Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (1): 75-84.doi: 10.7525/j.issn.1673-5102.2014.01.011

Previous Articles     Next Articles

Cloning and Gene Expression of 3-Hydroxy-3-Methylglutaryl-CoA Synthase Gene(AsHMGS) from Aquilaria sinensis(Lour.) Gilg

LIU Juan;XU Yan-Hong;YANG Yong;LIANG Liang;GAO Zhi-Hui;YANG Yun;ZHANG Zheng;SUI Chun;WEI Jian-He;*   

  1. 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193;2. Hainan Branch Institute of Medicinal Plant (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Medical Sciences & Peking Union Medical College,Wanning 571533;3.Shangdong University of Traditional Chinese Medicine,Jinan 250355
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-01-20 Published:2014-01-20
  • Contact: WEI Jian-He
  • Supported by:

Abstract: Homologous HMGS gene cDNA was isolated from the stem of Aquilaria sinensis(Lour.) Gilg through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technique, and named as AsHMGS. The nucleotide sequence of AsHMGS gene was 1 831 bp and contained an open reading frame (ORF) of 1 398 bp encoding 465 amino acids with a molecular weight of 51.4 kD, and the theoretical isoelectric point was 6.25. Representative motifs of AsHMGS and active site were deduced in the amino acids sequence of AsHMGS. The results of phylogenetic analysis suggested that the protein sequence of AsHMGS had high similarity to that of Arabidopsis thaliana, Arabidopsis lyrata subsp. lyrata, and Brassica juncea, followed by Camellia sinensis, Camptotheca acuminate, and Panax ginseng. The results of real-time PCR showed that the transcription of AsHMGS could be induced by methyl jasmonate.

Key words: Aquilaria sinensis, HMGS gene, sequence analysis, gene expression analysis

CLC Number: