Regulation of Salicylic Acid on Tolerance to Saline Alkali Stress at Seedling Stages of Betula platyphylla

doi:10.7525/j.issn.1673-5102.2023.03.007

Abstract

Abstract:

To investigate the effects of salicylic acid（SA） on growth and secondary production synthesis of Betula platyphylla seedling under saline alkali stress， three treatments were applied to birch seedlings： water treatment（control）， 200 mmol·L^-1 NaHCO₃， 200 mmol·L^-1 NaHCO₃+360 μmol·L^-1 SA. The relative conductivity， chlorophyll fluorescence parameters， osmotic content， anti-stress enzyme activity， the relative expression of key genes of triterpene synthesis pathway in the middle leaves of birch seedlings in response to different treatments were detected respectively， and the total triterpenoids， flavonoids and polyphenols of birch seedlings were analyzed. The results showed that： ①under the 200 mmol·L^-1 NaHCO₃ treatment， then 360 μmol·L^-1 SA treatment significantly reduced the relative conductivity of the leaves of birch seedlings， improved the F_v/F_m value of chlorophyll fluorescence parameters of birch seedlings， increased the content of the osmotic regulator proline and soluble protein， and enhanced the activities of antioxidant enzymes（SOD， POD， CAT， APX） in birch seedlings to a certain extent. ②200 mmol·L^-1 NaHCO₃ saline alkali stress promoted the upregulation of the expression of key genes of triterpene synthesis pathway FPS， SS， SE， BPX and BPW in birch seedlings， and significantly promoted the synthesis of polyphenols in birch seedlings in the early stage， and significantly promoted the accumulation of total triterpenoids and flavonoids in the late stage of saline-alkali stress（P<0.05）. The application of SA under saline-alkali stress promoted the upregulation of HMGR， FPS， SE， and BPX gene expression in birch seedlings， and promoted the synthesis of flavonoids in the early stage of stress， and significantly promoted the accumulation of total triterpenes in the late stage of stress， which was 34% higher than that of the saline-alkali stress group and 47% higher than that of the water treatment on 7^th day. Overall， under saline-alkali stress， SA with suitable concentration improves the tolerance to saline-alkali of birch seedlings by adjusting osmotic equilibrium， alleviating light inhibition， enhancing the antioxidant defense system of birch seedlings， and regulating substance metabolism.

Key words: Betula platyphylla, salicylic acid, tolerance to saline-alkali, secondary production

CLC Number:

S792.153

Jingzhe WANG, Chaokui NIU, Xinyuan LIANG, Chenjing SHEN, Jing YIN. Regulation of Salicylic Acid on Tolerance to Saline Alkali Stress at Seedling Stages of Betula platyphylla[J]. Bulletin of Botanical Research, 2023, 43(3): 379-387.

