Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (1): 47-53.doi: 10.7525/j.issn.1673-5102.2012.01.009

Previous Articles     Next Articles

Cloning and Analysis of the cat Associated Genes of Hevea brasiliensis and Banana in Hainan

YU Nai-Tong;SUN Yu-Juan;ZHANG Yu-Liang;LUO Zhi-Wen;LIU Zhi-Xin*   

  1. 1.College of Environment and Plant Protection,Hainan University,Haikou 570228; 2.Ministry of Agriculture,Key Laboratory of Tropical Crop Biotechnology,ITBB,CATAS,Haikou 571101
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20
  • Contact: LIU Zhi-Xin
  • Supported by:

Abstract: According to the existence of plant cat genes in GenBank, we designed a pair of degenerate primers, and amplified cat genes of banana, Hevea brasiliensis, yellow lantern pepper, pineapple, sugar cane, papaya from Hainan island. Ultimately, we cloned the rubber cat-1 and banana cat-2 genes, while the others not. Sequence analysis showed that open reading frame (ORF) of rubber cat-1 and banana cat-2 genes were both 1 479 bp, and encoded 492 amino acids. GenBank accession number were HQ660587 and HQ660588. Using bioinformatics software, the tertiary structures of rubber’s CAT-1 and banana’s CAT-2 proteins were predicated, which were similar to Exiguobacteriu Oxidotolerans (PDB code:2j2mA0) and Pseudomonas Syringae (PDB code:1m7sA), respectively. Phylogenetic analysis indicated that Hainan H.brasiliensis CAT-1 possessed the highest homology with Ricinus communis CAT-1 (XP_002521709.1), while banana CAT-2 was the highest homology with Elaeis guineensis CAT-2 (ACF06566.1).

Key words: Banana, Hevea brasiliensis, cat gene, cloning, sequence analysis

CLC Number: