百合属5种植物的比较转录组分析

doi:10.7525/j.issn.1673-5102.2021.04.017

植物研究 ›› 2021, Vol. 41 ›› Issue (4): 614-625.doi: 10.7525/j.issn.1673-5102.2021.04.017

百合属5种植物的比较转录组分析

赵雪艳, 李思锋, 柏国清, 李为民()

陕西省西安植物园，陕西省植物研究所，陕西省植物资源保护与利用工程技术研究中心，西安 710061

收稿日期:2020-08-29 出版日期:2021-07-20 发布日期:2021-03-24
通讯作者: 李为民 E-mail:763730163@qq.com
作者简介:赵雪艳（1986—），女，讲师，博士研究生，主要从事植物的解剖结构以及资源开发利用方面的研究。
基金资助:
陕西省重点研发计划项目—农业领域(2018NY-88);陕西省科学院科技计划项目(2020K-28)

Comparative Transcriptome Analysis of Five Species of Lilium

Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI()

Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources，Xi’an Botanical Garden of Shaanxi Province，Institute of Botany of Shaanxi Province，Xi’an 710061

Received:2020-08-29 Online:2021-07-20 Published:2021-03-24
Contact: Wei-Min LI E-mail:763730163@qq.com
About author:ZHAO Xue-Yan(1986—),female,lecturer,doctor,Research interests: anatomical structure of plants and resource exploitation and utilization.
Supported by:
Key technology R&D program of Shaanxi province(2018NY-88);Science and technology program of Shaanxi academy of sciences(2020K-28)

摘要/Abstract

摘要：

百合属植物是多年生的宿根草本植物，具有重要的观赏价值，有的种可食用也可药用。本研究对百合属4种植物（细叶百合、川百合、卷丹和百合）花的转录组进行测序，并结合前期的野百合的转录组数据进行比较转录组分析，鉴别花发育相关基因。并筛选出5种植物的直系同源基因，进而检测正选择基因，分析它们对环境的适应性。通过组装分别在细叶百合、川百合、卷丹以及百合花中获得了44 565、51 413、41 638和 44 716个unigene。为了了解unigene功能信息，将其在公共数据库中进行比对和注释，发现了13个花发育相关基因。另外，在5个物种间共获得8 247对直系同源基因，并计算其Ka、Ks及Ka/Ks值。在5个物种中有33对直系同源基因受到了强烈的正选择作用，随后对其进行GO、KEGG功能富集分析及基因功能注释，发现这些基因主要是与抗性以及生物合成相关的基因。

关键词: 百合属, 转录组, 花发育基因, 直系同源基因, 正选择基因

Abstract:

Lilium are perennial herbaceous plants with important ornamental value and some species are edible and medicinal. In the present study， the transcriptome of four species of Lilium（L. pumilum， L. davidii， L. lancifolium and L. brownii var. viridulum） was sequenced respectively， and the genes which involved in flower development were analyzed. Combined with the previous transcriptome data of L. brownii， the orthologous genes were selected by comparative transcriptome analysis. Positive selections were screened and their environmental adaptability were analyzed respectively. 44 565， 51 413， 41 638 and 44 716 unigenes were obtained from L. pumilum， L. davidii， L. lancifolium and L. brownii var. viridulum respectively by assembly. In order to understand the function of unigene， the unigenes were compared and annotated in the public database. 13 genes related to the development of flower were identified. A total of 8 247 orthologous genes were obtained in five species， and Ka， Ks and Ka/Ks values were calculated. 33 pairs of homologous genes were strongly positive selected in five species. Subsequently， GO and KEGG enrichment analysis and gene function annotation were carried out， and it was found that these genes were mainly related to plant defense and biosynthesis.

Key words: Lilium, transcriptome, flower development gene, orthologous gene, positive gene

中图分类号:

S644.1

赵雪艳, 李思锋, 柏国清, 李为民. 百合属5种植物的比较转录组分析[J]. 植物研究, 2021, 41(4): 614-625.

Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI. Comparative Transcriptome Analysis of Five Species of Lilium[J]. Bulletin of Botanical Research, 2021, 41(4): 614-625.

图/表 9

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表4

附表　百合花发育相关的基因的序列信息

基因ID Gene ID	序列信息 Sequence information
Unigene0017918	GCCGCGGCAAGATCGAGATCAAGCGGATCGAGAACTCCACCAACCGCCAGGTCACCTTCTCCAAGCGCCGCAACGGCATCATCAAGAAAGCCCGTGAGATCAGCGTCCTCTGTGAGGCCCAGGTCTCCGTCGTCATCTTCTCCAGCTCCGGCAAGATGTCCGAGTACTGCAGCCCCTCCACCTCTCTACCGAAGATCCTTGAGAGGTATCAGGTGAACTGCGGCAAGAAGATCTGGGATCCAAAGCATGAGCACCTGAGCGCGGAGATTGATAGGATCAAGAAGGAGAATGATAATATGCAGATCCAGCTGAGGCATCTGAAGGGAGAGGATCTGAACTCGTTGCAGCCGAAGGAGCTGATCCCAATCGAGGAGGCGCTGGAGAATGGCATCAGGGGCGTCCGGGAGAAGCAGAATGATTTTCTGAGGATGCTCAAGAAGAATGAAAGGATACTGGAAGAGGATAACAAACGGTTAACTTATATACTGCACCATCAGCAACTGGCAATGGATGAAAATATGAGGAACTTGGAATTTGCATATCATAATAAAGATGGGGATTTCGGTTCCCAGATGCCAATGGCCTTTCGTGTGCAGCCAATCCAGCCTAATTTACATGAGGACAAGTAGTATGAGCATCTCCTGTGCCTGCTATTTTATTTAGACATCGAGTATCTAAAGAAATTTATTATCTGTTATTCTACATGCTTAATGTTATTATTTGATATTCTTATCTCTTGATGTTCATGTAGTAATTTGATAGTATAATCGTAATATTGGACTTCATAGTAAAGAAGCTAG
Unigene0027916	GGGTAAAAAAGAGTAAAGTTGAGATCAAATCCAACAAATCTTGCAACCCTTGTCTCTCCTCTCCTTATATTCCCTTCCTTTCTATAATTATCATCCTTTCTCCATTTCTTGAGAGAGAAGAGAGAGAGAGGGGATGGGGAGGGGAAGAGTGGAGCTGAAGAGGATTGAGAACAAGATCAACCGCCAAGTCACCTTCTCGAAACGCCGGAACGGGCTGCTGAAAAAGGCCTATGAGCTCTCGGTCCTCTGCGATGCGGAGGTCGCTCTCATCATCTTCTCCAGCAGAGGCAAGCTTTATGAGTTCGGCAGCGCAGGGACCAGTAAAACTCTTGAGCGTTACCAACGTAGTTGTTACACTTCTCAAGATACTAATGCCGTTGATCGCGAAACCCAGAGTTGGTATCAAGAAATGTCCAAATTGAAGATGAAATTTGAGTCTCTACAACGCTCCCAAAGGCATTTATTGGGAGAGGACCTCGGACCATTGAACGTGAAAGAATTACAGCAATTGGAACGCCAACTTGAATCTGCTTTGTCACAAGCTAGGCAAAGAAAGACTCAATTGATGTTGGAACAAGTGGACGAACTGCGTAAAAAGGAACGCCATCTCGGTGAAATTAACAAGCAACTAAAAAACAAGCTTGAGGCTGAGGGGTTTGACTTCCGATCTTTGCAAGTATCATGGGATACGGAGGATGTTGTTGGAGGAAATACATTTTCGCAGCATCATATGCCATCTAGTTCGATGACTATTGAACCCACTCTTCAGATGGGGTATCACCAATTTGTTCCTGCTGAAGAAGCTTCAATGCAAAGGAACACCGGTGCGGAGAATAATTTCATGCTAGGTTGGGTTCTTTGATTGAAGATTGGTTAGTACGTGTTATGTCTCGTAAAAGATCATCGGATTGTTTAACTATGTTTTCAGTTGCTTTCTTAGCTATTGTATTTCGACTTCTTTCAAATTTGCTGCTGTGGCTGATCTGCTTGTATCCGAAAGTTTTTGTTGTTGAAAATTTCATGCAGATTAATTACTGGATGGTTAGATGTATTGAATTCGGATGAATAAATTGTGTAAGGTGATGCTGTTTTTATGATGCAGCATT
Unigene0004890	AAAAGTTATGCATACCAGTACATAAACCTATACATAGATATAGTTATGCCCCCTTTCTTCGAGAGGGGTAACACCATAGCAAAACCAGATCTCTGCTGCTATAAGAATGATAGTGAAACTTAGCAGAATTCCCCGTAAAATAATTATGATGGGCGGGGAAGGTGATAGCTTATCTGTTAACGTGGCGAAGCATCCATGGCGGTAGTGAGCTGCTGCCAACACGCATCAGCGGCTGTTCAGCTGCCCCCTCGTCTGCGTCGCTGCTGCTGCTGCCTCTTTCTGGGTAGATTCCAGCGTTTTGGACGGGGTGCAAATGGGGCATTAGGAAAGGAGGGGGCCTTGTCATGGGCTGTTGTCCTTGTAGCTCCCAGTGATCTTGCTGTGTCAAAGCCTCCATCCTCTGGAACTCCATGAGCTTTTTCTCCATTATAGTGTTTTGTTCCCTCAGTGATTTCTCTGTTCGCTGGAGCTCGGTAATCGAATCAAACAATAGTTGGTGCTTCCTTGATCTTACATGCTTGAAAGATAATTCGAGCTGCTGCTCTAGTTGTTGGAGTTGTTTCAGAGTCAAGTCCTCAAGCTGCTCCCCCATGAGATGTCTTTGGCTTTTCTGCAAGGTTTCAACCTTAGCCTTCAGTCTCCCGTACTCTTGACACCAGTTTCCCTGTGCTTCAGGATATGTGTCTGTCACTGCAGTTTCTGCTTGAGAATAAAGCTCGTAACGTTCGAGAATCCTTTCCATG
Unigene0039518	AAGAAGATCGAAAACTCAACGAATCGGCAGGTCACTTACTCGAAGCGCCGGACTGGAATCATCAAGAAGGCGACTGAGCTCACTGTGCTCTGTGATGCCGAGGTCTCTCTCCTTATGTTCTCCAGCACCGGGAAGCTGTCAGAGTTCTGCAGCCCCTCCACAGACACGAAAAAGATCTTCGATCGCTACCAGCAGCTGTCTGGTATCAACCTCTGGAGCGCGCAATACGAGAAAATGCAAAACACTTTGAACCATCTGAGCGAGATCAACCGCAACCTTCGCAAGGAGATCAGCCAGAGGATGGGGGAGGAGCTGGATGGATTGGACATCAAGGACCTGCGCGGTCTTGAGCAAAATTTGGACGAAGCGCTCAAGCTCGTTCGTCACCGCAAGTATCATGTGATCAACACTCAGACAGAGACTTACAAGAAAAAGGTCAAAAACTCGGAAGAAGCACACAAGAACTTGCTCCGTGACCTGGTGAATCGAGAGATGAAAGATGAGAATCCAGTCTATGGTTATGTGGACGAAGACCCCAGCAACTATGATGGCGGCCTTGGTCTGGCCAATGGGGCTTCTCATCTGTACGAGTTCCGAGTCCAACCGAGCCAGCCAAACCTGCACGGGATGGGTTATGGCTCCCATGATCTCCGACTGGCTTGAAACCCTAATTCTGTAGGGTGCAGTGA
Unigene0040377	GAATAACGCTCATAGCGTTCCAGAATTCTTTCCATGCTAGCGTCAGTAGAGTACTCGTACAGCTTGCCCTTGGCGGAGAATACAACAAGAGCGACCTCTGCATCGCAGAGGACAGAGATCTCATGCGCCTTCTTCAGCAGCCCCGACCGCCGCTTCGAGAAGGTGACTTGCCGGTTGATCTTGTTCTCTATCCGCTTCAGCTGCACTCGCCCCCGGCCCATCTCCAGAAAACCCTAAAACTATTGAGATGGATGAGATATCCTCAGCTTATTGAAGAGTGAGGGGGAGACAGAA
Unigene0003323	CGCCAGAGGGAGTGAGTGAGACCCATAGAGGGAAATTTTGGAGGGAAAAAAATGGGGAGGAGGAAGAGGGAGATAAGGCGGATTGAGGACACCAACAGCCGCCAGGTCACTTTCTTGAAACGCGGCAGTCGGCTGCTGAAGAAGGCTTCAGAGCTCTCCGTGTTTTGCGATGCCGAGGTTGCGCTGATCGTCTTCTCACCCATGGGCAATCTCTGCGAGTTCTCCAGCTGCAGGTTAGGGTGGTATCGGTTCCAATCAAGGAGTATCCTGCTTGGCCGCCTGCAACGCGGACCAGCTGATTGGAATTTTGTTTATAATGCTGCTACTTTTAGAATAAC
Unigene0026977	AGAGCTTTCTTCTCAATTACCAAAAGTATAATATTTCCTCTTGTGGTTCTAAATACAAATATTGCTCATAGATGGAGCCTATACCTCAACTCTACTTTTAGTGTATATTTAGCATATACAAATATATTTTCTAATTCTTTTATCATTATGGTCCTTACCCTATGTTCGCATTTATCTGAACAATGCTGCCTTCATAGGCAGCAGCGGTTCCTTCATACGCAGCAGCGGAGCATGATGCATTCTCGTTCCCTGGCATATGCGAGCAGTTCGCAGATGGATCCAGCTGCTCATGTGGCACCATCGATGGCAGCTCCGCTGATGCCATTGAATGCTGGATCATCGGGTAGGGACCAGTGGGGATGAGAGACGAGAGGAGCTCAGTAGAGGTGTATGCCTGGTGGTGGTGCCACATGTCGTTTTGACTGTTTATGTGACAACCATCCATGCTGGGATCCACCTGTAGCCCTGTGTTCTGGTGGATCGGGTTGTAAATCCCTTGATCCCTGAGGGACATTAGTAGATCGGACCCAACAAACATCTGATGGCCTGAGTTGGATGTTCCCTCAGTGATCCATTGGACCATCTCGTTTCCGAACGCATTGGGGATTCCTTCTTGATGAGGTTGCATGTACATCTGCATATTAGATGTAGAGGGATCGAATGTCGACAGATGATTATTTAGCAAATATTTCTTTCTCTCGTTAACTCTTGTTAGTGTCTCAATGAGGAACTTTTCACAAGTTTGCAGCTCATCCATTGATCCCATACTCAAAGGATCCGGCTCGAAACACCTTAATTGATTTTCCGAGTGTTGTAATTGTTCTTGGTATCTACTAATCTCTTGGTGGAGCTCCTCCATGTTGGAGTTACACACCCCAGGATTGGCTATTTGGGCCGCCATGTCACTCTCGCACTTTAGCTTCTTCAAGGTTCTGATTAAGTATTCTCTATTTTGGATCACTCCTCCCCGATCGTGCTCCGGGAGGCTGATGTATCGAGCGAGAACATCCTCGATCCTTCGACGGCCGGAGAAGTGGCTGAGGCGGCCGGAGGGGGAGAACATGATTAGGGCAATGTCGATGTCGCAGAGGATGGAGAGCTCGTAGGCCTTCTTGATGAGGCCATTGCGACGCTTGGAGAAGGTGACTTGGCGATTGGTGTTGTTCTCGATCTTCTTGATTTGGAGCTTTACGCGGCCCA
Unigene0031138	AACATGAGATAGATATCAACTTCAACAGGCCTTCAGAAGGAAATATGCAGCAACTGCAGTGTGAAGCCGAGGGCCTCGCGAAGAGGATTCAAATCCTTGAAGATTATAAACGGAAGGTGATGGGTGAAAATCTGGAAACGTGTTCAATTGAAGAACTTCATGAATTAGAGGGTCAATTAGAGCAAAGTTTGAGCAAAATCAGACAAAAGAAGAATCACGAACAATCAGAGCAAATTGTGCAGTTAAAAGAACAGTTGTCTTCTTGCATTTTGCTCACAAAAATTCAGGAGAGGATTCTGTTGGAGAAGGAGAGGATTCTGTTGGAGAAGAATGCATCATTACAAGAAAAGGACACTGATTCTACAGAAGTTCTAGATGCTGACATGGAGGTACTCACGGAGCTGTTTATCGGGAGGCCTGGGTGCAGTAAAA
Unigene0025453	TTAGTTTACATACATATCGTAACACTCAAAAATGAATCCGTTACACAAATAATCGAATCTCTATTTCTCAGATATAATGTACAACCTGAGGACAATATGCTTTATCTATCATGACACTATACAACGAAAGTTTTATATACGACGTATAACAGCTGAAATAATTTGAGAATCAGTATTGTTAAAACAGGTCCTGTATTTGTATAAGAGTAGCTACATACATCCCCACTATTAGTCCAGTGCCCGTCGTTGAATTAAATGACGATACCGGCGTATTTTCATTGACTGGAGGCGAGCCTAGGGGATGAACACTGCACAGGCTCAGGTGCTGTATGATCTACTGAATCTGGTCTGGTTGTTGGGGGTAAGGATGGTTATCAAACATGGGAACAGACGTGGAAGCCCATGTCTCGATGTTGGCATCATATTCAGGCCCAGGGGGCTCAAAACCATTAACTTGGTAATCAACCCGGCCCTCCTGTAAGCTAATTTCTGCATCGGGGTTGAACTTATTCTCGCTAAGCAAATTCATTCCATACGACTGATTAGGGTAGTGGCCTCCAAGCTGCAGTTGCAAGCATGAGTTTTGGCTCAAATCTAACTGCTCTTTGGTGTCTACCTGCTTTCCCATGGTAAAGTAACCAGGAAAGCCTTGAAGAGACCCTTCTGCGGAGCACTCAATATCTCTTTGGGAAAGCAGATTAGGGCCTTCTGGAAGCATCATGTGTGAACCATTACAGTCCTGAAGCCATTGCATATGTGACAGTTGTTGCTCGCCGTTAATTGTCAGGGGTAAATGCATACCATTCTGGAACTGGCCAGAACATTCTAATGACATAATTTGCTGCTTTCCCAGGTTTTCCTTGTGCATTCTGATTCGGTTCACAGACTCTTCAAGGGATTCCTCCATTGCTCTGATATGATCTATACTGTTGATCTTATCGATATCTTTCCAATAACTTAGTCTCTTTTCAGCGTCTGATATCTGAGCTTGCAAGAACCTTGAGTGATTTGTCAATTCCTCAATTGTTTGGGTGCTTGAACCTAGAAAATCTTGTATATTGACATCATGGTCGAGCTTCTTGAATGTCTTCTTCAGTGCTTCAAGGCTCTCCAACTTCCTCTTTGCCCTCTCCTGTGGAGTTAACTGAGCAAACTTCGAAATGACCTCCTCAATATTGCTGCGATCTCCCAGGCACATTGTTGGCTTCCCAGTCGGCGAGAACATGAGAAGGATGATATCAATGTCGCACAAAATCGATAGCTCCTTGGCCTTCTTTAGGATCCCGGCTCTCCGTTTAGAGTAAGTCACCTGCCGCGCACTGGTATTCTCCAATCTCTTGATTTTCAACTTCACCCTCCCCATCTGATCACCCTTGTGCTTAAAAGGGAATCGAGATAACCCTCGAAAAACAAAGGACCCAAATGCAATCAAGGCAACTGTCTCAAAATTCGTCCGATGTGCAACCGAATCAATAGATGGTATGAAAAAATTGCGAGAACCCAGAATTCGGAAGGGAAGAAGGACGCTGATGAAGATCAAAATAGGCAATTAGAATGAAAATTGAGGGTATCCCAATTCAGAGAATTGGACAACAAGCACGGGTTGGGAGTTCCGGAGATAGAACATCAAGAACACGATCTGT