Figures/Tables 5

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References 40

1	RAHMAN M M， MOSTOFA M G， KEYA S S，et al.Adaptive mechanisms of halophytes and their potential in improving salinity tolerance in plants［J］.International Journal of Molecular Sciences，2021，22（19）：10733.
2	HANIN M， EBEL C， NGOM M，et al.New insights on plant salt tolerance mechanisms and their potential use for breeding［J］.Frontiers in Plant Science，2016，7：1787.
3	李金霞，储博彦，尹新彦，等.白桦研究现状综述［J］.湖北农业科学，2019，58（2）：5-9，32.
	LI J X， CHU B Y， YIN X Y，et al.Research progress on Betula platyphylla ［J］.Hubei Agricultural Sciences，2019，58（2）：5-9，32.
4	丁福安.白桦人工用材林营造技术与措施［J］.牡丹江师范学院学报（自然科学版），2014，89（4）：38-39.
	DING F A.Technology and measures of Betula platyphylla plantation construction［J］.Journal of Mudanjiang Normal University（Natural Sciences Edition），2014，89（4）：38-39.
5	李影，徐林琳，张一彤，等.白桦酯酸及其衍生物的生物活性及作用机制研究进展［J］.中草药，2020，51（24）：6377-6390.
	LI Y， XU L L， ZHANG Y T，et al.Research progress on biological activities and mechanism of betulinic acid and their derivatives［J］.Chinese Traditional and Herbal Drugs，2020，51（24）：6377-6390.
6	PINZARU I， SARAU C， CORICOVAC D，et al.Silver nanocolloids loaded with betulinic acid with enhanced antitumor potential：physicochemical characterization and in vitro evaluation［J］.Nanomaterials，2021，11（1）：152.
7	HOENKE S， HEISE N V， KAHNT M，et al.Betulinic acid derived amides are highly cytotoxic，apoptotic and selective［J］.European Journal of Medicinal Chemistry，2020，207：112815.
8	李春晓，尹静，詹亚光，等.水分、氮肥及MeJA处理对白桦三萜积累特性的影响［J］.西北植物学报，2012，32（1）：155-161.
	LI C X， YIN J， ZHAN Y G，et al.Effects of water，nitrogen and methyl jasmonate treatment on triterpenes accumulation in birch（Betula platyphylla Suk.）［J］.Acta Botanica Boreali-Occidentalia Sinica，2012，32（1）：155-161.
9	李可鑫，赵微，徐林琳，等.温度胁迫对白桦丛生苗次生产物合成及抗逆酶积累的影响［J］.北京林业大学学报，2021，43（7）：31-39.
	LI K X， ZHAO W， XU L L，et al.Effects of temperature stress on the accumulation of secondary metabolites and defensive enzymes in multiple shoots of Betula platyphylla ［J］.Journal of Beijing Forestry University，2021，43（7）：31-39.
10	YIN J， LIANG T， WANG S Y，et al.Effect of drought and nitrogen on betulin and oleanolic acid accumulation and OSC gene expression in white birch saplings［J］.Plant Molecular Biology Reporter，2015，33（3）：705-715.
11	KHAN M S S， ISLAM F， CHEN H，et al.Transcriptional coactivators：driving force of plant immunity［J］.Frontiers in Plant Science，2022，13：823937.
12	夏方山，毛培胜，闫慧芳，等.水杨酸对植物种子及幼苗抗逆性的影响［J］.草业科学，2014，31（7）：1367-1373.
	XIA F S， MAO P S， YAN H F，et al.Effects of salicylic acid on stress resistance of seeds and seedling［J］.Pratacultural Science，2014，31（7）：1367-1373.
13	李润枝，靳晴，李召虎，等.水杨酸提高甘草种子萌发和幼苗生长对盐胁迫耐性的效应［J］.作物学报，2020，46（11）：1810-1816.
	LI R Z， JIN Q， LI Z H，et al.Salicylic acid improved salinity tolerance of Glycyrrhiza uralensis Fisch during seed germination and seedling growth stages［J］.Acta Agronomica Sinica，2020，46（11）：1810-1816.
14	王艳，尹静，马泓思，等.钙离子在介导SA诱导白桦悬浮细胞三萜合成途径中的作用［J］.北京林业大学学报，2014，36（2）：51-58.
