZHOU, Yanru ZHANG, Wei BAI. Identification and Analysis of the Potato StCRKs Gene Family and Expression Patterns in Response to Stress Signals[J]. Bulletin of Botanical Research, 2022, 42(6): 1033-1043.

Figures/Tables 8

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基因名称 Gene name	序列 Sequence ID	染色体 Locus	基因长度 Genomic /bp	CDS长度CDS /bp	qRT-PCR引物（5′→3′） Primers of qRT-PCR（5′→3′）
StCRK9	Soltu.DM.01G003430.1	Chr1	4 789	1 695	GACGACAAAGGGTACATTGTTC AACAATCAGCGAGAACATAAGC
StCRK10	Soltu.DM.01G003440.1	Chr1	5 831	1 938	AAGTGAGAGCTAGAGGATATGG CCAACAATCGGCGAGAACAT
StCRK11	Soltu.DM.01G003450.1	Chr1	2 555	1 863	TAGTTTCCAATGTGTGGGAGC TAAGTTGGCAGTCGAAGCAG
StCRK12	Soltu.DM.01G003460.1	Chr1	2 252	1 404	ACTTTCAATGCGGGATAGTGG GCGAGTACATCTCGCAAGG
StCRK13	Soltu.DM.01G003470.1	Chr1	2 490	1 389	ACATAAGCACTGTCATTGCTGG ACATGTAACACTCTCGCAACC
StCRK14	Soltu.DM.01G003480.1	Chr1	2 536	1 869	GATACAGTCAGCATAGTTTCCG GCTTGAGTGCACAAAAGTCC
StCRK15	Soltu.DM.01G003490.1	Chr1	6 864	1 965	CAACATTACGGCCATCTATGTC TACTTCTCTCGAGGGTTGTG
StCRK16	Soltu.DM.01G003500.1	Chr1	3 295	1 743	AGAGTACTTAGCACACGGTC GTTGGGATTTCTTGAGTGCAC
StCRK17	Soltu.DM.01G003510.1	Chr1	4 486	1 938	TCGCGAGAGTTTTGCATGTG GAGAACACCATGGAACTGCAT
StCRK18	Soltu.DM.02G019570.1	Chr2	3 380	1 686	GTAGCAGTAAAGAGATTGGCTG TGATGCGAAGTCTAGAATCCTC
StCRK19	Soltu.DM.02G019620.1	Chr2	3 915	2 022	CATGGCAAGGCTATTTACATTG TGGGGTCAATCAAATTTGCAGC
StCRK20	Soltu.DM.02G019650.1	Chr2	3 850	1 938	AACTCAAGGCAGCACAAACAG GCCTCATTTCGTGATGAACC
StCRK21	Soltu.DM.02G019660.1	Chr2	9 799	2 172	TTCAGGCCAAGAGAGACTTC TTTCACTGAAAATTGCCCCTGC
StCRK22	Soltu.DM.02G019670.1	Chr2	5 196	1 677	AGGGCCAGCATTCTATATGC TGCCTCATTTCGTGATGAACC
StCRK23	Soltu.DM.02G019690.1	Chr2	7 277	2 067	TGAGTTTTGCTTGGTTAAGCTGG TGCCATGGTTGGTCTATCAG
StCRK24	Soltu.DM.11G001100.1	Chr11	4 079	1 929	GCACTAAGTTTGCTGGTACC CTTGGTGTTCTTGATTCTGTGC
StCRK25	Soltu.DM.11G001180.1	Chr11	4 053	1 920	TGAAATAATCAGTGGACGGAGG CATGCTTGGCCTAAGATTTGG
StCRK26	Soltu.DM.12G006670.1	Chr12	3 276	1 749	AGGCTATCAAGAAGGTCAAGTC ACGTTGCTTGTTTCTTCGTATC

蛋白名称 Protein name	氨基酸总数量 Amino acid	等电点 Isoelectric point	分子质量 Molecular weight /kDa	消光系数 Extinction coefficients	不稳定系数 Instability index	亲水系数 Hydropathicity
StCRK9	564	7.82	62.26	57 270	31.97	0.002
StCRK10	645	8.56	71.37	68 145	33.58	-0.116
StCRK11	620	8.20	68.97	67 810	35.73	-0.133
StCRK12	467	7.93	51.76	53 455	37.46	-0.332
StCRK13	462	8.93	51.46	52 870	36.36	-0.129
StCRK14	622	8.38	69.16	61 155	31.01	-0.134
StCRK15	654	8.71	72.94	73 200	39.24	-0.154
StCRK16	580	8.50	64.16	69 635	35.85	-0.097
StCRK17	645	7.82	71.43	68 270	33.70	-0.140
StCRK18	561	8.55	62.77	46 730	43.34	-0.094
StCRK19	673	5.95	75.43	67 240	53.34	-0.161
StCRK20	645	6.15	71.76	55 655	42.58	-0.195
StCRK21	723	5.88	80.18	67 045	34.84	-0.252
StCRK22	558	6.44	62.13	44 975	45.53	-0.191
StCRK23	688	5.97	76.55	64 260	40.15	-0.164
StCRK24	642	8.37	71.15	69 635	42.51	-0.191
StCRK25	639	8.79	71.05	76 750	35.97	-0.205
StCRK26	582	7.19	66.20	53 705	45.87	-0.196

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