Abstract: Using Agrobacterium rhizogenes with Ri-plsmid (PRiA4b) to be medium,the binary vector PBTC 8 (T-DNA with CMV-cp and TMV-cp genes) was transforred in to cultivated tomato of Heilongiiang province. The method of hypocotyl,apical half of epicotyl and cutting cotyledon sections was used to induct hairy roots. Agropion and mannopine were detected in half of these hairy roots by electrophores. Some hairy roots were get by the method of sdlection medium with kanomycine resistance. The adventitious bud and regeneration plant were induced from cullus with kanomycine resistance on infected cotyledon piece. Detecting the alkaloid, PCR and DIBA (Dot Immunobinding Assy),it certified there were TMV-cp and CMV-cp genes, Agropion and mannopin in the regeneration plant We got deuble traslative plant.

Key words: Ri plasmid, Tomato, TMV-cp and CMV-cp genes.