Abstract: Calli and the regenerative plants were induced from young leaves, immature seeds and hypocotyl of Swertia franchetiana H. Smith. Three kinds of media, MS, B 5 and N 6 were set up in the experiment. Calli were established with the highest frequency onto MS medeium containing 3mg/L 2,4-D and 0.5mg/L 6BA. The regenerative plants are obtained with the highest frequency onto MS medeium, Containing 0.5mg/L 6-BA and 0.2mg/L NAA. The induction and maintenence and differentiation of the calli were affected markly by the basic media and compound 2,4-D and 6-BA in the media etc. The Oleanolic acid content in calli of Swertia franchetiana H. Smith were determined by HPLC method.

Key words: Swertia franchetiana H. Smith, Tissue culture, Oleanolic acid