植物研究 ›› 2021, Vol. 41 ›› Issue (4): 614-625.doi: 10.7525/j.issn.1673-5102.2021.04.017
收稿日期:
2020-08-29
出版日期:
2021-07-20
发布日期:
2021-03-24
通讯作者:
李为民
E-mail:763730163@qq.com
作者简介:
赵雪艳(1986—),女,讲师,博士研究生,主要从事植物的解剖结构以及资源开发利用方面的研究。
基金资助:
Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI()
Received:
2020-08-29
Online:
2021-07-20
Published:
2021-03-24
Contact:
Wei-Min LI
E-mail:763730163@qq.com
About author:
ZHAO Xue-Yan(1986—),female,lecturer,doctor,Research interests: anatomical structure of plants and resource exploitation and utilization.
Supported by:
摘要:
百合属植物是多年生的宿根草本植物,具有重要的观赏价值,有的种可食用也可药用。本研究对百合属4种植物(细叶百合、川百合、卷丹和百合)花的转录组进行测序,并结合前期的野百合的转录组数据进行比较转录组分析,鉴别花发育相关基因。并筛选出5种植物的直系同源基因,进而检测正选择基因,分析它们对环境的适应性。通过组装分别在细叶百合、川百合、卷丹以及百合花中获得了44 565、51 413、41 638和 44 716个unigene。为了了解unigene功能信息,将其在公共数据库中进行比对和注释,发现了13个花发育相关基因。另外,在5个物种间共获得8 247对直系同源基因,并计算其Ka、Ks及Ka/Ks值。在5个物种中有33对直系同源基因受到了强烈的正选择作用,随后对其进行GO、KEGG功能富集分析及基因功能注释,发现这些基因主要是与抗性以及生物合成相关的基因。
中图分类号:
赵雪艳, 李思锋, 柏国清, 李为民. 百合属5种植物的比较转录组分析[J]. 植物研究, 2021, 41(4): 614-625.
Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI. Comparative Transcriptome Analysis of Five Species of Lilium[J]. Bulletin of Botanical Research, 2021, 41(4): 614-625.
表2
百合属5种植物转录组注释信息
野百合 L. brownii | 细叶百合 L. pumilum | 川百合 L. davidii | 卷丹 L. lancifolium | 百合 L. brownii var. viridulum | |
---|---|---|---|---|---|
Nr | 28 104 | 28 681 | 31 641 | 26 438 | 29 932 |
Swiss-prot | 17 739 | 18 283 | 20 390 | 17 381 | 19 951 |
GO | 6 494 | 6 785 | 7 398 | 6 475 | 7 360 |
KOG | 14 682 | 14 958 | 16 619 | 14 371 | 16 268 |
KEGG | 11 984 | 12 557 | 13 570 | 11 960 | 13 247 |
所有注释的基因数目 All annotation unigenes | 28 187 | 28 709 | 31 827 | 26 527 | 30 025 |
注释基因的百分比 The percentage of annotated unigenes(%) | 68.89 | 55.23 | 61.54 | 56.96 | 48.93 |
图1
百合属5种植物转录组GO注释1.代谢过程;2.细胞过程;3.单-生物过程;4.生物调节;5.定位;6.生物过程调节;7.刺激反应;8.细胞成分组织或生物起源;9.发育过程;10.多细胞生物过程;11.信号;12.复制;13.生殖过程;14.多—生物过程;15.生物过程的负调节;16.生物过程的正调节;17.生长;18.免疫系统过程;19.生物粘附;20.节律过程;21.解毒作用;22.细胞杀伤;23.移动;24.细胞;25.细胞部分;26.细胞器;27.膜;28.膜部分;29.细胞器部分;30.大分子复合物;31.细胞连接;32.细胞外区域;33.膜—封闭腔;34.病毒体;35.病毒粒子部分;36.胞外基质;37.类核;38.细胞外基质组分;39.细胞外区域部分;40.超分子纤维;41.催化活性;42.结合;43.转运活性;44.结构分子活性;45.核酸结合转录因子活性;46.分子传感活性;47.信号传感活性;48.分子功能调节体;49.抗氧化活性;50.转录因子活性,蛋白结合;51.电子载体活性
表3
鉴别的花发育相关的基因
基因ID Gene ID | 基因 Gene | 蛋白质 Protein |
---|---|---|
Unigene0017918 | MADS2 | MADS盒转录因子2 MADS-box transcription factor 2 |
Unigene0027916 | MADS6 | MADS盒转录因子6 MADS-box transcription factor 6 |
Unigene0004890 | MADS14 | MADS盒转录因子14 MADS-box transcription factor 14 |
Unigene0039518 | MADS16 | MADS盒转录因子16 MADS-box transcription factor 16 |
Unigene0040377 | AGL8 | 无性MADS盒蛋白AGL8 Agamous-like MADS-box protein AGL8 |
Unigene0003323 | AGL15 | 无性MADS盒蛋白AGL15 Agamous-like MADS-box protein AGL15 |
Unigene0026977 | AGL104 | 无性MADS盒蛋白AGL104 Agamous-like MADS-box protein AGL104 |
Unigene0031138 | AGL19 | 无性MADS盒蛋白AGL19 Agamous-like MADS-box protein AGL19 |
Unigene0025453 | AGL30 | 无性MADS盒蛋白AGL30 Agamous-like MADS-box protein AGL30 |
Unigene0031144 | SOC1 | MADS盒蛋白SOC1 MADS-box protein SOC1 |
Unigene0042321 | EJ2 | MADS盒蛋白EJ2 MADS-box protein EJ2 |
Unigene0008053 | LIM15 | 减数分裂重组蛋白DMC1同源体 Meiotic recombination protein DMC1 homolog |
Unigene0027070 | GlsA | 无性生殖细胞形成蛋白GlsA Gonidia forming protein GlsA |
表4
强烈正向选择的基因的KEGG通路
KEGG通路 KEGG pathway | 通路编号 Pathway ID | 基因注释 The annotation of gene |
---|---|---|
糖基磷脂酰肌醇(GPI)-锚的生物合成 Glycosylphosphatidylinositol(GPI)-anchor biosynthesis | K05283 | GPI-锚定壁转移蛋白亚型4[可可] GPI-anchored wall transfer protein isoform 4[Theobroma cacao] |
萜类骨架生物合成 Terpenoid backbone biosynthesis | K06013 | CAAX异戊二烯基蛋白酶1同源物[非洲油棕] CAAX prenyl protease 1 homolog[Elaeis guineensis] |
mRNA监督途径 mRNA surveillance pathway | K12881 | RNA与输出因子结合蛋白2[涧生花烛] RNA and export factor-binding protein 2[Anthurium amnicola] |
苯丙素的生物合成 Phenylpropanoid biosynthesis | K00430 | 过氧化物酶57[巨桉] peroxidase 57[Eucalyptus grandis] |
植物-病原菌相互作用 Plant-pathogen interaction | K13457 | 疾病抗性蛋白RPM1类[枣椰树] disease resistance protein RPM1-like[Phoenix dactylifera] |
RNA运输 RNA transport | K12881 | RNA与输出因子结合蛋白2[涧生花烛] RNA and export factor-binding protein 2[Anthurium amnicola] |
剪接体 Spliceosome | K12881 | RNA与输出因子结合蛋白2[涧生花烛] RNA and export factor-binding protein 2[Anthurium amnicola] |
代谢途径 Metabolic pathways | K05283+K00430 | GPI-锚定壁转移蛋白亚型4[可可];过氧化物酶57[巨桉] GPI-anchored wall transfer protein isoform 4[Theobroma cacao];peroxidase 57[Eucalyptus grandis] |
次生代谢物的生物合成 Biosynthesis of secondary metabolites | K00430 | 过氧化物酶57[巨桉] peroxidase 57[Eucalyptus grandis] |
附表 百合花发育相关的基因的序列信息
基因ID Gene ID | 序列信息 Sequence information |
---|---|
Unigene0017918 | GCCGCGGCAAGATCGAGATCAAGCGGATCGAGAACTCCACCAACCGCCAGGTCACCTTCTCCAAGCGCCGCAACGGCATCATCAAGAAAGCCCGTGAGATCAGCGTCCTCTGTGAGGCCCAGGTCTCCGTCGTCATCTTCTCCAGCTCCGGCAAGATGTCCGAGTACTGCAGCCCCTCCACCTCTCTACCGAAGATCCTTGAGAGGTATCAGGTGAACTGCGGCAAGAAGATCTGGGATCCAAAGCATGAGCACCTGAGCGCGGAGATTGATAGGATCAAGAAGGAGAATGATAATATGCAGATCCAGCTGAGGCATCTGAAGGGAGAGGATCTGAACTCGTTGCAGCCGAAGGAGCTGATCCCAATCGAGGAGGCGCTGGAGAATGGCATCAGGGGCGTCCGGGAGAAGCAGAATGATTTTCTGAGGATGCTCAAGAAGAATGAAAGGATACTGGAAGAGGATAACAAACGGTTAACTTATATACTGCACCATCAGCAACTGGCAATGGATGAAAATATGAGGAACTTGGAATTTGCATATCATAATAAAGATGGGGATTTCGGTTCCCAGATGCCAATGGCCTTTCGTGTGCAGCCAATCCAGCCTAATTTACATGAGGACAAGTAGTATGAGCATCTCCTGTGCCTGCTATTTTATTTAGACATCGAGTATCTAAAGAAATTTATTATCTGTTATTCTACATGCTTAATGTTATTATTTGATATTCTTATCTCTTGATGTTCATGTAGTAATTTGATAGTATAATCGTAATATTGGACTTCATAGTAAAGAAGCTAG |
Unigene0027916 | GGGTAAAAAAGAGTAAAGTTGAGATCAAATCCAACAAATCTTGCAACCCTTGTCTCTCCTCTCCTTATATTCCCTTCCTTTCTATAATTATCATCCTTTCTCCATTTCTTGAGAGAGAAGAGAGAGAGAGGGGATGGGGAGGGGAAGAGTGGAGCTGAAGAGGATTGAGAACAAGATCAACCGCCAAGTCACCTTCTCGAAACGCCGGAACGGGCTGCTGAAAAAGGCCTATGAGCTCTCGGTCCTCTGCGATGCGGAGGTCGCTCTCATCATCTTCTCCAGCAGAGGCAAGCTTTATGAGTTCGGCAGCGCAGGGACCAGTAAAACTCTTGAGCGTTACCAACGTAGTTGTTACACTTCTCAAGATACTAATGCCGTTGATCGCGAAACCCAGAGTTGGTATCAAGAAATGTCCAAATTGAAGATGAAATTTGAGTCTCTACAACGCTCCCAAAGGCATTTATTGGGAGAGGACCTCGGACCATTGAACGTGAAAGAATTACAGCAATTGGAACGCCAACTTGAATCTGCTTTGTCACAAGCTAGGCAAAGAAAGACTCAATTGATGTTGGAACAAGTGGACGAACTGCGTAAAAAGGAACGCCATCTCGGTGAAATTAACAAGCAACTAAAAAACAAGCTTGAGGCTGAGGGGTTTGACTTCCGATCTTTGCAAGTATCATGGGATACGGAGGATGTTGTTGGAGGAAATACATTTTCGCAGCATCATATGCCATCTAGTTCGATGACTATTGAACCCACTCTTCAGATGGGGTATCACCAATTTGTTCCTGCTGAAGAAGCTTCAATGCAAAGGAACACCGGTGCGGAGAATAATTTCATGCTAGGTTGGGTTCTTTGATTGAAGATTGGTTAGTACGTGTTATGTCTCGTAAAAGATCATCGGATTGTTTAACTATGTTTTCAGTTGCTTTCTTAGCTATTGTATTTCGACTTCTTTCAAATTTGCTGCTGTGGCTGATCTGCTTGTATCCGAAAGTTTTTGTTGTTGAAAATTTCATGCAGATTAATTACTGGATGGTTAGATGTATTGAATTCGGATGAATAAATTGTGTAAGGTGATGCTGTTTTTATGATGCAGCATT |
Unigene0004890 | AAAAGTTATGCATACCAGTACATAAACCTATACATAGATATAGTTATGCCCCCTTTCTTCGAGAGGGGTAACACCATAGCAAAACCAGATCTCTGCTGCTATAAGAATGATAGTGAAACTTAGCAGAATTCCCCGTAAAATAATTATGATGGGCGGGGAAGGTGATAGCTTATCTGTTAACGTGGCGAAGCATCCATGGCGGTAGTGAGCTGCTGCCAACACGCATCAGCGGCTGTTCAGCTGCCCCCTCGTCTGCGTCGCTGCTGCTGCTGCCTCTTTCTGGGTAGATTCCAGCGTTTTGGACGGGGTGCAAATGGGGCATTAGGAAAGGAGGGGGCCTTGTCATGGGCTGTTGTCCTTGTAGCTCCCAGTGATCTTGCTGTGTCAAAGCCTCCATCCTCTGGAACTCCATGAGCTTTTTCTCCATTATAGTGTTTTGTTCCCTCAGTGATTTCTCTGTTCGCTGGAGCTCGGTAATCGAATCAAACAATAGTTGGTGCTTCCTTGATCTTACATGCTTGAAAGATAATTCGAGCTGCTGCTCTAGTTGTTGGAGTTGTTTCAGAGTCAAGTCCTCAAGCTGCTCCCCCATGAGATGTCTTTGGCTTTTCTGCAAGGTTTCAACCTTAGCCTTCAGTCTCCCGTACTCTTGACACCAGTTTCCCTGTGCTTCAGGATATGTGTCTGTCACTGCAGTTTCTGCTTGAGAATAAAGCTCGTAACGTTCGAGAATCCTTTCCATG |
Unigene0039518 | AAGAAGATCGAAAACTCAACGAATCGGCAGGTCACTTACTCGAAGCGCCGGACTGGAATCATCAAGAAGGCGACTGAGCTCACTGTGCTCTGTGATGCCGAGGTCTCTCTCCTTATGTTCTCCAGCACCGGGAAGCTGTCAGAGTTCTGCAGCCCCTCCACAGACACGAAAAAGATCTTCGATCGCTACCAGCAGCTGTCTGGTATCAACCTCTGGAGCGCGCAATACGAGAAAATGCAAAACACTTTGAACCATCTGAGCGAGATCAACCGCAACCTTCGCAAGGAGATCAGCCAGAGGATGGGGGAGGAGCTGGATGGATTGGACATCAAGGACCTGCGCGGTCTTGAGCAAAATTTGGACGAAGCGCTCAAGCTCGTTCGTCACCGCAAGTATCATGTGATCAACACTCAGACAGAGACTTACAAGAAAAAGGTCAAAAACTCGGAAGAAGCACACAAGAACTTGCTCCGTGACCTGGTGAATCGAGAGATGAAAGATGAGAATCCAGTCTATGGTTATGTGGACGAAGACCCCAGCAACTATGATGGCGGCCTTGGTCTGGCCAATGGGGCTTCTCATCTGTACGAGTTCCGAGTCCAACCGAGCCAGCCAAACCTGCACGGGATGGGTTATGGCTCCCATGATCTCCGACTGGCTTGAAACCCTAATTCTGTAGGGTGCAGTGA |
Unigene0040377 | GAATAACGCTCATAGCGTTCCAGAATTCTTTCCATGCTAGCGTCAGTAGAGTACTCGTACAGCTTGCCCTTGGCGGAGAATACAACAAGAGCGACCTCTGCATCGCAGAGGACAGAGATCTCATGCGCCTTCTTCAGCAGCCCCGACCGCCGCTTCGAGAAGGTGACTTGCCGGTTGATCTTGTTCTCTATCCGCTTCAGCTGCACTCGCCCCCGGCCCATCTCCAGAAAACCCTAAAACTATTGAGATGGATGAGATATCCTCAGCTTATTGAAGAGTGAGGGGGAGACAGAA |
Unigene0003323 | CGCCAGAGGGAGTGAGTGAGACCCATAGAGGGAAATTTTGGAGGGAAAAAAATGGGGAGGAGGAAGAGGGAGATAAGGCGGATTGAGGACACCAACAGCCGCCAGGTCACTTTCTTGAAACGCGGCAGTCGGCTGCTGAAGAAGGCTTCAGAGCTCTCCGTGTTTTGCGATGCCGAGGTTGCGCTGATCGTCTTCTCACCCATGGGCAATCTCTGCGAGTTCTCCAGCTGCAGGTTAGGGTGGTATCGGTTCCAATCAAGGAGTATCCTGCTTGGCCGCCTGCAACGCGGACCAGCTGATTGGAATTTTGTTTATAATGCTGCTACTTTTAGAATAAC |
Unigene0026977 | AGAGCTTTCTTCTCAATTACCAAAAGTATAATATTTCCTCTTGTGGTTCTAAATACAAATATTGCTCATAGATGGAGCCTATACCTCAACTCTACTTTTAGTGTATATTTAGCATATACAAATATATTTTCTAATTCTTTTATCATTATGGTCCTTACCCTATGTTCGCATTTATCTGAACAATGCTGCCTTCATAGGCAGCAGCGGTTCCTTCATACGCAGCAGCGGAGCATGATGCATTCTCGTTCCCTGGCATATGCGAGCAGTTCGCAGATGGATCCAGCTGCTCATGTGGCACCATCGATGGCAGCTCCGCTGATGCCATTGAATGCTGGATCATCGGGTAGGGACCAGTGGGGATGAGAGACGAGAGGAGCTCAGTAGAGGTGTATGCCTGGTGGTGGTGCCACATGTCGTTTTGACTGTTTATGTGACAACCATCCATGCTGGGATCCACCTGTAGCCCTGTGTTCTGGTGGATCGGGTTGTAAATCCCTTGATCCCTGAGGGACATTAGTAGATCGGACCCAACAAACATCTGATGGCCTGAGTTGGATGTTCCCTCAGTGATCCATTGGACCATCTCGTTTCCGAACGCATTGGGGATTCCTTCTTGATGAGGTTGCATGTACATCTGCATATTAGATGTAGAGGGATCGAATGTCGACAGATGATTATTTAGCAAATATTTCTTTCTCTCGTTAACTCTTGTTAGTGTCTCAATGAGGAACTTTTCACAAGTTTGCAGCTCATCCATTGATCCCATACTCAAAGGATCCGGCTCGAAACACCTTAATTGATTTTCCGAGTGTTGTAATTGTTCTTGGTATCTACTAATCTCTTGGTGGAGCTCCTCCATGTTGGAGTTACACACCCCAGGATTGGCTATTTGGGCCGCCATGTCACTCTCGCACTTTAGCTTCTTCAAGGTTCTGATTAAGTATTCTCTATTTTGGATCACTCCTCCCCGATCGTGCTCCGGGAGGCTGATGTATCGAGCGAGAACATCCTCGATCCTTCGACGGCCGGAGAAGTGGCTGAGGCGGCCGGAGGGGGAGAACATGATTAGGGCAATGTCGATGTCGCAGAGGATGGAGAGCTCGTAGGCCTTCTTGATGAGGCCATTGCGACGCTTGGAGAAGGTGACTTGGCGATTGGTGTTGTTCTCGATCTTCTTGATTTGGAGCTTTACGCGGCCCA |
