Fig.1

Schematic diagram of construction for StNPR4 binary expression vector

Fig.2

PCR amplification，enzyme digestion and structure prediction of StNPR4A.PCR amplification of StNPR4 CDS（M.DNA Marker；1.StNPR4 CDSfragments）；B.Enzyme digestion of StNPR4 cloning vector（M.DNA Marker； 1.Double digestion of StNPR4-pMD19-T vector；2.StNPR4-pMD19-T vector）；C.StNPR4 gene structure；D.StNPR4 protein structural domains

Fig.3

Phylogenetic analysis of StNPR4Protein families aligned with potato（Solanum taberosum） StNPR4 proteins include Arabidopsis thaliana，Brasica napus，Capsicum annuun，Solanumn iyeopersicum and Nicotiana tabacum

Fig.4

Expression pattern of StNPR4 in different tissues from S. tuberosum

Fig.5

PCR amplification of StNPR4 promoter and enzyme digestion of the vectorA.PCR amplification of the StNPR4 promoter（M.DNA Marker；1.StPRONPR4 fragments at 1 246 bp；B.Enzyme digestion identification of StNPR4 cloning vector（M.DNA Marker；1.StPRONPR4 fragments at 1 246 bp；2.StproNPR4 -pENTR L16 vector）

Fig.6

Enzyme digestion identification of vectors and PCR identification of transgenic plantsA.Identification of StPRONPR4-StNPR4 pENTR L16 cloning vector by enzymatic digestion；M.DNA Marker；1.StPRONPR4-StNPR4 fragments at 3 021 bp；B.Identification of StPRONPR4-StNPR4 pORE O4 binary expression vector by enzymatic digestion；M.DNA Marker；1.StPRONPR4-StNPR4 fragments at 3 021 bp；C.PCR identification of different StNPR4 transgenic lines；M.DNA Marker；1-5.Different StNPR4 transgenic lines

Fig.7

Functional analysis of StNPR4 transgenic plantsA.StNPR4 expression level in transgenic lines；B.Relative biomass of pathogens after inoculation of transgenic lines with phytophthora infestans；C.Rooting rates of transgenic lines after treatment with 150 mmol·L-1 NaCl；* represents P<0.05，** represents P<0.01，and equal variance t-test was used to analyze each experimental group and the control group at the same time point，the same as below

Fig.8

StNPR4 expression level after BTH treatment