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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (2): 252-257.doi: 10.7525/j.issn.1673-5102.2016.02.014

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Sequence and Expression Analysis of MYB Family Genes in Betula platyphylla Suk.

LIU Hui-Zi;SUN Dan;YU Ying;ZHANG Nan;WANG Chao*   

  1. The State-Owned Key Laboratory of The Northeast Forestry University,Harbin 150040
  • Online:2016-03-15 Published:2016-05-20

Abstract: The MYB transcription factor family is one of the important plant transcription factor family members, and they have the key function on the growth, development, cell wall biosynthesis and stress response in plant. We obtained the total of 17MYB genes, and by phylogenetic analysis, 17 MYB genes of birch belong to different subfamilies and groups. Among these genes, 10 MYBs belong to the 1R/4R subfamilies, and 7 MYBs belong to the 2R subfamily. BplMYB13 is homologous to the AtMYB46 which functions in the secondary cell wall biosynthesis. BplMYB15 and BplMYB26 belong to the group which involved in the stress response. BplMYB23 is homologous to the AtMYBs involved in the glucosinolate biosynthesis. BplMYB9, 21 and 22 have homologous with the AtMYBs functioned in phenylpropanoid biosynthesis. The expression patterns analysis among the development stages of the growth season and the tension wood and opposite wood bended for 6 h, and the normal wood were performed by real time RT-PCR. 17 MYB genes had the peak express levels from the middle of May to the middle of July, and the BplMYB13 maintained the high express levels among all the growth season in the cambium and xylem. The BplMYB13 may play the role in the cambium and xylem development. After 6 h bending, 14 MYBs were up-regulated in the tension wood compare with the opposite wood or normal wood, and 7 MYBs were increased expressed in the opposite wood than those in the normal wood. Therefore, these genes response to the artificial bending and play key roles in the physiology change during the xylem development response to the bending stress.

Key words: Betula platyphylla Suk., MYB, sequence analysis, expression analysis