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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (3): 429-435.doi: 10.7525/j.issn.1673-5102.2021.03.013

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Genetic Diversity Assessment of Rhododendron sinofalconeri with Genotyping by Sequencing(GBS)

Xu ZHANG1,2, Xiu-Jiao ZHANG1, Yong-Peng MA3, Zheng-Hong LI1, You-Ming WAN1, Hong MA1()   

  1. 1.Research Institute of Resources Insects,Chinese Academy of Forestry,Kunming 650233
    2.College of Forestry,Nanjing Forestry University,Nanjing 210037
    3.Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201
  • Received:2020-03-17 Online:2021-05-20 Published:2021-03-24
  • Contact: Hong MA E-mail:hortscience@163.com
  • About author:ZHANG Xu(1994—),male,past graduate,major in conservation biology of Rhododendron plants.
  • Supported by:
    Fundamental Research Funds for the Central Non-profit Research Institution of the Chinese Academy of Forestry(CAFYBB 2019ZB007);Biodiversity survey and assessment project of Ministry of Ecology and Environment(2019HJ2096001006);Ten Thousand Talent Program of Yunnan Province(2019);Technology Innovation Talent Project of Yunnan Province(2016HB007)


Rhododendron sinofalconeri has high ornamental value with its pure yellow flower and bell-shaped corollas clustered on the top of the branches. In recent years, due to the anthropogenic impact of road construction, illegal excavation and other human activities, its habitat was severely fragmented and its population was shrunk. Therefore, it was urgent to research on conservation biology of R.sinofalconeri. Single-nucleotide-polymorphism(SNP) site mining and genetic diversity analysis were performed on two surviving populations of Laojunshan(LJS) and Daheishan(DHS) using genotyping-by-sequencing(GBS) technology. As a consequence, analysis of the 103 133 high-quality SNPs revealed that the two populations had lower genetic diversity at the species level(Ne=1.308 6, Ho=0.187 8, and He=0.185 6), and higher genetic differentiation(Fst=0.176 5) at population level. The value of gene flow(Nm) between populations was 1.167 4. By cluster analysis, principal component analysis and population genetic structure analysis, 36 individuals were clustered into two different genetic groups. This study revealed the current situation of relatively low genetic diversity of R.sinofalconeri for the first time, which was helpful to further understand the endangered and scientific formulation of conservation measures.

Key words: Rhododendron sinofalconeri, GBS, SNP, Genetic structure, Reintroduction

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