不同喀斯特小生境中小蓬竹的遗传多样性

doi:10.7525/j.issn.1673-5102.2019.01.009

摘要/Abstract

摘要： 通过RAPD标记法对5类喀斯特小生境中小蓬竹的遗传结构及其多样性进行了分析。RAPD随机引物8个扩增共得87个清晰度高且重复性好的条带，分子量200~3 000 bp，含多态68条，物种PPL=78.16%，Nei's基因多度H=0.291 3，Shannon多样性指数I=0.431 0。土面（TM）生长的小蓬竹遗传多样性水平最高（PPL=47.13%，H=0.195 3，I=0.283 4），石缝（SF）中的小蓬竹遗传多样性水平最低（PPL=39.08%，H=0.147 4，I=0.219 2）。POPGENE 32分析5类小生境中小蓬竹的遗传分化系数G_st=0.415 1，基因流N_m=0.704 5。5类小生境的小蓬竹间遗传相似系数（I）变化范围0.777 3~0.999 4，平均为0.865 6；遗传距离（D）变化范围0.000 6~0.251 9，平均为0.148 3。各类小生境的遗传多样性水平PPL均值为43.91%，5类小生境间的遗传分化G_st=0.405 4。研究结果说明，小生境间的差异增加了小蓬竹的遗传多样性。

关键词: 小蓬竹, RAPD-PCR, 遗传多样性, 小生境, 濒危机制, 保护策略

Abstract: By random amplified polymorphic DNA(RAPD) markers analysis, 5 kinds of karst microhabitats of Ampelocalamus luodianensis was conducted to study genetic diversity and genetic structure. Through 8 RAPD primers, 87 discernible DNA fragments in all weighted from 200-3 000 bp were cloned, and 68 in those were polymorphic loci, with percentage of polymorphic loci at the species level, PPL=78.16%, Nei's index H=0.291 3, and Shannon's Information Index I=0.431 0. The A.luodianensis grew on soil surface(TM) possessed the highest level of genetic diversity(PPL=47.13%, H=0.195 3 and I=0.283 4), while the A.luodianensis in stone crevice exhibited the lowest levels of genetic diversity(PPL=39.08%, H=0.147 4 and I=0.219 2). Coefficient of genetic differentiation (G_st) was 0.415 1(from the POPGENE32), and the gene flows(N_m) was 0.704 5. Among the 5 microhabitats, genetic identity varied from 0.777 3-0.999 4, genetic distance was different from 0.000 6-0.251 9, and the mean of them were 0.865 6-0.148 3 severally. Genetic diversity in niches at mean value was with PPL of 43.91%. Coefficient of genetic variation in niches was with G_st of 0.4054. The results indicated that differences of microhabitats increased the genetic diversity of A.luodianensis.

Key words: Ampelocalamus luodianensis, RAPD-PCR, genetic diversity, microhabitat, endangered mechanism, conservation strategies

中图分类号:

S795.9

刘济明, 管睿婷, 王敏, 陈敬忠, 童炳丽, 武梦瑶. 不同喀斯特小生境中小蓬竹的遗传多样性[J]. 植物研究, 2019, 39(1): 69-77.

LIU Ji-Ming, GUAN Rui-Ting, WANG Min, CHEN Jing-Zhong, TONG Bing-Li, WU Meng-Yao. Genetic Diversity of Ampelocalamus luodianensis in Different Microhabitats[J]. Bulletin of Botanical Research, 2019, 39(1): 69-77.

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