Cloning and Transient Expression Analysis of Promoter of PnsICE1 from Populus simonii×P.nigra

doi:10.7525/j.issn.1673-5102.2015.01.013

Abstract

Abstract: ICE1 encodes a MYC-like bHLH transcriptional activator, which could specially bind to active domain of CBF3 promoter and induce transcriptal expression of cold-responsive genes downstream of CBF3, play important roles in stress response. A 1 247 bp sequence of PnsICE1 promoter was cloned from DNA of Populus simonii×P.nigra by PCR amplification method. By sequence analysis, it consisted of a typical core promoter region of eukaryotic, beside the basic elements: TATA-box and CAAT-box, and there were some hormone responsive elements and multiple stress-induced elements of ABRE, DOFCOREZM, MYBCORE, MYCCONSENSUSATHSE and W-box. PnsICE1 promoter may associated with adversity stress and play important roles in response to various stresses in P.simonii×P.nigra. To verify the biology function, recombinant vector was designated as pBI121-ICEPro through replacing CaMV35S promoter in pBI121 by cloned PnsICE1 promoter fragment. In the vector, gus reporter gene was driven by PnsICE1 promoter. GUS histochemical assay in Arabidopsis thaliana after transient infection showed that gus expressed in both flower and root.

Key words: Populus simonii×, P.nigra, PnsICE1 promoter, regulatory element, abiotic stress

CLC Number:

WANG Yan-Min1；BAI Hui1；YU Wen-Xi2；XING Ya-Juan1*. Cloning and Transient Expression Analysis of Promoter of PnsICE1 from Populus simonii×P.nigra[J]. Bulletin of Botanical Research, 2015, 35(1): 77-83.

References

1.朱玉贤,李毅.现代分子生物学:第2版［M］.北京:高等教育出版社,2002:71.

2.Benfey P N,Ren L,Chua N H.Combinatorial and synergistic properties of CaMV 35S enhancer subdomains［J］.The EMBO Journal,1990,9(6):1685-1696.

3.张俊莲,王蒂,张金文,等.用绿色荧光蛋白和洋葱表皮细胞检测拟南芥rd29A基因启动子活性的方法［J］.植物生理学通讯,2005,41(6):815-819.

4.Kasuga M,Miura S,Shinozaki K,et al.A combination of the Arabidopsis DREB1A gene and stress inducible rd29A promoter improved drought-and low-temperature stress tolerance in tobacco by gene transfer［J］.Plant Cell Physiology,2004,45(3):346-350.

5.Pellegrineschi A,Reynolds M,Pacheco M,et al.Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions［J］.Genome,2004,47:493-500.

6.陆璇璇.小麦脱水素基因wzy1-2启动子的功能分析［D］.杨凌：西北农林科技大学,2013.

7.石东乔,周奕华,万里红,等.甘蓝型油菜BcNA1基因启动子在转基因烟草中对GUS基因表达的调控［J］.植物生理学报,2001,27(4):313-320.

8.王轩鹏,李振波,童超波.甘蓝型油菜BnRabGDI3基因启动子的克隆和功能验证［J］.中国油料作物学报,2013,35(2):109-116.

9.许兰珍,彭爱红,何永睿.异源韧皮部特异启动子在转基因枳中的表达［J］.园艺学报,2014,41（1）:1-8.

10.Chinnusamy V,Ohta M,Kanrar S,et al.ICE1:a regulator of cold-induced transcriptome and freezing tolerance in Arabiopsis［J］.Genes Development,2003.17（8）:1043-1054.

11.黄家权.抗寒基因的克隆及根癌农杆菌介导的ICE1基因转化柑橘的研究［D］.武汉:华中农业大学,2005.

12.林元震,张志毅,刘纯鑫.甜杨抗冻转录因子ICE1基因的in silico克隆及其分析［J］.分子植物育种,2007.5（3）:424-430.

13.赵玲玲.苹果MdSAMDC2和MdICE1基因的功能鉴定以及抗逆性研究［D］.泰安:山东农业大学,2007.

14.张瑜,向殿军,殷奎德.农杆菌介导的大花蕙兰转ICE1基因［J］.中国农学通报,2008,24（12）:40-43.

15.向殿军,满丽莉,殷奎德.拟南芥ICE1基因转化水稻的进一步研究［J］.生物技术通报,2008（6）:87-90,96.

16.郑银英,崔百明,常明进,等.转拟南芥ICE1基因增强烟草抗寒性的研究［J］.西北植物学报,2009,29（1）:75-79.

17.Zheng L,Liu G F,Meng X N,et al.A Versatile Agrobacterium-Mediated Transient Gene Expression System for Herbaceous Plants and Trees［J］.Biochem Genet,2012,50:761-769.

18.Yamaguchi-Shinozaki K,Shinozaki K.Organization of cisacting regulatory elements in osmotic-and cold-stress-responsive promoters［J］.Trends Plant Sci,2005,10（2）:88-94.

19.Abe H,Yamaguchi-Shinozaki K,Urao T,et al.Role of Arabidopsis MYC and MYB homologs in drought-and abscisic acid-regulated gene expression［J］.The Plant Cell Online,1997,9（10）:1859-1868.

20.Plesch G,Ehrhardt T,Mueller-Roeber B.Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cellspecific gene expression［J］.Plant J,2001,28（4）:455-464.

21.Narusaka Y,Nakashima K,Shinwari Z K,et al.Interaction between two cis-acting elements,ABRE and DRE,in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses［J］.Plant J,2003,34（2）:137-148.

22.Eulgem T,Rushton P J,Robatzek S,et al.The WRKY superfamily of plant transcription factors［J］.Trends Plant Sci,2000,5（5）:199-205.

23.Rushton P J,Somssich I E,Ringler P,et al.WRKY transcription factors［J］.Trends Plant Sci,2010,15（5）:247-258.

24.Puranik S,Sahu P P,Srivastava P S,et al.NAC proteins :regulation and role in stress tolerance［J］.Trends Plant Sci,2012,176（6）:369-381.

25.Nakashima K,Takasaki H,Mizoi J,et al.NAC transcription factors in plant abiotic stress responses［J］.BBAGene Regul Mech,2012,1819（2）:97-103.

26.Puranik S,Bahadur R P,Srivastava P S,et al.Molecular cloning and characterization of a membrane associated NAC family gene,SiNAC from foxtail millet(Setaria italica（L.）P.Beauv.)［J］.Mol Biotechnol,2011,49:138-150.

27.Han Q Q,Zhang J H,Li H X,et al.Identification and expression pattern of one stress-responsive NAC gene from Solanum lycopersicum［J］.Mol Biol Rep,2011,39:1713-1720.

28.Nakashima K,Tran L S P,Van Nguyen D,et al.Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice［J］.Plant J,2007,51（4）:617-630.

29.Fang Y J,You J,Xie K B,et al.Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice［J］.Mol Genet Genomics,2008,280:547-563.

30.Zheng X N,Chen B,Lu G J,et al.Overexpression of a NAC transcription factor enhances rice drought and salt tolerance［J］.Biochem Biophys Res Commun,2009,379（4）:985-989.

31.曹仪植.植物分子生物学［M］.北京:高等教育出版社，2002:226,202-203,252-253.

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