Unigene0031144	GCAATAGAGCAACCCTTGAGCGAGGGAGGGAGTGACAATCATAGAGGGAATTTTTTTGGAGGGAAAAAATGGCGAGGAGGAAGAGGGAGATAAGGCTGATTGAGGACACCAACAGCCGTCAGGTGACTTTCTCGAAGCGCGGCAGTGGGCTGCTGAAGAAGGCTTTCGAGCTCTCCGTGCTTTGCGATGTCGAGGTTGCGTTGATCGTCTTCTCACCCAAGGGCAAGCTCTGCGAGTTCTCCAGCTCCAGCATGCAAAGTACAATCGATCATTATCTGATGCATGAGAAAGATATCGACATAAGCAGGCTTTCAGAAAAAAATATGCAGCAAATGGAATGTGAATCTGCGGATCTCATGAAGAGCATTGAAATCCTTGAAGATTATAATAGGATGCGGTTGTGTGAAAATCTGGAAACATGTTCAATTGAAGAACTTCATGAAATAGAGGGTCAGTTAGAGCAAAGTTTGAGAACAATCAGACAAAAGAAGAATCACATTATATCAGAGCAAATTGCGCAGTTAAAAGAAAAGGAGAGGATTCTGTTGGAGAAGAATGCATCATTACAAGAAAAGTTCAAGGAAAAAACTCCACAACAGAAAACTGATTCTACGGAAGTTCTAGATGCTGACATGGAGGTGCTCACTGAGCTGTTTATTGGGAGGCCTGGGTGCAGCAAAACCCGAAGCATGCCAAGAGGTTGATTGAAATGCATGAGGCGCCGGGCAAGAGCCGGGAGCGGCAGATCGGACAATTCACATCGACCCCGCTTTGTCTTGACGGGGGAGGCGAACCGGACGACGGTCAGCTTTGTCTTGACGGGGGCGGCGACAGAGCGAGCGGAGGGGAAGCGGAGCACGGCGGTGATGATGGGGTTCGTGGAGGAGA
Unigene0042321	TTTGAGATCACGCAACTGATCAAGTATTATCTGAGTCTTTTTCGACCTGATCTGATTTAAAGACTTCTCTAGTTGATTGTCAAGTTGTTCGAGTTCCTTGTTACTAAGTTGACCCAAGTTCTCGCCAAGGAGATTTCTACAATTCCACGGTTAAGAGTTTCATTAGCGAAATGGTTTACAACATAGATATTGCTACTACGGATTACAGATTTG
Unigene0008053	GTGGTGGAATTGAGACACTACAGATCACAGAAGCCTTCGGTGAATTCAGGTCTGGAAAGACACAGATAGCTCACACCCTCTGCGTGTCAACACAGTTGCCGGTGAGCATGCATGGAGGCAACGGCAAGGTCGCTTACATCGACACAGAAGGCACATTCCGACCAGACCGCATTGTACCAATTGCAGAGAGATTTGGAATGGACGCGAGTGCAGTACTGGACAATATCATATATGCGCGGGCATACACCTACGAGCACCAGTACAACCTTCTTCTTGCTCTAGCTGCCAAGATGTCTGAAGAGCCTTTCCGGCT
Unigene0027070	TCAGCCTCTCATTTCGGGGGAAAGAGGAAGCCCTAGAGCGCCGCCGCGTACCGATCCTCTCAATCGCTGCTCACAGATAAATTGAAGAGCTCTTCACCAGCTACCACCTGCTCACTTTATTTAAATTGTCAAGCCCATCGTCTGGGAGTACAATGAATGCCAAGAGTTGTCTCCTACTTACCTATTCATCTGAGGTTTTGAATGGAGTACCCTTATTCTTCGCATCTAATTGCCTCCCTGTGAAGGCTGTGAATCAAGAACCTGCTGGGCATGCGTTCCATGATGCTGCGCTTAAACTTTGCGGTTTGTTCGAAGAAGATGTAGTCACAGATGATCAAAGCGAGTCATCAGATGATCGAGGTCCAGCATTTATGGCATCATCAGATTCATATAGAAGCAAAAGCAAAAAGAAATCTGCTGGTAAAAGTGACCAACAAGACCACTATGCATTGCTAGGCTTGGGCCATCTACGTTTTCTGGCCACTGAGGAGCAGATACGTAAAAGTTACCGGGAAACTGCTTTGAAGCATCATCCTGACAAACAGGCTGCCCTTCTCCTTACTGAAAAAACAGAAGCTTCAAAGCAAGCAAAGAAGGATGAGATTGAAAACCATTTCAAAGATATTCAAGAAGCTTATGAAGTTCTAATTGATCCCGTGAGAAGAAGGGTCTATGACTCAACAGATGAGTTTGATGATGAAGTGCCGAGTGACTGTGCACCACAAGACTTCTTTAAGGTGTTTGGCCCAGCATTTATGAGGAACGGGAAATGGTCTGTAGTCCAGCCAGTTCCTTCCTTGGGGGATGATAAAACTTCACTGGAAGAGGTGGACAACTTTTACGACTTTTGGTATGCATTTAAGAGTTGGAGGGAGTTCCCACATGCAGATGAGTTCGAGCTAGAGCAATCAGAGTCTCGTGATCATAAGAGATGGATGGAAAGGCAGAATGCAAAGCTGAGAGAAAAGGCGAGGAAGGAAGAGTATGCACGTGTTCGTTCCCTTATTGATAATGCTTACAAGAGGGACCCCAGACTCCTTCGGAGAAAGGAACAGGAGAAAGCAGAGAAGCAGAGGAGAAAAGAGGCAAAGTATATGGCGAAGAAATTGCAAGAAGAAGAGGCAGCTAGAGCTGTTGAGGAAGAGCGGCTCCGCAAGGAGGAGGATGAAAAAAAAGCAGCAGAGGCTGCTGTGATTAATAAGAAGATAAAGGAAAAGGAGAAGAAGCTCTTGCGCAAGGAGAAGACTCGCCTTCGCACGTTATCAGTATCACTAGTGTCAGATTCTTTGCTTGATCTAACGGAGGATGATGTGGAAAAAACATGCAACTCTTTTGGATTGGAGCAGCTTCGTCATCTCTGTGACGGCATGGAAGGTAGGGAAGGAATTGAGAGGGCTCAACTGCTCAAAGCTGCAGTCAGTGGTGATATGTTGGAGATCTCTAAGAAGGAACCTAATGATTTGAAACCAAATGGTTCCACGAATTCGGGCCCGAAAAGTAATGGTTCTGTAACTTCAGCGAAACCAGTAATTATGATGTCCAGCTACGAAAAGAAAGAGAAGCCTTGGGGTAAGGAAGAGATAGAGTTGCTGAGAAAGGGTATCCAGAAATATCAGAAGGGGACATCTCGTAGGTGGGAAGTTATTTCAGAGTATATCGGCACTGGGAGATCGGTTGAAGAAATCCTAAAAGCCACAAAGACTGTTCTTCTCCAAAAGCCTGATTCTTCTAAAGCTTTTGACTCCTTCCTTGAGAAAAGGAAGCCGGCGAAAGCCATTGTGTCACCCCTTACGACCAGACTTGAATCAGAAGGTTCCACTGTTGAGGCAGGAGATGCATCTTCTAAATCAATACCAACACCGTCTTTGAGCAGCAGCAGTCCTGAAAAGCCAGACGGTACTCCTGTTTCCCTTCCCAATGGGGTTCCTTCTGTCCCTGAACAAGACACATGGTCTGCTACCCAAGAAAGAGCTCTCATTCAGGCGCTAAAGACTTTCCCAAAGGATGTGAATCAGCGTTGGGAACGGGTTGCTGCTGCTATTCCCGGGAAAACCATGAACCAGTGCAGAAAGAAATTTTTATCAATGAAGGAGGACTTTCGAAGCAAGAAGAGTGGTTAAGTCTTTTTTCTTTTGCAGGTAAGTTCAATATAGAACTAAAAAAAGATGACCTCTTCATCATGGTGCTGAAATTTTCCTCATTACTGGGCTTAGCTGCAGTTATATCTGGGTGCTCTTTTTCCCGCAACAGAAGTTTTTGTGATGCTTTAGGATGTAGAACTAGATGGGTATGTTTATTTAAGACACAACTTTGGTGAGATCTGACAGTTGCTACTCTTTCATATGTATGAAAGTTTTGCAAGCTCCTTGATGGGAGTTGGAAGCCCAGGCTCCTGACAAACAAATGGTTGCTGAAACATCAGACTGGAGTATAATCATCAAACCTAAAGGTATATTTATTTCTTAATGATCTCGAGAAATTTATTCATGTGATGCTGTTCAAAAGAGTTGGTGTAGCTCTATGTCGGCAATTTTGCAGTGACAATTATTTAGCCATCTGATGC