Unigene0031138 | AACATGAGATAGATATCAACTTCAACAGGCCTTCAGAAGGAAATATGCAGCAACTGCAGTGTGAAGCCGAGGGCCTCGCGAAGAGGATTCAAATCCTTGAAGATTATAAACGGAAGGTGATGGGTGAAAATCTGGAAACGTGTTCAATTGAAGAACTTCATGAATTAGAGGGTCAATTAGAGCAAAGTTTGAGCAAAATCAGACAAAAGAAGAATCACGAACAATCAGAGCAAATTGTGCAGTTAAAAGAACAGTTGTCTTCTTGCATTTTGCTCACAAAAATTCAGGAGAGGATTCTGTTGGAGAAGGAGAGGATTCTGTTGGAGAAGAATGCATCATTACAAGAAAAGGACACTGATTCTACAGAAGTTCTAGATGCTGACATGGAGGTACTCACGGAGCTGTTTATCGGGAGGCCTGGGTGCAGTAAAA |
Unigene0025453 | TTAGTTTACATACATATCGTAACACTCAAAAATGAATCCGTTACACAAATAATCGAATCTCTATTTCTCAGATATAATGTACAACCTGAGGACAATATGCTTTATCTATCATGACACTATACAACGAAAGTTTTATATACGACGTATAACAGCTGAAATAATTTGAGAATCAGTATTGTTAAAACAGGTCCTGTATTTGTATAAGAGTAGCTACATACATCCCCACTATTAGTCCAGTGCCCGTCGTTGAATTAAATGACGATACCGGCGTATTTTCATTGACTGGAGGCGAGCCTAGGGGATGAACACTGCACAGGCTCAGGTGCTGTATGATCTACTGAATCTGGTCTGGTTGTTGGGGGTAAGGATGGTTATCAAACATGGGAACAGACGTGGAAGCCCATGTCTCGATGTTGGCATCATATTCAGGCCCAGGGGGCTCAAAACCATTAACTTGGTAATCAACCCGGCCCTCCTGTAAGCTAATTTCTGCATCGGGGTTGAACTTATTCTCGCTAAGCAAATTCATTCCATACGACTGATTAGGGTAGTGGCCTCCAAGCTGCAGTTGCAAGCATGAGTTTTGGCTCAAATCTAACTGCTCTTTGGTGTCTACCTGCTTTCCCATGGTAAAGTAACCAGGAAAGCCTTGAAGAGACCCTTCTGCGGAGCACTCAATATCTCTTTGGGAAAGCAGATTAGGGCCTTCTGGAAGCATCATGTGTGAACCATTACAGTCCTGAAGCCATTGCATATGTGACAGTTGTTGCTCGCCGTTAATTGTCAGGGGTAAATGCATACCATTCTGGAACTGGCCAGAACATTCTAATGACATAATTTGCTGCTTTCCCAGGTTTTCCTTGTGCATTCTGATTCGGTTCACAGACTCTTCAAGGGATTCCTCCATTGCTCTGATATGATCTATACTGTTGATCTTATCGATATCTTTCCAATAACTTAGTCTCTTTTCAGCGTCTGATATCTGAGCTTGCAAGAACCTTGAGTGATTTGTCAATTCCTCAATTGTTTGGGTGCTTGAACCTAGAAAATCTTGTATATTGACATCATGGTCGAGCTTCTTGAATGTCTTCTTCAGTGCTTCAAGGCTCTCCAACTTCCTCTTTGCCCTCTCCTGTGGAGTTAACTGAGCAAACTTCGAAATGACCTCCTCAATATTGCTGCGATCTCCCAGGCACATTGTTGGCTTCCCAGTCGGCGAGAACATGAGAAGGATGATATCAATGTCGCACAAAATCGATAGCTCCTTGGCCTTCTTTAGGATCCCGGCTCTCCGTTTAGAGTAAGTCACCTGCCGCGCACTGGTATTCTCCAATCTCTTGATTTTCAACTTCACCCTCCCCATCTGATCACCCTTGTGCTTAAAAGGGAATCGAGATAACCCTCGAAAAACAAAGGACCCAAATGCAATCAAGGCAACTGTCTCAAAATTCGTCCGATGTGCAACCGAATCAATAGATGGTATGAAAAAATTGCGAGAACCCAGAATTCGGAAGGGAAGAAGGACGCTGATGAAGATCAAAATAGGCAATTAGAATGAAAATTGAGGGTATCCCAATTCAGAGAATTGGACAACAAGCACGGGTTGGGAGTTCCGGAGATAGAACATCAAGAACACGATCTGT |
