Abstract: According to the cDNA sequence of transcription factor DREB1A gene in GenBank a pair of primer was designed and synthesized.Using the total RNA of low temperature treated Arabdopsis thaliana seedling as templet,the full length of cDNA of DREB1A gene was amplified through method of RT-PCR.It was cloned into pMD18 T vector.DNA sequencing indicated that the cloned fragment showed 99.8% identity to the sequence of the gene in GenBank.The Two bases change leads to only one amino acid changed.But the changed amino acid is not in the function domain so the cDNA will have the normal physiology function.The plant express vector pBch was digested and ligated with the cloned cDNA fragment and got the DREB1A gene express vector pBDR35s regulated by 35S promoter.

Key words: transcription factor, DREB1A, gene cloning, construction of plant express vector