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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (1): 69-77.doi: 10.7525/j.issn.1673-5102.2019.01.009

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Genetic Diversity of Ampelocalamus luodianensis in Different Microhabitats

LIU Ji-Ming, GUAN Rui-Ting, WANG Min, CHEN Jing-Zhong, TONG Bing-Li, WU Meng-Yao   

  1. College of Forestry, Guizhou University, Guiyang 550025
  • Received:2018-09-19 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    International Science and Technology Cooperation Project of Guizhou Province[(2013)7010];Science and Technology for Social Development Project of Qiannan State

Abstract: By random amplified polymorphic DNA(RAPD) markers analysis, 5 kinds of karst microhabitats of Ampelocalamus luodianensis was conducted to study genetic diversity and genetic structure. Through 8 RAPD primers, 87 discernible DNA fragments in all weighted from 200-3 000 bp were cloned, and 68 in those were polymorphic loci, with percentage of polymorphic loci at the species level, PPL=78.16%, Nei's index H=0.291 3, and Shannon's Information Index I=0.431 0. The A.luodianensis grew on soil surface(TM) possessed the highest level of genetic diversity(PPL=47.13%, H=0.195 3 and I=0.283 4), while the A.luodianensis in stone crevice exhibited the lowest levels of genetic diversity(PPL=39.08%, H=0.147 4 and I=0.219 2). Coefficient of genetic differentiation (Gst) was 0.415 1(from the POPGENE32), and the gene flows(Nm) was 0.704 5. Among the 5 microhabitats, genetic identity varied from 0.777 3-0.999 4, genetic distance was different from 0.000 6-0.251 9, and the mean of them were 0.865 6-0.148 3 severally. Genetic diversity in niches at mean value was with PPL of 43.91%. Coefficient of genetic variation in niches was with Gst of 0.4054. The results indicated that differences of microhabitats increased the genetic diversity of A.luodianensis.

Key words: Ampelocalamus luodianensis, RAPD-PCR, genetic diversity, microhabitat, endangered mechanism, conservation strategies

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