Unigene0031144 | GCAATAGAGCAACCCTTGAGCGAGGGAGGGAGTGACAATCATAGAGGGAATTTTTTTGGAGGGAAAAAATGGCGAGGAGGAAGAGGGAGATAAGGCTGATTGAGGACACCAACAGCCGTCAGGTGACTTTCTCGAAGCGCGGCAGTGGGCTGCTGAAGAAGGCTTTCGAGCTCTCCGTGCTTTGCGATGTCGAGGTTGCGTTGATCGTCTTCTCACCCAAGGGCAAGCTCTGCGAGTTCTCCAGCTCCAGCATGCAAAGTACAATCGATCATTATCTGATGCATGAGAAAGATATCGACATAAGCAGGCTTTCAGAAAAAAATATGCAGCAAATGGAATGTGAATCTGCGGATCTCATGAAGAGCATTGAAATCCTTGAAGATTATAATAGGATGCGGTTGTGTGAAAATCTGGAAACATGTTCAATTGAAGAACTTCATGAAATAGAGGGTCAGTTAGAGCAAAGTTTGAGAACAATCAGACAAAAGAAGAATCACATTATATCAGAGCAAATTGCGCAGTTAAAAGAAAAGGAGAGGATTCTGTTGGAGAAGAATGCATCATTACAAGAAAAGTTCAAGGAAAAAACTCCACAACAGAAAACTGATTCTACGGAAGTTCTAGATGCTGACATGGAGGTGCTCACTGAGCTGTTTATTGGGAGGCCTGGGTGCAGCAAAACCCGAAGCATGCCAAGAGGTTGATTGAAATGCATGAGGCGCCGGGCAAGAGCCGGGAGCGGCAGATCGGACAATTCACATCGACCCCGCTTTGTCTTGACGGGGGAGGCGAACCGGACGACGGTCAGCTTTGTCTTGACGGGGGCGGCGACAGAGCGAGCGGAGGGGAAGCGGAGCACGGCGGTGATGATGGGGTTCGTGGAGGAGA |
Unigene0042321 | TTTGAGATCACGCAACTGATCAAGTATTATCTGAGTCTTTTTCGACCTGATCTGATTTAAAGACTTCTCTAGTTGATTGTCAAGTTGTTCGAGTTCCTTGTTACTAAGTTGACCCAAGTTCTCGCCAAGGAGATTTCTACAATTCCACGGTTAAGAGTTTCATTAGCGAAATGGTTTACAACATAGATATTGCTACTACGGATTACAGATTTG |
Unigene0008053 | GTGGTGGAATTGAGACACTACAGATCACAGAAGCCTTCGGTGAATTCAGGTCTGGAAAGACACAGATAGCTCACACCCTCTGCGTGTCAACACAGTTGCCGGTGAGCATGCATGGAGGCAACGGCAAGGTCGCTTACATCGACACAGAAGGCACATTCCGACCAGACCGCATTGTACCAATTGCAGAGAGATTTGGAATGGACGCGAGTGCAGTACTGGACAATATCATATATGCGCGGGCATACACCTACGAGCACCAGTACAACCTTCTTCTTGCTCTAGCTGCCAAGATGTCTGAAGAGCCTTTCCGGCT |
Unigene0027070 | TCAGCCTCTCATTTCGGGGGAAAGAGGAAGCCCTAGAGCGCCGCCGCGTACCGATCCTCTCAATCGCTGCTCACAGATAAATTGAAGAGCTCTTCACCAGCTACCACCTGCTCACTTTATTTAAATTGTCAAGCCCATCGTCTGGGAGTACAATGAATGCCAAGAGTTGTCTCCTACTTACCTATTCATCTGAGGTTTTGAATGGAGTACCCTTATTCTTCGCATCTAATTGCCTCCCTGTGAAGGCTGTGAATCAAGAACCTGCTGGGCATGCGTTCCATGATGCTGCGCTTAAACTTTGCGGTTTGTTCGAAGAAGATGTAGTCACAGATGATCAAAGCGAGTCATCAGATGATCGAGGTCCAGCATTTATGGCATCATCAGATTCATATAGAAGCAAAAGCAAAAAGAAATCTGCTGGTAAAAGTGACCAACAAGACCACTATGCATTGCTAGGCTTGGGCCATCTACGTTTTCTGGCCACTGAGGAGCAGATACGTAAAAGTTACCGGGAAACTGCTTTGAAGCATCATCCTGACAAACAGGCTGCCCTTCTCCTTACTGAAAAAACAGAAGCTTCAAAGCAAGCAAAGAAGGATGAGATTGAAAACCATTTCAAAGATATTCAAGAAGCTTATGAAGTTCTAATTGATCCCGTGAGAAGAAGGGTCTATGACTCAACAGATGAGTTTGATGATGAAGTGCCGAGTGACTGTGCACCACAAGACTTCTTTAAGGTGTTTGGCCCAGCATTTATGAGGAACGGGAAATGGTCTGTAGTCCAGCCAGTTCCTTCCTTGGGGGATGATAAAACTTCACTGGAAGAGGTGGACAACTTTTACGACTTTTGGTATGCATTTAAGAGTTGGAGGGAGTTCCCACATGCAGATGAGTTCGAGCTAGAGCAATCAGAGTCTCGTGATCATAAGAGATGGATGGAAAGGCAGAATGCAAAGCTGAGAGAAAAGGCGAGGAAGGAAGAGTATGCACGTGTTCGTTCCCTTATTGATAATGCTTACAAGAGGGACCCCAGACTCCTTCGGAGAAAGGAACAGGAGAAAGCAGAGAAGCAGAGGAGAAAAGAGGCAAAGTATATGGCGAAGAAATTGCAAGAAGAAGAGGCAGCTAGAGCTGTTGAGGAAGAGCGGCTCCGCAAGGAGGAGGATGAAAAAAAAGCAGCAGAGGCTGCTGTGATTAATAAGAAGATAAAGGAAAAGGAGAAGAAGCTCTTGCGCAAGGAGAAGACTCGCCTTCGCACGTTATCAGTATCACTAGTGTCAGATTCTTTGCTTGATCTAACGGAGGATGATGTGGAAAAAACATGCAACTCTTTTGGATTGGAGCAGCTTCGTCATCTCTGTGACGGCATGGAAGGTAGGGAAGGAATTGAGAGGGCTCAACTGCTCAAAGCTGCAGTCAGTGGTGATATGTTGGAGATCTCTAAGAAGGAACCTAATGATTTGAAACCAAATGGTTCCACGAATTCGGGCCCGAAAAGTAATGGTTCTGTAACTTCAGCGAAACCAGTAATTATGATGTCCAGCTACGAAAAGAAAGAGAAGCCTTGGGGTAAGGAAGAGATAGAGTTGCTGAGAAAGGGTATCCAGAAATATCAGAAGGGGACATCTCGTAGGTGGGAAGTTATTTCAGAGTATATCGGCACTGGGAGATCGGTTGAAGAAATCCTAAAAGCCACAAAGACTGTTCTTCTCCAAAAGCCTGATTCTTCTAAAGCTTTTGACTCCTTCCTTGAGAAAAGGAAGCCGGCGAAAGCCATTGTGTCACCCCTTACGACCAGACTTGAATCAGAAGGTTCCACTGTTGAGGCAGGAGATGCATCTTCTAAATCAATACCAACACCGTCTTTGAGCAGCAGCAGTCCTGAAAAGCCAGACGGTACTCCTGTTTCCCTTCCCAATGGGGTTCCTTCTGTCCCTGAACAAGACACATGGTCTGCTACCCAAGAAAGAGCTCTCATTCAGGCGCTAAAGACTTTCCCAAAGGATGTGAATCAGCGTTGGGAACGGGTTGCTGCTGCTATTCCCGGGAAAACCATGAACCAGTGCAGAAAGAAATTTTTATCAATGAAGGAGGACTTTCGAAGCAAGAAGAGTGGTTAAGTCTTTTTTCTTTTGCAGGTAAGTTCAATATAGAACTAAAAAAAGATGACCTCTTCATCATGGTGCTGAAATTTTCCTCATTACTGGGCTTAGCTGCAGTTATATCTGGGTGCTCTTTTTCCCGCAACAGAAGTTTTTGTGATGCTTTAGGATGTAGAACTAGATGGGTATGTTTATTTAAGACACAACTTTGGTGAGATCTGACAGTTGCTACTCTTTCATATGTATGAAAGTTTTGCAAGCTCCTTGATGGGAGTTGGAAGCCCAGGCTCCTGACAAACAAATGGTTGCTGAAACATCAGACTGGAGTATAATCATCAAACCTAAAGGTATATTTATTTCTTAATGATCTCGAGAAATTTATTCATGTGATGCTGTTCAAAAGAGTTGGTGTAGCTCTATGTCGGCAATTTTGCAGTGACAATTATTTAGCCATCTGATGC |
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