	WANG Y， YIN J， MA H S，et al.Role of calcium ion in mediating the triterpenoid synthesis induced by SA in suspension cells of Betula platyphylla ［J］.Journal of Beijing Forestry University，2014，36（2）：51-58.
15	包颖，魏琳燕，陈超.水杨酸和茉莉酸甲酯对盐胁迫下月季品种月月粉生理特性的影响［J］.云南农业大学学报（自然科学版），2020，35（6）：1040-1045.
	BAO Y， WEI L Y， CHEN C.Effects of exogenous salicylic acid and methyl jasmonate on the physiological characteristics of Rosa chinensis ‘Old Blush’ under salt stress［J］.Journal of Yunnan Agricultural University（Natural Science），2020，35（6）：1040-1045.
16	DEHNAVI A R， ZAHEDI M， LUDWICZAK A，et al.Foliar application of salicylic acid improves salt tolerance of sorghum（Sorghum bicolor（L.） Moench）［J］.Plants，2022，11（3）：368.
17	王立红，张巨松，李星星，等.外源水杨酸对盐胁迫下棉花幼苗光合作用的影响［J］.核农学报，2016，30（9）：1864-1871.
	WANG L H， ZHANG J S， LI X X，et al.Effects of exogenous salicylic acid on photosynthesis of cotton seedlings under salt stress［J］.Journal of Nuclear Agricultural Sciences，2016，30（9）：1864-1871.
18	高明远，甘红豪，李清河，等.外源水杨酸对盐胁迫下白榆生理特性的影响［J］.林业科学研究，2018，31（6）：138-143.
	GAO M Y， GAN H H， LI Q H，et al.The effect of exogenous salicylic acid on the physiological characteristics of Ulmus pumila plantlet under NaCl stress［J］.Forest Research，2018，31（6）：138-143.
19	沙汉景，刘化龙，王敬国，等.水杨酸调控作物耐盐性生理机制［J］.东北农业大学学报，2017，48（3）：80-88.
	SHA H J， LIU H L， WANG J G，et al.Physiological mechanism of salicylic acid regulating salt tolerance of crops［J］.Journal of Northeast Agricultural University，2017，48（3）：80-88.
20	YIN J， SUN L， LI Y，et al.Functional identification of BpMYB21 and BpMYB61 transcription factors responding to MeJA and SA in birch triterpenoid synthesis［J］.BMC Plant Biology，2020，20（1）：374.
21	YIN J， LI C X， ZHAN Y G，et al.The response of physiological characteristics，expression of OSC genes，and accumulation of triterpenoids in Betula platyphylla Sukto MeJA and SA treatment［J］.Plant Molecular Biology Reporter，2016，34（2）：427-439.
22	YIN J， LI X， ZHAN Y G，et al.Cloning and expression of BpMYC4 and BpbHLH9 genes and the role of BpbHLH9 in triterpenoid synthesis in birch［J］.BMC Plant Biology，2017，17（1）：214.
23	美合日古丽·米吉提，王玉成.东北白桦（Betula platyphylla Suk.）LEA基因的克隆及盐胁迫响应基因的鉴定［J］.分子植物育种，2017，15（7）：2570-2578.
	MIJITI M， WANG Y C.Cloning and identification of the LEA genes from Betula platyphylla Suk.in response to salt stress［J］.Molecular Plant Breeding，2017，15（7）：2570-2578.
24	叶查龙，颜斌，申婷婷，等.转BpmiR156基因白桦株系的耐盐性分析［J］.南京林业大学学报（自然科学版），2020，44（6）：147-151.
	YE Z L， YAN B， SHEN T T，et al.Analysis of salt tolerance in BpmiR156 overexpression Betula platyphylla ［J］.Journal of Nanjing Forestry University（Natural Sciences Edition），2020，44（6）：147-151.
25	张群，及晓宇，贺子航，等.白桦BpGRAS1基因的克隆及耐盐功能分析［J］.南京林业大学学报（自然科学版），2021，45（5）：38-46．
	ZHANG Q， JI X Y， HE Z H，et al.Cloning and salt tolerance analysis of BpGRAS1 gene in Betula platyphylla ［J］.Journal of Nanjing Forestry University（Natural Sciences Edition），2021，45（5）：38-46.
26	田增智，贺子航，王智博，等.白桦BpPAT1基因的表达模式及耐盐性分析［J］.北京林业大学学报，2021，43（10）：18-27.
	TIAN Z Z， HE Z H， WANG Z B，et al.Expression patterns and salt tolerance analysis of BpPAT1 gene in Betula platyphylla ［J］.Journal of Beijing Forestry University，2021，43（10）：18-27.