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	细叶百合 L. pumilum	川百合 L. davidii	卷丹 L. lancifolium	百合 L. brownii var. viridulum
组装的总碱基 Total assembled bases	31 251 564	37 017 020	28 657 033	32 992 838
Unigene数目 Unigene number	44 565	51 413	41 638	44 716
GC含量 GC percentage(%)	48.25	47.91	47.34	47.76
N50	1 066	1 149	1 017	1 183
平均长度 Average length	701	719	688	737

	野百合 L. brownii	细叶百合 L. pumilum	川百合 L. davidii	卷丹 L. lancifolium	百合 L. brownii var. viridulum
Nr	28 104	28 681	31 641	26 438	29 932
Swiss-prot	17 739	18 283	20 390	17 381	19 951
GO	6 494	6 785	7 398	6 475	7 360
KOG	14 682	14 958	16 619	14 371	16 268
KEGG	11 984	12 557	13 570	11 960	13 247
所有注释的基因数目 All annotation unigenes	28 187	28 709	31 827	26 527	30 025
注释基因的百分比 The percentage of annotated unigenes(%)	68.89	55.23	61.54	56.96	48.93

基因ID Gene ID	基因 Gene	蛋白质 Protein
Unigene0017918	MADS2	MADS盒转录因子2 MADS-box transcription factor 2
Unigene0027916	MADS6	MADS盒转录因子6 MADS-box transcription factor 6
Unigene0004890	MADS14	MADS盒转录因子14 MADS-box transcription factor 14
Unigene0039518	MADS16	MADS盒转录因子16 MADS-box transcription factor 16
Unigene0040377	AGL8	无性MADS盒蛋白AGL8 Agamous-like MADS-box protein AGL8
Unigene0003323	AGL15	无性MADS盒蛋白AGL15 Agamous-like MADS-box protein AGL15
Unigene0026977	AGL104	无性MADS盒蛋白AGL104 Agamous-like MADS-box protein AGL104
Unigene0031138	AGL19	无性MADS盒蛋白AGL19 Agamous-like MADS-box protein AGL19
Unigene0025453	AGL30	无性MADS盒蛋白AGL30 Agamous-like MADS-box protein AGL30
Unigene0031144	SOC1	MADS盒蛋白SOC1 MADS-box protein SOC1
Unigene0042321	EJ2	MADS盒蛋白EJ2 MADS-box protein EJ2
Unigene0008053	LIM15	减数分裂重组蛋白DMC1同源体 Meiotic recombination protein DMC1 homolog
Unigene0027070	GlsA	无性生殖细胞形成蛋白GlsA Gonidia forming protein GlsA

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百合属5种植物的比较转录组分析

Comparative Transcriptome Analysis of Five Species of Lilium

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