Dynamic Changes of Endogenous Hormones in Rootstocks and Scions within Nurse Seedling Graft in Camellia oleifera Under Wound

doi:10.7525/j.issn.1673-5102.2021.02.011

Abstract

Abstract:

In order to analyze the physiological response to the surface healing of rootstocks and scions， and to provide the theoretical support for the growth mechanism， the dynamic changes of endogenous hormones in post-traumatic stress response in Camellia oleifera A. under wound were studied. The six-year-old Changlin 18 and Changlin 53 trees were used， and the contents of IAA， ABA， TZR， Zeatin， SA， JA were assayed using HPLC-MS at different wound stages， and the changes of endogenous hormone in different period and relationship between varieties were analyzed. The results showed that the content of IAA， SA and JA decreased with the increasing time of wound in scions of Changlin 18， and the content of TZR and Zeatin increased first and decreased later which they were respectively reached the highest in S10 and S0. The content of ABA rose continually and reached the peak in S10. In scions of Changlin 53， the contents of IAA and JA would decrease after peak； the TZR， Zeatin， SA were gradually increased after minimum. The content of ABA was gradually decreased in S0 after maximum. The contents of endogenous hormones were higher in Changlin18 than in Changlin53 in stem of rootstock between varieties. The endogenous hormones were decreased in Z0 and increased in Z10 in varieties except the contents of ABA and SA in Changlin53 increased first and then decreased. In stem and root of rootstocks， the contents of endogenous hormones were higher in Changlin 18 than in Changlin 53 except JA； The contents of IAA and ABA in stem of rootstocks were higher than roots which other endogenous hormones in root of rootstocks were higher than in stem. There was difference in the ratio of hormone in varieties and part of rootstocks. The ratios of IAA/ABA， IAA/TZR， IAA/Zeatin， IAA/JA， ABA/TZR， ABA/Zeatin， ABA/JA in scions and rootstocks were higher in Changlin 53 than in Changlin 18. The ratio of SA/IAA in scions was higher in Changlin 18 than Changlin 53， and it was higher in Changlin 18 than Changlin 53 in ratios of SA/JA， SA/ABA of scions while it was higher in Changlin 18 in stem of rootstocks. The ratio of TZR/SA， TZR/JA in scions and stem of rootstocks were higher in Changlin 18 than Changlin 53. There was the significantly positive correlation in IAA and JA after wound， and the correlation of IAA and SA， SA and JA had difference between varieties， the significantly positive correlation in Changlin 18 and the significantly or significantly negative correlation in Changlin 53. There was the significantly positive correlation among TZR， Zeatin， SA and the significantly negative correlation among TZR， Zeatin， SA. In stem of rootstocks， there was very significantly positive correlation among hormones in Changlin 18， and there was the significantly positive correlation among TZR， IAA and JA， Zeatin and IAA and JA while there was the significantly negative correlation in TZR and SA， Zeatin and SA. There was significantly negative correlation in SA and JA. In stem and root of rootstocks， there was the difference in correlation of SA and other hormones among varieties， and other hormones in Changlin 18 and Changlin 53 had the same significantly positive or negative correlation. To sum up， it was easy to callus formation in surface of wound in Changlin 18， but it was difficult in Changlin 53 because there was much more high-level hormone of stress-resistant so that it would be possible that affects healing after grafting wound 10 min. The differences in ratio and contents of hormones between rootstocks and scions may be affected by the morphological reconstruction of grafted unions and the growth of scions in later stage.

Key words: Camellia oleifera, nurse seedling graft, scion, rootstock, wound, endogenous hormone

CLC Number:

S603.8

Wei LONG, Xiao-Hua YAO, Le-Yan LÜ. Dynamic Changes of Endogenous Hormones in Rootstocks and Scions within Nurse Seedling Graft in Camellia oleifera Under Wound[J]. Bulletin of Botanical Research, 2021, 41(2): 232-242.

