Abstract: The pollens of Magnolia sinostellata were employed as experimental material to study the effects of different concentrations of sucrose, boron, calcium on pollen vitality of M.sinostellata through single factorial experiment and the orthogonal design. Moreover, the effects of storage condition and storage time on pollen germination were studied. The results showed that: the optimum medium was sucrose 30 g·L^-1+H₃BO₃ 200 mg·L^-1+ CaCl₂ 200 mg·L^-1, in which the rate of germination was 74.56%. Low temperature contributes to the maintaining of the vitality of M.sinostellata. Storage under -80℃ condition, the vitality of pollen dropped in a slow rate. As the increase of storage time at low temperature, the vitality of pollen dried by silicone was higher than the moist pollen.

Key words: Magnolia sinostellata, pollen, germinate, storage