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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (5): 744-750.doi: 10.7525/j.issn.1673-5102.2017.05.015

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Effects of Exogenous GA3 on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling

JIANG Yong1,2, HU Shang-Lian1,2, CAO Ying1,2, LU Xue-Qin1,2, HUANG Yan1,2, XU Gang1,2   

  1. 1. Lab of Plant Cell Engineering, School of life science and Engineering, Southwest University of Science and Technology, Mianyang 621010;
    2. Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province, Mianyang 621010
  • Received:2017-02-21 Online:2017-09-15 Published:2017-09-15
  • Supported by:
    National Natural Science Foundation of China(31400257,31400333);Breeding Program Fund project by the 13th Five-year plan of Sichuan province(2016NYZ0038);Fund of Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan province(12zxsk07,13zxsk01);Graduate innovation fund of Southwest University of Science and Technology(16ycx068)

Abstract: We studied the growth of stem and CesA gene expression from the seedling in Phyllostachys edulis under the different concentration of exogenous GA3(0, 0.1, 0.5, 1 μmol·L-1). Compared with without GA3 treatment, the internode and fiber cells of stem from the seedling elongated significantly, after treated by exogenous GA3. The relative expression of primary wall-related PeCesA2 and PeCesA6 genes were significantly up-regulated, while that of secondary wall-related PeCesA4 and PeCesA4-1 genes were significantly down-regulated. By Fourier transform infrared spectroscopy(FTIR), the absorption intensity of characteristic cellulose peak(1 060, 1 160 and 1 373 cm-1) from the stems enhanced with the increase of GA3 concentration. Signifying the expression of CesA gene could be affected by the exogenous GA3. The expression of PeCesA2 and PeCesA6 genes could be related to the process of fiber cells elongation.

Key words: Phyllostachys edulis seedling, GA3, fiber cell, CesA gene

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