Effects of Exogenous GA3 on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling

doi:10.7525/j.issn.1673-5102.2017.05.015

Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (5): 744-750.doi: 10.7525/j.issn.1673-5102.2017.05.015

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Effects of Exogenous GA₃ on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling

JIANG Yong^1,2, HU Shang-Lian^1,2, CAO Ying^1,2, LU Xue-Qin^1,2, HUANG Yan^1,2, XU Gang^1,2

1. Lab of Plant Cell Engineering, School of life science and Engineering, Southwest University of Science and Technology, Mianyang 621010;
2. Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province, Mianyang 621010

Received:2017-02-21 Online:2017-09-15 Published:2017-09-15
Supported by:
National Natural Science Foundation of China(31400257,31400333);Breeding Program Fund project by the 13th Five-year plan of Sichuan province(2016NYZ0038);Fund of Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan province(12zxsk07,13zxsk01);Graduate innovation fund of Southwest University of Science and Technology(16ycx068)

Abstract

Abstract: We studied the growth of stem and CesA gene expression from the seedling in Phyllostachys edulis under the different concentration of exogenous GA₃(0, 0.1, 0.5, 1 μmol·L^-1). Compared with without GA₃ treatment, the internode and fiber cells of stem from the seedling elongated significantly, after treated by exogenous GA₃. The relative expression of primary wall-related PeCesA2 and PeCesA6 genes were significantly up-regulated, while that of secondary wall-related PeCesA4 and PeCesA4-1 genes were significantly down-regulated. By Fourier transform infrared spectroscopy(FTIR), the absorption intensity of characteristic cellulose peak(1 060, 1 160 and 1 373 cm^-1) from the stems enhanced with the increase of GA₃ concentration. Signifying the expression of CesA gene could be affected by the exogenous GA₃. The expression of PeCesA2 and PeCesA6 genes could be related to the process of fiber cells elongation.

Key words: Phyllostachys edulis seedling, GA₃, fiber cell, CesA gene

CLC Number:

S795.9

JIANG Yong, HU Shang-Lian, CAO Ying, LU Xue-Qin, HUANG Yan, XU Gang. Effects of Exogenous GA₃ on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling[J]. Bulletin of Botanical Research, 2017, 37(5): 744-750.

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Effects of Exogenous GA₃ on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling

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