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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (1): 61-68.doi: 10.7525/j.issn.1673-5102.2012.01.011

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Cloning and Expression Analysis of HMGR Gene from Taraxacum kok-saghyz

WANG Qi-Chao;LIU Shi-Zhong;XIAO Xian-Zhou;*   

  1. 1.College of Horticulture and Landscape Architecture,Hainan University,Haikou 571737; 2.Rubber Research Institute,CATAS,Haikou 571737
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20
  • Contact: XIAO Xian-Zhou
  • Supported by:

Abstract: Based on the design of degenerated oligonucleotides according to the conservative regions of nine 3-hydroxy-3-methylglutaryl-CoA reductases from nine plants and the total RNA extracted from Taraxacum kok-saghyz, a HMGR named TKHMGR was first obtained using the techniques of degenerate RT-PCR and RACE. Comparison of the amino acid sequence homology and system evolution analysis show that, TKHMGR belongs to the new members of the HMGR gene families. At the same time, the gene expression in different organizations was analyzed using fluorescence quantitative method. The cloning, bioinformatics analysis and expression analysis of this gene laid a foundation for further research on its function.

Key words: Taraxacum kok-saghyz(TKS), 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase(HMGR), gene cloning, sequence analysis, fluorescence quantitation

CLC Number: