Abstract: Some detective methods of proteins through two-dimensional electrophoresis are discussed in this paper. We compare two silver stain methods (diamine and non-dianime silver stain) used in our lab before. And the diamine silver stain method improved by us has the following strengthens:(1) the background is clearer;(2) sensitivity is stronger largely, and the minimal quantity detected is up to 10fg, which can separate the proteins expressed very little and detect clearly the proteins that other methods cannot detect.

Key words: protein, two-dimensional electrophoresis, diamine silver stain