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植物研究 ›› 2020, Vol. 40 ›› Issue (4): 602-612.doi: 10.7525/j.issn.1673-5102.2020.04.015

• 研究报告 • 上一篇    

日本落叶松LoERF017基因的克隆及生物信息学分析

马苗苗, 李成浩, 刘晓, 张馨, 杨静莉   

  1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040
  • 收稿日期:2019-11-18 发布日期:2020-06-12
  • 通讯作者: 杨静莉,E-mail:yifan85831647@163.com E-mail:yifan85831647@163.com
  • 作者简介:马苗苗(1996-),女,硕士研究生,主要从事分子生物学研究。
  • 基金资助:
    国家转基因生物新品种培育重大专项(2018ZX08020-003)

Cloning and Bioinformatics Analysis of LoERF017 Gene from Larix kaempferi

MA Miao-Miao, LI Cheng-Hao, LIU Xiao, ZHANG Xin, YANG Jing-Li   

  1. State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-11-18 Published:2020-06-12
  • Supported by:
    Genetically Modified Organisms Breeding Major Project of China(2018ZX08020-003)

摘要: 从日本落叶松中克隆得到的LoERF017基因,该基因全长624 bp,共编码206个氨基酸。预测该基因所编码的蛋白只包括一个AP2结构域,其二级结构包括α-螺旋(30.43%),无规卷曲(54.11%),延伸链(12.56%),β-折叠(2.9%)。对该基因进行亚细胞定位,结果显示LoERF017基因定位在细胞核中。荧光定量PCR结果表明,LoERF017基因在日本落叶松中的表达有一定的组织特异性,该基因在根中的表达量最高,在茎中的表达量最低,在叶中的表达量居中。干旱胁迫下,LoERF017基因的表达量在根中呈上升趋势,复水后,该基因的表达量有下降趋势。可以初步预测该基因主要在日本落叶松根中表达并行使功能,并推测该基因可能与抗旱相关。

关键词: 日本落叶松, LoERF017基因, 生物信息学, 非生物胁迫

Abstract: The LoERF017 gene cloned from Larix kaempferi was sequenced and found to be 624 bp in length, encoding a total of 207 amino acids. The protein encoded by LoERF017 is predicted to include only one AP2 domain, and its secondary structure includes α-helix (30.43%), random coil (54.11%), extended strand 12.56%), and β-sheet (2.9%). Subcellular localization of the gene showed that LoERF017 was located in the nucleus. The results of qRT-PCR showed that the expression level of LoERF017 gene in L.kaempferi had certain tissue specificity. The expression of this gene in root was the highest, the expression in stem was the lowest, and the expression in leaves was middle. Under drought stress, the expression of LoERF017 gene increased in the root, but decreased after rehydration. It was preliminarily predicted that this gene is mainly expressed and functions in root of L.kaempferi, and it was speculated that LoERF017 might be related to drought resistance.

Key words: Larix kaempferi, LoERF017, bioinformatics, abiotic stress

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