Abstract: The flavonoids is used molecular marker to study the relation between natural population of Elaeagnus mollis and the habitat. The flavonoid, which is important secondary metabolit that is ubiquitous in plant, was extracted by the methynol and water from the leaves of Elaeagnus mollisand was isolated by polyamide thin-layer chromatography. The band spectrum was statistical and analyzed by hierarchical cluster and PCA analysis. The reasult showed 1)that in E. molls population was stable and was as marker for studying population differentiation of E. molls. 2) Cluster analysis indicated That the flavonoids differentiation was into among 3 populations. The dissimilarity between Xiangning and Yicheng population was small by clustering in 20 value but Pinglu population in 25 value. 3) The share ratio is obviously different among populations. 4) PCA analysis indicated that flavonoids difference among population is the relationship to its environmentail factors.

Key words: Eleaegnus mollis, flavonoid, cluster analysis, PCA analysis