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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (6): 913-920.doi: 10.7525/j.issn.1673-5102.2018.06.015

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Cloning and Expression Pattern Analysis of MUR5 Gene in Fraxinus mandshurica

WANG Heng-Tao, SHAO Wan-Xuan, XU Shu-Hao, ZENG Fan-Suo   

  1. College of Life Sciences, Northeast Forestry University, Harbin 150040
  • Received:2018-05-14 Online:2018-11-15 Published:2018-11-01
  • Supported by:
    University of Northeastern Forestry Undergraduate Innovation Project(201710225302)

Abstract: WE studied the expression pattern of FmMUR5 in different phases and abiotic stress, and revealed the phytohormone-induced expression characteristics. We bioinformatically analyzed the FmMUR5 gene and the encoded protein in Fraxinus mandshurica. The coding region of FmMUR5 is 1 080 bp in length and encodes 359 amino acids. The FmMUR5 protein is a stable, hydrophilic protein in the absence of signal peptide and transmembrane structure. The genetic distance between FmMUR5 and Pinus sylvestris var. mongolica and Hordeum vulgare was relatively close, indicating that they are relatively close genetic relationship. The expression of FmMUR5 gene was the highest in newly formed shoots with tissue specificity, and the expression of FmMUR5 in each part was relatively high in August. Under abiotic stress the induction of hormone signal, the expression level of FmMUR5 gene changed with the time of treatment, but the trend was not the same. FmMUR5 gene expression was highest by treated for 1 h at low temperature (4℃), salt (NaCl) and mannitol three abiotic stresses, 1.5, 1.4 and 6.3 times of the control group, respectively. FmMUR5 was the lowest at 4℃ and 24 h after mannitol treatment, while FmMUR5 was the lowest at 12 h after salt treatment (NaCl). The expression level of FmMUR5 gene was the highest at 1 h after abscisic acid (ABA) and auxin (IAA) treatment and 12 h after gibberellin (GA) treatment, 2.5, 3.8 and 1.1 times of the control group, respectively. The expression level of FmMUR5 was the lowest at 24 h after abscisic acid (ABA) treatment and lowest at 6 hours after treatment with IAA and GA. The results indicate that FmMUR5 is involved in abiotic stress and plant hormone signal induction.

Key words: Fraxinus mandshurica, FmMUR5, bioinformatics analysis, abiotic stress

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