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Bulletin of Botanical Research ›› 2001, Vol. 21 ›› Issue (1): 126-130.

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ANALYSIS OF GENETIC VARIATION WITHIN AND AMONG BETULA PLATYPHYLLA PROVENANCES AND PROVENANCE DIVISION USING RAPD MARKERS

JIANG Jing, YANG Chuan-ping, LIU Gui-feng, LIU Yu-xi, REN Xu-qin   

  1. Northeast Forestry University, Harbin 150040
  • Received:2000-09-01 Online:2001-03-15 Published:2016-06-14

Abstract: RAPD was used to detect genetic variation among 17 provenances, including 152 individuals. 233 loci were amplified using 14 random primers. The differentiation of the percentage of polymorphic loci among different provenances was evident, ranging from 20.17% to 32.19%, among which there were Maoershan provenance and Qingyuan provenance with the highest PPL and Chaoer provenance with the lowest PPL. Most of the variation (56.47%) occurred among individuals within provenances, and 43.53% was attributed to differences among provenances. The dendrogram of genetic relationship was constructed according to genetic distances among provenances. The result revealed that the provenances from Northeast and others from North and Northwest were respectively clustered into different groups. At the same time, provenance division was completed on the basis of geographic climatic factors and genetic distances.

Key words: RAPD, Genetic variation, B.platyphylla Suk, Provenance, Provenance division