Abstract: Tissue culture of Dioscorea opposita “foshou” was studied with explants such as tubers，leaves，stem segments. The results showed that the media of MS+6-BA1.0 mg·L^-1+NAA0.1 mg·L^-1 was for tubers and the media of MS+6-BA0.5~1.0 mg·L^-1+NAA2.0 mg·L^-1 was for leaves in the dark condition. The tissue culture of stems was conducted in light condition. The optimum media was MS + 6-BA1.0 mg·L^-1 + NAA0.5 mg·L^-1 for the stem without the node. MS+ 6-BA0.5~1.0 mg·L^-1 + NAA0.1 mg·L^-1 was suitable for primary culture of the stem with the node, the optimum propagating media was MS+6-BA0.5 mg·L^-1+ NAA0.1 mg·L^-1, roots were induced on the media of 1/2MS+NAA0.5 mg·L^-1.

Key words: Dioscorea opposita &ldquo, foshou&rdquo, tubers, leaves, stem segments, tissue culture