27	任春林，尹静，潘亚婕，等.水杨酸对白桦悬浮细胞中齐墩果酸积累及防御酶活性的影响［J］.中草药，2012，43（5）：972-977.
	REN C L， YIN J， PAN Y J，et al.Effects of salicylic acid on accumulation of oleanolic acid and defense enzyme activity in suspension cells of Betula platyphylla ［J］.Chinese Traditional and Herbal Drugs，2012，43（5）：972-977.
28	郝再彬，苍晶，徐仲.植物生理实验［M］.哈尔滨：哈尔滨工业大学出版社，2004：101-104.
	HAO Z B， CANG J， XU Z.Plant physiological experiment［M］.Harbin：Harbin Institute of Technology Press，2004：101-104.
29	萧浪涛，王三根.植物生理学实验技术［M］.北京：中国农业出版社，2005：152-223.
	XIAO L T， WANG S G.Experimental techniques in plant physiology［M］.Beijing：China Agriculture Press，2005：152-223.
30	李琳，焦新之.应用蛋白染色剂考马斯蓝D-250测定蛋白质的方法［J］.植物生理学通讯，1980（6）：54-57.
	LI L， JIAO X Z.Determination of protein using coomassie blue D-250 protein stain［J］.Plant Physiology Journal，1980（6）：54-57.
31	李合生.植物生理生化实验原理和技术［M］.北京：高等教育出版社，2000：164-165.
	LI H S.Principles and techniques of plant physiological and biochemical experiments［M］.Beijing：Higher Education Press，2000：164-165.
32	陈建勋，王晓峰.植物生理学实验指导［M］.广州：华南理工大学出版社，2006：70-73.
	CHEN J X， WANG X F.Experimental guidance in plant physiology［M］.Guangzhou：South China University of Technology Press，2006：70-73.
33	YIN J， REN C L， ZHAN Y G，et al.Distribution and expression characteristics of triterpenoids and OSC genes in white birch（Betula platyphylla Suk.）［J］.Molecular Biology Reports，2012，39（3）：2321-2328.
34	马泓思，潘亚婕，王艳，等.Ca²⁺在介导MeJA诱导白桦悬浮培养三萜合成中的作用［J］.植物研究，2015，35（1）：117-126.
	MA H S， PAN Y J， WANG Y，et al.Effect of Ca²⁺ on mediating the MeJA-induced synthesis of triterpenoid in suspension cells of Betula platyphylla Suk.［J］.Bulletin of Botanical Research，2015，35（1）：117-126.
35	王佺珍，刘倩，高娅妮，等.植物对盐碱胁迫的响应机制研究进展［J］.生态学报，2017，37（16）：5565-5577.
	WANG Q Z， LIU Q， GAO Y N，et al.Review on the mechanisms of the response to salinity-alkalinity stress in plants［J］.Acta Ecologica Sinica，2017，37（16）：5565-5577.
36	魏婧，徐畅，李可欣，等.超氧化物歧化酶的研究进展与植物抗逆性［J］.植物生理学报，2020，56（12）：2571-2584.
	WEI J， XU C， LI K X，et al.Progress on superoxide dismutase and plant stress resistance［J］.Plant Physiology Journal，2020，56（12）：2571-2584.
37	徐国前，张振文，郭安鹊，等.植物多酚抗逆生态作用研究进展［J］.西北植物学报，2011，31（2）：423-430.
	XU G Q， ZHANG Z W， GUO A Q，et al.Progress on the stress-resistant ecological function of plant polyphenols［J］.Acta Botanica Boreali-Occidentalia Sinica，2011，31（2）：423-430.
38	李波，方志坚，邬婷婷，等.盐碱胁迫下紫花苜蓿叶片蛋白组的初步分析［J］.草地学报，2019，27（3）：574-580.
	LI B， FANG Z J， WU T T，et al.Preliminary analysis of alfalfa leaf proteome under salt-alkali stress［J］.Acta Agrestia Sinica，2019，27（3）：574-580.
39	卫乐，姚爱兵，孙福华，等.盐碱胁迫对“丰花2号”花瓣多酚类物质的影响［J］.北方园艺，2021（17）：81-89.
	WEI L， YAO A B， SUN F H，et al.Effects of saline-alkali stress on polyphenols，flavonoids and anthocyanins of ‘Fenghua No.2’ rose petals［J］.Northern Horticulture，2021（17）：81-89.
40	李欣，李影，曲子越，等.bHLH转录因子在茉莉酸信号诱导植物次生产物合成中的作用及分子机制［J］.植物生理学报，2017，53（1）：1-8.
	LI X， LI Y， QU Z Y，et al.The molecular mechanism and the function of bHLH regulating jasmonic acid-mediated secondary metabolites synthesis［J］.Plant Physiology Journal，2017，53（1）：1-8.

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