欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2020, Vol. 40 ›› Issue (6): 876-885.doi: 10.7525/j.issn.1673-5102.2020.06.010

• 研究报告 • 上一篇    

中国主栽樱花的分子系统发育分析

付涛1, 王志龙1(), 林乐静1, 林立1, 李文1, 袁冬明2   

  1. 1.宁波城市职业技术学院/浙江园林绿化技术协同创新中心,宁波 315100
    2.宁波市鄞州区林业技术管理服务站,宁波 315100
  • 收稿日期:2019-11-19 出版日期:2020-11-20 发布日期:2020-11-04
  • 通讯作者: 王志龙 E-mail:wangzhl01@163.com
  • 作者简介:付涛(1988—),男,硕士,实验师,主要从事植物育种研究。
  • 基金资助:
    宁波城市职业技术学院校内科研青年专项课题(ZZX18128);宁波城市职业技术学院校内科研青年专项课题(ZZX18122);宁波市重大科技攻关专项(2014C11002);宁波市自然科学基金项目(ZZT18104);宁波市自然科学基金项目(ZZT18104)

Molecular Phylogenetic Analysis of Cerasus Plants in China

Tao FU1, Zhi-Long WANG1(), Le-Jing LIN1, Li LIN1, Wen LI1, Dong-Ming YUAN2   

  1. 1.Zhejiang Landscape Technology Collaborative Innovation Center,Ningbo City College of Vocational Technology,Ningbo 315100
    2.Yinzhou Forestry Extension Center of Ningbo City,Ningbo 315100
  • Received:2019-11-19 Online:2020-11-20 Published:2020-11-04
  • Contact: Zhi-Long WANG E-mail:wangzhl01@163.com
  • About author:FU Tao(1988—),male,M.A,experimentalist,major in plant breeding research.
  • Supported by:
    School Scientific Research Youth Special Topics of Ningbo City College of Vocational Technology(ZZX18128);School Scientific Research Youth Special Topics of Ningbo City College of Vocational Technology(ZZX18122);Ningbo major science and technology project(2014C11002);Project of Natural Science Foundation of Ningbo(ZZT18104)

摘要:

以88份樱花为试验材料,利用核基因ITS和叶绿体间隔序列trnH-psbA进行测序,经过软件MEGA 6.0处理后,构建了系统发育树。结果表明,山樱群樱花品种系统发育比较混乱,这与其遗传背景比较复杂以及原始亲本系统发育关系较近有关,江户彼岸群和寒绯樱群的大多数品种能够聚类在一起,与山樱群品种能分得开,但涉及到很多杂交品种,ITS和trnH-psbA难以区分,表明DNA条形码技术难以应用到杂交品种的系统发育关系,此外,还可依据某些樱花品种特异的ITS或trnH-psbA序列长度对部分品种进行分子鉴定,也对部分种系不明的品种进行了修订。

关键词: 樱属, ITS, 叶绿体序列, 系统发育, 物种鉴定

Abstract:

To provide some molecular basis for the species classification and lineage location of cherry blossoms mainly planted in China, with 88 cherry blossoms, by nuclear gene ITS and chloroplast intergenic sequence, trnH-psbA were sequenced, and after processed by software MEGA 6, phylogenetic tree was constructed. The phylogenetic development was confused in Cerasus serrulata group, which is related to the complex genetic background and the close phylogenetic relationship among the primitive parental, meanwhile, it could be clustered together in most of the cultivars C.subhirtella group or C.campanulata group, and could be separated from the cultivars of the Cerasus serrulata group, however, it was difficult to distinguish in many hybrids using ITS and trnH-psbA, which indicated that DNA barcoding technology was difficult to apply to the phylogenetic relationship of hybrids, in addition, some cherry cultivars could be identified by ITS or trnH-psbA sequence length, and some unidentified varieties were also revised.

Key words: Cerasus, ITS, chloroplast sequence, phylogenetic, species identification

中图分类号: