Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (3): 436-440.doi: 10.7525/j.issn.1673-5102.2021.03.014
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Min CHEN1, Meng-Fei TIAN1, Chang-Yin FENG1, Xian-Ming MENG1, Meng LUO2, Fan-Song MU1()
Received:
2020-03-17
Online:
2021-05-20
Published:
2021-03-24
Contact:
Fan-Song MU
E-mail:fansong8867@nefu.edu.cn
About author:
CHEN Min(1992—),male,master,mainly engaged in plant active ingredient research.
Supported by:
CLC Number:
Min CHEN, Meng-Fei TIAN, Chang-Yin FENG, Xian-Ming MENG, Meng LUO, Fan-Song MU. Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC[J]. Bulletin of Botanical Research, 2021, 41(3): 436-440.
Table 3
Repeatability and stability test results of each component
测定成分 Determination of compounds | 重复性Repeatability | 稳定性Stability | ||
---|---|---|---|---|
保留时间 Retention time | RSD (%) | 平均峰面积 Average peak area | RSD (%) | |
草苁蓉纳拉苷 Baschnaloside | 17.193 | 0.501 | 9 161.39 | 0.29 |
草苁蓉苷B Rossicaside B | 40.077 | 0.531 | 3 340.73 | 0.75 |
Table 4
Result of recovery of baschnaloside(n=9)
测定成分 Determination of compounds | 样品量 Sample size(μg) | 加入量 Sample size(μg) | 测得量 Sample size(μg) | 回收率 Recovery(%) | 平均回收率 Recovery(%) | RSD(%) |
---|---|---|---|---|---|---|
草苁蓉纳拉苷 Baschnaloside | 101.01 | 81.54 | 177.55 | 97.26 | 97.86 | 1.01 |
100.39 | 81.54 | 180.93 | 99.45 | |||
101.04 | 81.54 | 176.58 | 96.71 | |||
100.88 | 102.39 | 200.27 | 98.52 | |||
100.54 | 102.39 | 196.93 | 97.04 | |||
101.10 | 102.39 | 197.49 | 97.05 | |||
100.82 | 127.07 | 221.89 | 97.37 | |||
100.46 | 127.07 | 223.53 | 98.24 | |||
100.05 | 127.07 | 225.07 | 99.10 |
Table 5
Result of recovery of rossicaside B(n=9)
测定成分 Determination of compounds | 样品量 Sample size(μg) | 加入量 Sample size(μg) | 测得量 Sample size(μg) | 回收率 Recovery(%) | 平均回收率 Recovery(%) | RSD(%) |
---|---|---|---|---|---|---|
草苁蓉苷B Rossicaside B | 201.04 | 161.27 | 353.31 | 97.52 | 96.55 | 1.23 |
200.20 | 161.27 | 348.47 | 96.40 | |||
201.21 | 161.27 | 349.48 | 96.41 | |||
200.19 | 202.80 | 397.99 | 98.76 | |||
200.59 | 202.80 | 382.99 | 94.94 | |||
201.18 | 202.80 | 391.99 | 97.03 | |||
200.77 | 290.13 | 470.90 | 95.93 | |||
200.06 | 290.13 | 475.19 | 96.94 | |||
200.15 | 290.13 | 466.28 | 95.10 |
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