Characteristics of Rhizosphere Soil Microecological Environment of Different Ecological Restoration Vegetation in Arsenic Sandstone Areas

doi:10.7525/j.issn.1673-5102.2023.04.006

Abstract

Abstract:

To provide an effective supplement for ecological restoration technology in the Arsenic sandstone area， the microecological soil environment in Pinus tabulaeformis and Caragana korshinskii plantation， naturally restored grassland， and abandoned land were studied respectively. The changes in microbial community structure and microbial biomass carbon， nitrogen and phosphorus contents， soil enzyme activities related to nutrient conversion， and soil chemical properties were analyzed in different vegetation types. The correlation between soil microbial community structure and soil microecological environment was discussed， then the main influencing factors of soil microhabitat types were explored in different ecological restoration vegetation. The results showed that：（1）The soil dissolved organic carbon（C） content of P. tabulaeformis plantation was the highest of the four vegetation types， which was 4.26 mg·kg^-1. The soil available nitrogen（N） content of the C. korshinskii plantation was the highest， which was 11.69 mg·kg^-1. However， there was no significant difference in soil available phosphorus（P） content among P. tabulaeformis plantation， C. korshinskii plantation and natural restored grassland. （2）The enzyme activities related to nutrient conversion and soil microbial biomass C， N and P of P. tabulaeformis plantation were significantly higher than those of other vegetation types. （3）The relative abundance of soil fungi was the highest in P. tabulaeformis plantation， which was 31.36%. The relative abundance of Gram-positive bacteria in the soil of the C. korshinskii plantation and abandoned land was the highest， which were 35.73% and 37.27%. The relative abundance of soil Gram-negative bacteria increased with the decrease of available organic C. It was the lowest in P. tabulaeformis plantation（36.82%） and the highest in the natural restored grassland（42.13%）. （4）The relative abundance of soil fungi was positively correlated with enzyme activities related to nutrient conversion. Gram-negative bacteria of preferred unstable C sources were less correlated with soil microecological environmental factors. Still， Gram-positive bacteria were significantly negatively correlated with the indexes of soil C conversion. （5）The Redundancy analysis showed that alkaline phosphatase was the most explainable environmental factor， and it was positively correlated with fungi and negatively correlated with Gram-positive bacteria（P<0.01）. In conclusion， the stability of the microecological environment of P. tabulaeformis plantation was relatively high in the ecological restoration vegetation types of the Arsenic sandstone area， which might be used as the optimal ecological restoration tree species in the Arsenic sandstone area. The synergistic effect of alkaline phosphatase and fungi in rhizosphere soil microecological environment might effectively regulate environmental restoration in Arsenic sandstone areas.

Key words: vegetation type, microbial community structure, microbial biomass, soil enzyme activity, soil available nutrient

CLC Number:

S791.254

Jikang XU, Yanhong HE, Tingyan LIU, Longfei HAO, Shengxi ZHANG, Zhaoyi LI. Characteristics of Rhizosphere Soil Microecological Environment of Different Ecological Restoration Vegetation in Arsenic Sandstone Areas[J]. Bulletin of Botanical Research, 2023, 43(4): 531-539.

Figures/Tables 7

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植被类型 Vegetation types	pH	溶解性有机碳 Dissolved organic carbon（DOC） /（mg·kg^-1）	有效氮 Available nitrogen（AN） /（mg·kg^-1）	有效磷 Available phosphorus（AP） /（mg·kg^-1）
油松 Pinus tabulaeformis	8.45±0.03b	4.26±0.15a	5.67±0.34c	3.32±0.23b
柠条 Caragana korshinskii	8.42±0.02b	3.58±0.17b	11.69±0.35a	2.84±0.08b
草地 Grassland	8.67±0.04a	3.99±0.15ab	7.42±0.44b	2.66±0.21b
撂荒地 Abandoned land	8.72±0.05a	3.87±0.08ab	5.60±0.33c	5.04±0.34a

植被类型 Vegetation types	β-1，4-葡萄糖苷酶 β-1，4-glucosidase（BG） /（μmol·h^-1·g^-1）	β-1，4-N-乙酰葡糖胺糖苷酶+亮氨酸氨基肽酶 β-1，4-N-acetylglucosidase+leucine aminopeptidase （NAG+LAP）/（μmol·h^-1·g^-1）	碱性磷酸酶 Alkaline phosphatase（ALP） /（μmol·h^-1·g^-1）
油松 Pinus tabulaeformis	15.61±1.38a	23.34±2.20a	35.17±3.46a
柠条 Caragana korshinskii	14.37±1.93ab	13.38±1.89b	18.47±1.52b
草地 Grassland	11.49±0.77b	13.40±2.11b	19.64±1.47b
撂荒地 Abandoned land	6.03±0.89c	8.92±0.84b	14.16±1.54b

植被类型 Vegetation types	微生物生物量碳 Microbial biomass carbon（MBC） /（mg·kg^-1）	微生物生物量氮 Microbial biomass nitrogen（MBN） /（mg·kg^-1）	微生物生物量磷 Microbial biomass phosphorus（MBP） /（mg·kg^-1）
油松 Pinus tabulaeformis	630.98±31.17a	20.13±1.71a	7.59±0.62b
柠条 Caragana korshinskii	408.97±25.38b	12.86±0.65b	4.41±0.40c
草地 Grassland	465.95±32.69b	10.68±0.74b	4.53±0.36c
撂荒地 Abandoned land	417.95±23.25b	11.04±1.01b	18.97±1.21a

植被类型 Vegetation types	真菌 Fungi	革兰氏阳性菌 Gram-positive bacteria	革兰氏阴性菌 Gram-negative bacteria
油松 Pinus tabulaeformis	31.36±0.43a	31.83±1.01b	36.82±0.86c
柠条 Caragana korshinskii	24.50±0.46b	35.73±1.39a	39.77±1.34ab
草地 Grassland	25.31±1.09b	32.56±1.13b	42.13±0.46a
撂荒地 Abandoned land	24.55±0.27b	37.27±0.49a	38.18±0.56bc

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