Cloning,Structure and Function Analysis of MiMYB2 Gene from Macadamia integrifolia

doi:10.7525/j.issn.1673-5102.2020.06.014

Abstract

Abstract:

In order to study the mechanism of R2R3-MYB transcription factor gene family member in growth，development and yield formation in Macadamia integrifolia，the MiMYB2 gene was cloned from the leaves of M.integrifolia “GUIRE1” cultivar by PCR and its structure and function were analyzed by bioinformatics method. The cloned cDNA sequence of MiMYB2（NCBI accession number：MN254976） gene is 1 210 bp in length，has 1002 bp open reading frame（ORF），encoding 333 amino acids. MiMYB2，an unstable hydrophilic protein that has no transmembrane structure，no signal peptide and localizes in the nucleus，has two SANT conserved domains belonging to the R2R3-MYB family. BLAST analysis showed that the MiMYB2 share high amino acid sequence identity with Lotus NnMYB3-like protein. Phylogenetic analysis clustered MiMYB2 and AtMYB17，AtMYB106 and AtMYB16 into S9 subfamily. The transcriptome data were used to analyze the expression patterns of MiMYB2 gene in branch，flower and leave of M.integrifolia “GUIRE1” and “695” cultivar. Results indicated that MiMYB2 has the lowest expression in the flower of “GUIRE1”，and the expression level of “695” is the highest. It is speculated that MiMYB2 is closely related to M.integrifolia growth and development. This study would provide a theoretical reference for clarification the role MiMYB2 in growth，development and yield formation mechanism of M.integrifolia.

Key words: Macadamia integrifolia, MiMYB2, gene cloning, bioinformatics analysis

CLC Number:

S664.9

Wen-Lin WANG, Hai-Sheng CHEN, Shu-Fang ZHENG, Song-Le FAN, Li-Feng WANG, Qiu-Jin TAN, Zhen-Shi QIN, Xi-Yun HUANG, Peng HE, Xiu-hua TANG, Peng XU. Cloning,Structure and Function Analysis of MiMYB2 Gene from Macadamia integrifolia[J]. Bulletin of Botanical Research, 2020, 40(6): 913-922.

Figures/Tables 7

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