Figures/Tables 8

Fig.1

Fig.2

Fig.3

Table 1

Table 2

Table 3

Fig.4

Fig.5

References 38

1	庄瑞林.中国油茶：2版［M］.北京：中国林业出版社，2008：1-117.
	Zhuang R L.China oil tea：2^nd ed［M］.Beijing：China Forestry Press，2008：1-117.
2	庄瑞林.我国油茶良种选育工作的历史回顾与展望［J］.林业科技开发，2010，24（6）：1-5.
	Zhuang R L.Review on the improved variety selection of oil Camellia［J］.China Forestry Science and Technology，2010，24（6）：1-5.
3	国家林业和草原局政府网.国家林业局关于印发《全国油茶主推品种目录》的通知（林场发〔2017〕64号）［EB/OL］.（2017-06-30）［2017-06-27］..
4	康乐，杨水平，姚小华，等.不同品种油茶嫁接苗根系生长动态研究［J］.林业科学研究，2010，23（3）：467-471.
	Kang L，Yang S P，Yao X H，et al.Study on root growth dynamic characteristics of grafted seedlings of different Camellia oleifera cultivars［J］.Forest Research，2010，23（3）：467-471.
5	龙伟，姚小华，王开良，等.采穗位置对油茶嫁接育苗的影响［J］.江西农业大学学报，2014，36（1）：158-163.
	Long W，Yao X H，Wang K L，et al.Effect of scion position on grafting breeding seedling in Camellia oleifera［J］.Acta Agriculturae Universitatis Jiangxiensis，2014，36（1）：158-163.
6	龙伟，姚小华，王开良，等.油茶穗条芽位对嫁接育苗的影响［J］.林业科技开发，2012，26（6）：62-66.
	Long W，Yao X H，Wang K L，et al.The effect of bud location on grafting plant in Camellia oleifera［J］.China Forestry Science and Technology，2012，26（6）：62-66.
7	龙伟，姚小华，吕乐燕，等.修剪模式对油茶采穗圃穗条性状及矿质元素的影响［J］.西南农业学报，2018，31（7）：1468-1476.
	Long W，Yao X H，Lü L Y，et al.Effects of pruning modes on scion character of Camellia oleifera L.and its content of mineral element in cutting orchard［J］.Southwest China Journal of Agricultural Sciences，2018，31（7）：1468-1476.
8	江泽鹏，王东雪，曾雯珺，等.秋季修剪对油茶良种采穗圃穗芽质量的影响［J］.江西农业学报，2013，25（12）：47-48，56.
	Jiang Z P，Wang D X，Zeng W J，et al.Effect of autumn pruning on panicle-sprout quality of Camellia oleifera in improved variety cutting orchard［J］.Acta Agriculturae Jiangxi，2013，25（12）：47-48，56.
9	罗雄飞，李新生，王哲东，等.超声活化酶解木质素用于酚醛树脂的制备［J］.森林工程，2017，33（3）：39-43.
	Luo X F，Li X S，Wang Z D，et al.Preparation of phenol-formaldehyde resin using enzymatic hydrolysis lignin activated with ultrasonic wave［J］.Forest Engineering，2017，33（3）：39-43.
10	马灼明，李淑君，杨冬梅.酶解木质素的活化及用于酚醛树脂的制备［J］.森林工程，2017，33（2）：64-67.
	Ma Z M，Li S J，Yang D M.Activation of enzymatic hydrolysis lignin and used for preparation of phenol-formaldehyde resin［J］.Forest Engineering，2017，33（2）：64-67.
11	时金金，郭元茹，潘清江.以碱木质素胺盐为模板制备纳米二氧化钛及其光催化性能研究［J］.森林工程，2015，31（3）：54-56，62.
	Shi J J，Guo Y R，Pan Q J.Synthesis of TiO₂ nanoparticles prepared by amine salt of alkali lignin and its photocatalytic activities［J］.Forest Engineering，2015，31（3）：54-56，62.
12	Hudina M，Orazem P，Jakopic J，et al.The phenolic content and its involvement in the graft incompatibility process of various pear rootstocks（Pyrus communis L.）［J］.Journal of Plant Physiology，2014，171（5）：76-84.
13	Cookson S J，Moreno M J C，Hevin C，et al.Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls［J］.Journal of Experimental Botany，2014，65（9）：2473-2481.
14	Koepke T，Dhingra A.Rootstock scion somatogenetic interactions in perennial composite plants［J］.Plant Cell Reports，2013，32（9）：1321-1337.
15	Fernández‐García N，Carvajal M，Olmos E.Graft union formation in tomato plants：peroxidase and catalase involvement［J］.Annals of Botany，2004，93（1）：53-60.
16	冯金玲，陈辉，陈世品，等.油茶芽苗砧嫁接口愈合与内源激素的关系［J］.森林与环境学报，2018，38（1）：27-32.
	Feng J L，Chen H，Chen S P，et al.Relationship between endogenous hormone level and the development of the nurse seedling grafted union in Camellia oleifera［J］.Journal of Forest and Environment，2018，38（1）：27-32.
17	Wang C R，Yang A F，Yin H Y，et al.Influence of water stress on endogenous hormone contents and cell damage of maize seedlings［J］.Journal of Integrative Plant Biology，2008，50（4）：427-434.
18	韩宁林，高继银，黄爱珠.油茶芽苗嫁接技术［J］.林业科技通讯，1981，（10）：7-9.
	Han N L，Gao J Y，Huang A Z.The technology of nurse seed graft in Camellia oleifera［J］.Forest Science and Technology，1981，（10）：7-9.
19	Bi Y L，Zhang J，Song Z H，et al.Arbuscular mycorrhizal fungi alleviate root damage stress induced by simulated coal mining subsidence ground fissures［J］.Science of the Total Environment，2019，652：398-405.
20	Zhang G F，Zhao F，Chen L Q，et al.Jasmonate-mediated wound signalling promotes plant regeneration［J］.Nature Plants，2019，5（5）：491-497.
21	Beloshistov R E，Dreizler K，Galiullina R A，et al.Phytaspase-mediated precursor processing and maturation of the wound hormone systemin［J］.New Phytologist，2018，218（3）：1167-1178.
22	Ikeuchi M，Iwase A，Rymen B，et al.Wounding triggers callus formation via dynamic hormonal and transcriptional changes［J］.Plant Physiology，2017，175（3）：1158-1174.
23	Takahashi F，Hanada K，Kondo T，et al.Hormone-like peptides and small coding genes in plant stress signaling and development［J］.Current Opinion in Plant Biology，2019，51：88-95.
24	Li K，Liang Y Q，Xing L B，et al.Transcriptome analysis reveals multiple hormones，wounding and sugar signaling pathways mediate adventitious root formation in apple rootstock［J］.International Journal of Molecular Sciences，2018，19（8）：2201.
25	Koo A J K，Howe G A.The wound hormone jasmonate［J］.Phytochemistry，2009，70（13-14）：1571-1580.
26	Zhang T，Poudel A N，Jewell J B，et al.Hormone crosstalk in wound stress response：wound-inducible amidohydrolases can simultaneously regulate jasmonate and auxin homeostasis in Arabidopsis thaliana［J］.Journal of Experimental Botany，2016，67（7）：2107-2120.
27	杜维，阮成江，丁健，等.液相色谱—串联质谱法同时测定油茶不同组织中5类内源激素［J］.分析科学学报，2018，34（1）：37-42.
	Du W，Ruan C J，Ding J，et al.Simultaneous determination of five plant hormones in different organs of Camellia oleifera by high performance liquid chromatography-tandem mass spectrometry［J］.Journal of Analytical Science，2018，34（1）：37-42.
28	Pan X Q，Welti R，Wang X M.Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry［J］.Nature Protocols，2010，5（6）：986-992.
29	Poór P，Czékus Z，Tari I，et al.The Multifaceted roles of plant hormone salicylic acid in endoplasmic reticulum stress and unfolded protein response［J］.International Journal of Molecular Sciences，2019，20（23）：5842.
30	张苏芳，孔祥波，王鸿斌，等.植物对昆虫不同防御类型及内在联系［J］.应用昆虫学报，2013，50（5）：1428-1437.
	Zhang S F，Kong X B，Wang H B，et al.Different strategies of plant resistance to insects and their interactions［J］.Chinese Journal of Applied Entomology，2013，50（5）：1428-1437.
31	帅海威，孟永杰，罗晓峰，等.生长素调控种子的休眠与萌发［J］.遗传，2016，38（4）：314-322.
	Shuai H W，Meng Y J，Luo X F，et al.The roles of auxin in seed dormancy and germination［J］.Hereditas（Beijing），2016，38（4）：314-322.
32	刘广超，丁兆军.生长素介导环境信号调控植物的生长发育［J］.植物学报，2018，53（1）：17-26.
	Liu G C，Ding Z J.Auxin regulates plant growth and development by mediating various environmental cues［J］.Chinese Bulletin of Botany，2018，53（1）：17-26.
33	Yuan H W，Zhao L，Chen J J，et al.Identification and expression profiling of the Aux/IAA gene family in Chinese hickory（Carya cathayensis Sarg.）during the grafting process［J］.Plant Physiology and Biochemistry，2018，127：55-63.
34	Yin H，Yan B，Sun J，et al.Graft-union development：a delicate process that involves cell-cell communication between scion and stock for local auxin accumulation［J］.Journal of Experimental Botany，2012，63（11）：4219-4232.
35	杨喜田，陈久美，唐妍，等.侧柏幼苗切根后根系内源激素含量的变化［J］.河南农业大学学报，2011，45（1）：66-70.
	Yang X T，Chen J M，Tang Y，et al.Changes of endogenous phytohormone contents in Platycladus orientalis roots after root-cutting［J］.Journal of Henan Agricultural University，2011，45（1）：66-70.
36	王金祥，严小龙，潘瑞炽.不定根形成与植物激素的关系［J］.植物生理学通讯，2005，41（2）：133-142.
	Wang J X，Yan X L，Pan R C.Relationship between adventitious root formation and plant hormones［J］.Plant Physiology Communications，2005，41（2）：133-142.
37	王庆美，张立明，王振林.甘薯内源激素变化与块根形成膨大的关系［J］.中国农业科学，2005，38（12）：2414-2420.
	Wang Q M，Zhang L M，Wang Z L.Formation and thickening of tuberous roots in relation to the endogenous hormone concentrations in sweetpotato［J］.Scientia Agricultura Sinica，2005，38（12）：2414-2420.
38	Sakakibara H.CYTOKININS：activity，biosynthesis，and translocation［J］.Annual Review of Plant Biology，2006，57：431-449.

品种 Varieties	激素 Hormone	吲哚乙酸 IAA	脱落酸 ABA	反式玉米素 TZR	玉米素 Zeatin	水杨酸 SA	茉莉酸 JA
18号	IAA	1
	ABA	-0.311	1
	TZR	0.700^*	0.339	1
	Zeatin	0.487	-0.300	0.422	1
	SA	0.977^**	-0.426	0.578	0.494	1
	JA	0.943^**	-0.576	0.501	0.571	0.962^**	1
53号	IAA	1
	ABA	-0.763^*	1
	TZR	-0.593	0.098	1
	Zeatin	-0.432	-0.103	0.935^**	1
	SA	-0.787^*	0.409	0.879^**	0.734^*	1
	JA	0.845^**	-0.410	-0.869^**	-0.812^**	-0.889^**	1

品种 Varieties	激素 Hormone	吲哚乙酸IAA	脱落酸 ABA	反式玉米素 TZR	玉米素 Zeatin	水杨酸 SA	茉莉酸 JA
18号	IAA	1
	ABA	0.889^*	1
	TZR	0.922^**	0.981^**	1
	Zeatin	0.928^**	0.921^**	0.934^**	1
	SA	0.914^*	0.980^**	0.999^**	0.934^**	1
	JA	0.999^**	0.889^*	0.927^**	0.937^**	0.919^**	1
53号	IAA	1
	ABA	0.007	1
	TZR	0.856^*	-0.475	1
	Zeatin	0.918^**	-0.212	0.937^**	1
	SA	-0.606	0.548	-0.845^*	-0.839^*	1
	JA	0.731	-0.603	0.973^**	0.900^*	-0.915^*	1

品种 Varieties	激素 Hormone	吲哚乙酸 IAA	脱落酸 ABA	反式玉米素 TZR	玉米素 Zeatin	水杨酸 SA	茉莉酸 JA
18号	IAA	1
	ABA	0.988^**	1
	TZR	-0.965^**	-0.964^**	1
	Zeatin	-0.971^**	-0.995^**	0.959^**	1
	SA	0.979^**	0.995^**	-0.979^**	-0.991^**	1
	JA	-0.975^**	-0.977^**	0.994^**	0.970^**	-0.984^**	1
53号	IAA	1
	ABA	0.985^**	1
	TZR	-0.997^**	-0.984^**	1
	Zeatin	-0.996^**	-0.979^**	0.987^**	1
	SA	-0.980^**	-0.957^**	0.969^**	0.992^**	1
	JA	-0.954^**	-0.906*	0.940^**	0.966^**	0.963^**	1

[1]	Xiaopeng MU, Xiaoyan MOU, Xinliang QI, Jing WANG, Yu YANG, Jiancheng ZHANG, Pengfei WANG. Effects of Different Growth Retardant Treatments on Rooting of Prunus humilis Rhizome Cuttings [J]. Bulletin of Botanical Research, 2025, 45(1): 88-97.
[2]	Yanjun REN, Xiaorui GUO, Zixuan YU, Kexin WU, Yu SUN, Ning CHEN, Qiaomu YOU, Kaixin XING. Characteristics of Temperature Stratification on Germination Physiology and Metabolism of Acanthopanax senticosus Seeds at Different Stages [J]. Bulletin of Botanical Research, 2024, 44(4): 576-589.
[3]	Chunjing JIAO, Mingyue LI, Peng ZHANG. Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica [J]. Bulletin of Botanical Research, 2023, 43(3): 370-378.
[4]	Liben PAN, Xue YAN, Jia LIU, Kexin WU, Yang LIU, Shaochong LIU. Physiological Characteristics of Early Spring Flowering Plants under Northeast Forest [J]. Bulletin of Botanical Research, 2022, 42(4): 657-666.
[5]	Guobin Liu, Ting Liao, Ye Wang, Liqin Guo, Jinzhe Zhao, Yanwu Yao, Jun Cao. Regulation Mechanism of Endogenous Hormones in Adventitious Roots Formation of Platycladus orientalis ‘Beverleyensis’ [J]. Bulletin of Botanical Research, 2022, 42(2): 278-288.
[6]	Si-Han WANG, Tao YANG, Jin-Zhu ZHANG, Jie DONG, Zhi-Ling KOU, Dai-Di CHE. Effects of Changes in Seed Cell Tissue Structure and Endogenous Hormones on Dormancy of Wild Rosa rugosa Fruit during Development [J]. Bulletin of Botanical Research, 2021, 41(3): 387-394.
[7]	Zhi-Yuan LIU, Li ZENG, Xi-Wu DU, PENG-Yong-Zheng, Yi-Wei TAO, Yu-Qing LI, Jun QIN. Flower Bud Differentiation and Endogenous Hormone Changes of Rosa ‘Angela’ [J]. Bulletin of Botanical Research, 2021, 41(1): 37-43.
[8]	Yang-Jing ZHAO, Nan TANG, Dao-Cheng TANG, Jing ZHANG. An Optimized HPLC Procedure for Analyzing Three Endogenous Hormones in Tulip Bulbs [J]. Bulletin of Botanical Research, 2021, 41(1): 130-137.
[9]	DENG Jun-Jie, ABDUL MAJEED BALOCH, HOU Xue-Yue, LI Jia-Zhe, CHA Xin-You, ZHANG Rong-Shu. Effect of Trichoderma harzianum T6 on Rosa chinensis ‘Shi-Jie-Mei’ Growth [J]. Bulletin of Botanical Research, 2020, 40(5): 666-672.
[10]	WANG Ning, DONG Ying-Ying, YUAN Mei-Li, WANG Ya-Nan, ZENG Sheng-Yun. Effect of Warm Water Soaking on Protective Enzyme Activity and Endogenous Hormones Content of Eucommia ulmoides Seed During Different Stages of Germination [J]. Bulletin of Botanical Research, 2020, 40(4): 523-529.
[11]	WANG Yue-Lin, XU Da-Ping, YANG Zeng-Jiang, LIU Xiao-Jin, HONG Zhou, ZHANG Ning-Nan. Effects of Different Cultivation Strategies on Endogenous Hormones Accumulation of Dalbergia odorifera [J]. Bulletin of Botanical Research, 2018, 38(5): 688-696.
[12]	LU Yan-Xi, ZHANG Dong, WANG Li-Feng, WANG Ji-Kun. Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg. [J]. Bulletin of Botanical Research, 2017, 37(6): 953-960.
[13]	YANG Liu, WANG Le, XIE Zhong-Kui, GUO Zhi-Hong, ZHANG Yu-Bao. Influence of Endogenous IAA and GA₃ on in vitro Lily Seedling Growth [J]. Bulletin of Botanical Research, 2016, 36(5): 760-767.
[14]	LIU Ya-Jie；CHANG Ping；TANG Dong-Qin*. Endogenous Hormone Contents of Cut Freesia Flowers during Vase Holding [J]. Bulletin of Botanical Research, 2015, 35(2): 218-224.
[15]	DENG Shao-Li;JIANG Guo-Bin;REN Xian;JIN Hua;MA Jin-Long;ZOU Ji-Xiang. Responses of Endogenous Hormones to Drought Stress in the Apoplast of Populus* [J]. Bulletin of Botanical Research, 2013, 33(6): 690-696.