Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (4): 637-646.doi: 10.7525/j.issn.1673-5102.2022.04.013
• Molecular biology • Previous Articles Next Articles
Xueying GUO1, Kailin ZHU2, Xin CHEN2, Xueqin FU1, Dongqin TANG1()
Received:
2021-09-01
Online:
2022-07-20
Published:
2022-07-15
Contact:
Dongqin TANG
E-mail:dqtang@sjtu.edu.cn
About author:
GUO Xueying(1998—),female,pastgraduate,mainly engaged in research on ornamental plants.
Supported by:
CLC Number:
Xueying GUO, Kailin ZHU, Xin CHEN, Xueqin FU, Dongqin TANG. Karyotype and Cluster Analysis of Six Freesia hybrida Cultivars[J]. Bulletin of Botanical Research, 2022, 42(4): 637-646.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.04.013
Table 1
Basic information of 6 cultivars of F. hybrida
编号Code | 品种 Cultivars | 花色 Flower color | 花型 Flower types | 来源 Origin | 亲本 Parents |
---|---|---|---|---|---|
1 | ‘Gold River’ | 黄色 Yellow | 单瓣型 Single petal type | 荷兰 Netherlands | 未知 Unknown |
2 | ‘White River’ | 白色 White | 单瓣型 Single petal type | 荷兰 Netherlands | 未知 Unknown |
3 | ‘Castor’ | 蓝紫色 Blue violet | 单瓣型 Single petal type | 荷兰 Netherlands | 未知 Unknown |
4 | ‘SN Hongtaige’ | 深红色 Dark red | 重瓣型 Double petal type | 中国 China | ‘Red Lion’[ |
5 | ‘Pink Passion’ | 粉红色 Pink | 单瓣型 Single petal type | 荷兰 Netherlands | 未知 Unknown |
6 | ‘Red River’ | 红色 Red | 单瓣型 Single petal type | 荷兰 Netherlands | 未知 Unknown |
Table 2
The karyotype analysis of six cultivars of F. hybrida
品种 Cultivars | 染色体组编号 Chromosome number | 相对长度(L+S=T) Relative length /% | 臂比(L/S) Arm ratio | 着丝粒类型 Centromere type | 染色体编号 Chromosome number | 相对长度(L+S=T) Relative length/% | 臂比(L/S) Arm ratio | 着丝粒类型 Centromere type |
---|---|---|---|---|---|---|---|---|
‘Gold River’ | 1 | 7.71+5.27=12.98 | 1.46 | m | 7 | 4.56+4.16=8.71 | 1.10 | m |
2 | 6.95+3.59=10.54 | 1.94 | m | 8 | 4.92+3.23=8.15 | 1.52 | sm | |
3 | 5.61+4.45=10.06 | 1.26 | sm | 9 | 4.60+3.11=7.71 | 1.48 | m | |
4 | 5.87+4.18=10.05 | 1.40 | m | 10 | 4.21+2.87=7.07 | 1.47 | m | |
5 | 6.35+3.33=9.68 | 1.90 | m | 11 | 3.18+2.97=6.15 | 1.07 | m | |
6 | 5.07+3.82=8.89 | 1.33 | sm | |||||
‘White River’ | 1 | 7.82+4.26=12.09 | 1.84 | sm | 7 | 5.26+3.67=8.93 | 1.43 | m |
2 | 6.96+4.58=11.54 | 1.52 | m | 8 | 5.54+3.14=8.68 | 1.77 | m | |
3 | 6.02+4.65=10.67 | 1.29 | m | 9 | 3.94+3.31=7.25 | 1.19 | m | |
4 | 6.11+3.60=9.70 | 1.70 | m | 10 | 3.52+3.16=6.68 | 1.12 | m | |
5 | 5.48+3.76=9.23 | 1.46 | m | 11 | 3.50+2.63=6.13 | 1.33 | m | |
6 | 5.15+3.96=9.11 | 1.30 | m | |||||
‘Castor’ | 1 | 8.59+4.84=13.42 | 1.77 | m | 7 | 5.13+3.13=8.26 | 1.64 | m |
2 | 7.49+3.33=10.81 | 2.25 | m | 8 | 4.29+3.52=7.81 | 1.22 | sm | |
3 | 5.97+4.71=10.68 | 1.27 | m | 9 | 4.12+3.58=7.70 | 1.15 | m | |
4 | 6.19+3.87=10.06 | 1.60 | sm | 10 | 4.52+2.86=7.38 | 1.58 | m | |
5 | 4.93+4.05=8.98 | 1.22 | sm | 11 | 3.45+2.79=6.24 | 1.24 | m | |
6 | 5.36+3.29=8.65 | 1.63 | m | |||||
‘SN Hongtaige’ | 1 | 7.98+5.18=13.16 | 1.54 | m | 7 | 5.22+2.91=8.13 | 1.79 | m |
2 | 7.51+4.24=11.75 | 1.77 | sm | 8 | 4.16+3.54=7.69 | 1.17 | m | |
3 | 5.70+4.75=10.45 | 1.20 | m | 9 | 4.43+2.81=7.24 | 1.57 | m | |
4 | 6.20+3.99=10.19 | 1.55 | m | 10 | 3.53+3.17=6.69 | 1.11 | m | |
5 | 6.41+3.15=9.55 | 2.03 | m | 11 | 3.91+2.54=6.45 | 1.54 | m | |
6 | 5.04+3.65=8.69 | 1.38 | m | |||||
‘Pink Passion’ | 1 | 7.83+5.81=13.64 | 1.35 | sm | 7 | 4.86+3.59=8.45 | 1.36 | m |
2 | 6.00+4.97=10.98 | 1.21 | m | 8 | 4.24+3.88=8.12 | 1.09 | m | |
3 | 6.21+3.94=10.14 | 1.58 | m | 9 | 4.49+2.97=7.46 | 1.51 | m | |
4 | 4.90+4.44=9.33 | 1.10 | m | 10 | 4.05+3.17=7.22 | 1.28 | m | |
5 | 5.64+3.57=9.21 | 1.58 | m | 11 | 3.58+3.23=6.81 | 1.11 | m | |
6 | 5.22+3.42=8.64 | 1.52 | m | |||||
‘Red River’ | 1 | 7.17+5.64=12.81 | 1.27 | m | 7 | 4.71+3.78=8.48 | 1.25 | m |
2 | 6.44+4.75=11.19 | 1.36 | m | 8 | 4.52+3.67=8.19 | 1.23 | m | |
3 | 7.03+3.92=10.95 | 1.79 | m | 9 | 4.78+2.71=7.49 | 1.77 | m | |
4 | 6.54+3.92=10.46 | 1.67 | sm | 10 | 3.49+3.16=6.65 | 1.10 | m | |
5 | 5.40+4.09=9.48 | 1.32 | m | 11 | 3.17+2.42=5.59 | 1.31 | m | |
6 | 4.97+3.73=8.70 | 1.33 | m |
Table 3
Karyotype formulas and parameters of 6 cultivars of F. hybrida
品种 Cultivars | 核型公式 Karyotype formula | 染色体相对长度组成 Chromosome relative length composition | 核不对称系数 Asymmetrical karyotype coefficient /% | 染色体长度比 Chromosome length ratio | 平均臂比 Arm ratio average | 臂比大于2的比例 Scale of arm ratio>2 /% | 核型分类 Classification |
---|---|---|---|---|---|---|---|
‘Gold River’ | 2n=4x=44=33m+11sm | 6L+18M1+15M2+5S | 59.07 | 2.62 | 1.47 | 6.82 | 2B |
‘White River’ | 2n=4x=44=1M+34m+9sm | 6L+15M1+14M2+9S | 59.30 | 2.21 | 1.46 | 2.27 | 2B |
‘Castor’ | 2n=4x=44=35m+9sm | 4L+23M1+14M2+3S | 60.05 | 2.60 | 1.52 | 6.82 | 2B |
‘SN Hongtaige’ | 2n=4x=44=32m+12sm | 8L+19M1+12M2+5S | 60.07 | 2.54 | 1.52 | 9.09 | 2B |
‘Pink Passion’ | 2n=4x=44=38m+6sm | 5L+23M1+13M2+3S | 57.00 | 2.71 | 1.35 | 2.27 | 2B |
‘Red River’ | 2n=4x=44=37m+7sm | 6L+16M1+16M2+6S | 58.22 | 2.84 | 1.42 | 4.55 | 2B |
Supplementary table 1 Chromosome and karyotype data of early taxa of the genus Freesia
物种名称 Species | 发现 Discovery | 染色体倍性 Chromosome ploidy | 核型参数 Karyotype parameters | 参考文献 Reference |
---|---|---|---|---|
F. refracta‘Fischer’ | Taylor(1926) | 二倍体Diploid | 首次报道染色体数目 Chromosome number first reported:2n=2x=22 | [ |
(1)F. x hybrida (2)F. refracta,F. alba(=F.muirii) F. caryophyllacea(=F.elimensis) F. cf. speciosa | (1)Lawrence(1945) (2)Goldblatt(1971) | 二倍体Diploid 三倍体Triploid 四倍体Tetraploid | 染色体数目 Chromosome number: 2n=2x=22, 2n=3x=33, 2n=4x=44. | [ |
F. refracta | 王丽等(1988) | — | 首次报道具体核型情况,发现该种有6对亚中着丝粒染色体、4对中着丝粒染色体和1对端着丝粒染色体。 The specific karyotype was reported for the first time.It was found that the species had 6 pairs of subcentric chromosomes,4 pairs of metacentric chromosomes and 1 pair of terminal centromeric chromosomes. | [ |
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