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植物研究 ›› 2010, Vol. 30 ›› Issue (3): 369-372.doi: 10.7525/j.issn.1673-5102.2010.03.021

• 论文 • 上一篇    下一篇

7-木糖-10-去乙酰基紫杉醇的优化试验研究

张宇;姜守刚;祖元刚*;汪琢;付玉杰   

  1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 祖元刚
  • 基金资助:
     

Optimization of Extration of 7-xylosyl-10-deacetylpaclitaxel from Taxus cuspidata

ZHANG Yu;JIANG Shou-Gang;ZU Yuan-Gang*;WANG Zhuo;FU Yu-Jie   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: ZU Yuan-Gang
  • Supported by:
     

摘要: 在寻找东北红豆杉(Taxus cuspidata)中的抗癌的活性物质过程中,一种亲水性紫杉醇衍生物7-木糖-10-去乙酰基紫杉醇被筛选出来。目的是优化并建立红豆杉中7-木糖-10-去乙酰基紫杉醇的提取工艺及鉴定,本文以使用RP-HPLC测定的7-木糖-10-去乙酰基紫杉醇含量为指标,采用正交试验法对提取过程中乙醇浓度(A)、乙醇用量(B)、提取次数(C)、提取时间(D)4因素进行优化。结果从红豆杉中提取7-木糖-10-去乙酰基紫杉醇的最主要影响因素是乙醇浓度及固液比,高浓度、高用量有利于提取;提取时间和次数也极为重要,单因素实验结果以2 h/次和提取2次为最佳。结论N9(A3B3C2D1)、N8(A3B2C1D3)为优化选出的7-木糖-10-去乙酰基紫杉醇最佳提取工艺条件。

关键词: 7木糖10去乙酰基紫杉醇, HPLC, 提取分离, 鉴定, 正交实验

Abstract: In an effort to search for natural products from Taxus cuspidata with improved pharmacological features compared to paclitaxel,7-xylosyl-10-deacetylpaclitaxel has been isolated and identified by our group. The objective of the present study was to optimize and establish the extraction process of 7-xylosyl-10-deacetylpaclitaxel from T.cuspidata. RP-HPLC mothod was applied to determine the content of 7-xylosyl-10-deacetylpaclitaxel. The orthogonal test was adopted to examine the effects of the 4 factors including ethanol concentration, ethanol volume, number of extraction cycles and extraction time. Ethanol concentration and ethanol volume were essential factors influencing the extraction of 7-xylosyl-10-deacetylpaclitaxel. High concentration and amount of ethanol facilitated the extraction. Number or extraction cycles and extraction time were also important. Single-factor test indicated, 2 h and 2 cycles were the best. The optimum extractive condition are N9(A3B3C2D1) and N8(A3B2C1D3).

Key words: 7-xylosyl-10-deacetylpaclitaxel, HPLC, separation and purification, identification, orthogonal test

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