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植物研究 ›› 2021, Vol. 41 ›› Issue (4): 614-625.doi: 10.7525/j.issn.1673-5102.2021.04.017

• 研究报告 • 上一篇    

百合属5种植物的比较转录组分析

赵雪艳, 李思锋, 柏国清, 李为民()   

  1. 陕西省西安植物园,陕西省植物研究所,陕西省植物资源保护与利用工程技术研究中心,西安 710061
  • 收稿日期:2020-08-29 出版日期:2021-07-20 发布日期:2021-03-24
  • 通讯作者: 李为民 E-mail:763730163@qq.com
  • 作者简介:赵雪艳(1986—),女,讲师,博士研究生,主要从事植物的解剖结构以及资源开发利用方面的研究。
  • 基金资助:
    陕西省重点研发计划项目—农业领域(2018NY-88);陕西省科学院科技计划项目(2020K-28)

Comparative Transcriptome Analysis of Five Species of Lilium

Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI()   

  1. Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi’an Botanical Garden of Shaanxi Province,Institute of Botany of Shaanxi Province,Xi’an 710061
  • Received:2020-08-29 Online:2021-07-20 Published:2021-03-24
  • Contact: Wei-Min LI E-mail:763730163@qq.com
  • About author:ZHAO Xue-Yan(1986—),female,lecturer,doctor,Research interests: anatomical structure of plants and resource exploitation and utilization.
  • Supported by:
    Key technology R&D program of Shaanxi province(2018NY-88);Science and technology program of Shaanxi academy of sciences(2020K-28)

摘要:

百合属植物是多年生的宿根草本植物,具有重要的观赏价值,有的种可食用也可药用。本研究对百合属4种植物(细叶百合、川百合、卷丹和百合)花的转录组进行测序,并结合前期的野百合的转录组数据进行比较转录组分析,鉴别花发育相关基因。并筛选出5种植物的直系同源基因,进而检测正选择基因,分析它们对环境的适应性。通过组装分别在细叶百合、川百合、卷丹以及百合花中获得了44 565、51 413、41 638和 44 716个unigene。为了了解unigene功能信息,将其在公共数据库中进行比对和注释,发现了13个花发育相关基因。另外,在5个物种间共获得8 247对直系同源基因,并计算其Ka、Ks及Ka/Ks值。在5个物种中有33对直系同源基因受到了强烈的正选择作用,随后对其进行GO、KEGG功能富集分析及基因功能注释,发现这些基因主要是与抗性以及生物合成相关的基因。

关键词: 百合属, 转录组, 花发育基因, 直系同源基因, 正选择基因

Abstract:

Lilium are perennial herbaceous plants with important ornamental value and some species are edible and medicinal. In the present study, the transcriptome of four species of LiliumL. pumilumL. davidiiL. lancifolium and L. brownii var. viridulum) was sequenced respectively, and the genes which involved in flower development were analyzed. Combined with the previous transcriptome data of L. brownii, the orthologous genes were selected by comparative transcriptome analysis. Positive selections were screened and their environmental adaptability were analyzed respectively. 44 565, 51 413, 41 638 and 44 716 unigenes were obtained from L. pumilumL. davidiiL. lancifolium and L. brownii var. viridulum respectively by assembly. In order to understand the function of unigene, the unigenes were compared and annotated in the public database. 13 genes related to the development of flower were identified. A total of 8 247 orthologous genes were obtained in five species, and Ka, Ks and Ka/Ks values were calculated. 33 pairs of homologous genes were strongly positive selected in five species. Subsequently, GO and KEGG enrichment analysis and gene function annotation were carried out, and it was found that these genes were mainly related to plant defense and biosynthesis.

Key words: Lilium, transcriptome, flower development gene, orthologous gene, positive gene

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