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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 604-613.doi: 10.7525/j.issn.1673-5102.2021.04.016

• Research report • Previous Articles    

Cloning and Functional Analysis of Rice Gene OsRPK2

Fu-Zhen ZHANG, Fang-Hui LIU, Xin-Xin CHEN, Jing SUN, Rong-Chao GE()   

  1. College of Life Science,Hebei Normal University,Shijiazhuang 050024
  • Received:2020-02-28 Online:2021-07-20 Published:2021-03-24
  • Contact: Rong-Chao GE E-mail:grcgp@sina.com
  • About author:ZHANG Fu-Zhen(1989—),female,graduate,mainly engaged in salt-related gene cloning and functional studies.
  • Supported by:
    National Natural Science Fund of China(30900104);Hebei Natural Science Fund(C2016205158)

Abstract:

Receptor-like protein kinase gene OsRPK1 plays an important role in the resistance signal transduction of rice. OsRPK2 gene, which is highly homologous to OsRPK1, was amplified and transformed into Arabidopsisthaliana by the overexpression vector p1300:35S:OsRPK2. The stress resistance analysis of 35S:OsRPK2Arabidopsis homozygous showed that the germination rate of OsRPK2 overexpressed Arabidopsis was significantly lower than that of the wild-type under salt, ABA and PEG stress. The root and plant growth of the OsRPK2 overexpressed Arabidopsis seedlings showed more obvious inhibition than that of wild-type. Physiological detection of 35S:OsRPK2Arabidopsis showed that the chlorophyll content decreased more significantly, proline increased less and malondialdehyde content increased more significantly than the wild-typeafter salt treatment. These physiological mechanisms of OsRPK2 overexpression Arabidopsis significantly reduce the stress resistance. Through qRT-PCR detection of the OsRPK2 overexpressed Arabidopsis, the overexpression of OsRPK2 inhibited the expression of SADSOS3 and FRY genes significantly. Therefore, OsRPK2 gene may affect Arabidopsis resistance through SOS and CDPK signaling pathway.

Key words: OsRPK2, receptor-like protein kinase, stress resistance, physiological detection, signaling pathway

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