Cloning and Expression Analysis of RbohB Gene in Brassica campestris L.

doi:10.7525/j.issn.1673-5102.2017.05.016

Abstract

Abstract: A novel RbohB gene was isolated from Brassica campestris L. Longyou 6 by RACE. The full-length cDNA of RbohB is 2 694 bp, with open reading frame (ORF) 2 541 bp, encoding a 846 amino acid protein. By RT-PCR analysis, transcript level of RbohB was increased in response to cold, high salt and H₂O₂ stress. However, MAPKK inhibitor U0126 pretreatment 12 h before cold, high salt and H₂O₂ stress, compared with the single stress, the expression level of RbohB was significantly decreasing. The results suggested that RbohB played an important role during cold, high salt and H₂O₂ stress in B.campestris L. and U0126 has inhibitory effect to RbohB gene transcription. The activity level of NADPH oxidase was increased in response to H₂O₂ stress. However, U0126 pretreatment 12 h before H₂O₂ stress, compared with the single H₂O₂ stress, NADPH oxidase activity was significantly decreasing. The result showed that MAPKK inhibitor U0126 has inhibitory effect to NADPH oxidase activity.

Key words: Brassica campestris L., RbohB gene, molecular cloning, expression analysis

CLC Number:

S565.4

ZHANG Teng-Guo, LUO Dan-Yu, GUO Yan-Feng, ZHENG Sheng, WANG Juan. Cloning and Expression Analysis of RbohB Gene in Brassica campestris L.[J]. Bulletin of Botanical Research, 2017, 37(5): 751-760.

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