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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (3): 424-432.doi: 10.7525/j.issn.1673-5102.2020.03.014

• Research Report • Previous Articles     Next Articles

Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response

LIU Jia-Li1, HE Ming-Liang2,3, LIU Chen-Xi1, LIAO Xu1, LI Xiu-Feng2,3, GUAN Qing-Jie1   

  1. 1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040;
    2. Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, Harbin 150081;
    3. University of the Chinese Academy of Sciences, Beijing 100049
  • Received:2019-10-01 Online:2020-05-05 Published:2020-05-29
  • Supported by:
    Natural Science Foundation of Heilongjiang Province of China(C2017009);National Key Research and Development Program of China(2016YFC0501203);Fundamental Research Funds for the Central Universities(2572016EAJ6);Heilongjiang Postdoctoral Scientific Research Developmental Fund(LBH-Q15004)

Abstract: C2HC type zinc finger protein was screened, encoded 304 amino acids and named OsZFP6. The expression level of OsZFP6 was increased by abiotic stress, including alkali(NaHCO3) and H2O2 treatment in two-week old rice leaves but decreased in roots. Thus, it was speculated that OsZFP6 was involved in the regulation corresponding reaction pathways of resistance. We fused a green fluorescent protein(GFP) with it and found that OsZFP6 was located in the nucleus. Moreover, rice overexpressed OsZFP6 were more tolerant to NaHCO3 treatments. The overexpressed plants had strong tolerance, and were higher than WT in plant height and fresh weight, but the contents of MDA and H2O2 in vivo were lower than non-transgenic plants, which indicated that OsZFP6 could enhance the resistance of plants by scavenging peroxide in response to NaHCO3 stress. Preliminarily, it was speculated that OsZFP6 gene participated in the regulation of resistance and it may play a vital role in transcription regulation.

Key words: zinc finger protein, abiotic stress, rice, basic salt

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