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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (2): 278-283.doi: 10.7525/j.issn.1673-5102.2018.02.016

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Gnentic Diversity and Phylogeographic Structure of Triosteum pinnatifidum based Chloroplast DNA Sequence rbcL-accD

LIU Hai-Rui1,2, GAO Qing-Bo1, ZHANG Fa-Qi1, XING Rui1, CHI Xiao-Feng1, CHEN Shi-Long1   

  1. 1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008;
    2. University of the Chinese Academy of Sciences, Beijing 100039
  • Received:2017-09-21 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    Project supported by "Applied Basic Research Programs of Qinghai Province"(2016-ZJ-761);National Natural Science Foundation of China(31400322)

Abstract: Triosteum pinnatifidum is perennial herb belonging to Triosteum (Caprifoliaceae) with the distribution in northwest Qinghai-Tibetan Plateau and adjacent mountain regions and Japan. It's endemic species of East Asia. We studied the sequence features, genetic variation and phylogeographic structure of T.pinnatifidum based on chloroplast DNA intergenic region rbcL-accD. Analysis of molecular variance for the whole populations revealed that 72.28% of total genetic variation occurred among populations, and 27.72% within populations. Dominant haplotype H2 had wide distribution while the private haplotypes could be found in different populations. There was no phylogeographic structure(NST<GST) and low gene flow(Nm=0.096), indicating the geographic isolation and variable habitat created by the special topography or ecological environment on the Qinghai-Tibetan Plateau and adjacent mountain regions may form current distribution of haplotypes. T.pinnatifidum experienced nonsignificant species selection or expansion. Indeed, we speculate there were different refugias for T.pinnatifidum during glaciations, it spread to current locations during interglaciation or postglaciation. To know the details of refuges and the migration routes, more work are needed in future survey.

Key words: Triosteum pinnatifidum, genetic variation, phylogeographic structure, cpDNA sequence

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