NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响

doi:10.7525/j.issn.1673-5102.2018.05.005

植物研究 ›› 2018, Vol. 38 ›› Issue (5): 669-674.doi: 10.7525/j.issn.1673-5102.2018.05.005

NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响

赵晓菊^1,2, 张丽霞¹, 满秀玲²

1. 大庆师范学院生物工程学院, 大庆 163712;
2. 东北林业大学林学院, 哈尔滨 150040

收稿日期:2018-04-08 出版日期:2018-09-15 发布日期:2018-08-31
通讯作者: 满秀玲 E-mail:mannefu@163.com
作者简介:赵晓菊(1978-),女,副教授,主要从事植物生理生态研究。
基金资助:
黑龙江省青年科学基金（QC2015042）

Effects of Exogenous NO on Seed Germination and Physiological Metabolism in Catharanthus roseus Seedling under NaCl Stress

ZHAO Xiao-Ju^1,2, ZHANG Li-Xia¹, MAN Xiu-Ling²

1. Bioengineering Institute, Daqing Normal University, Daqing 163712;
2. College of Forestry, Northeast Forestry University, Harbin 150040

Received:2018-04-08 Online:2018-09-15 Published:2018-08-31
Supported by:
This study was financially supported by Natural Science Foundation of Heilongjiang province(QC2015042)

摘要/Abstract

摘要： 为探讨NO对盐胁迫下长春花（Catharanthus roseus）种子萌发及幼苗生长的缓解作用，在50 mmol·L^-1 NaCl胁迫下，研究了不同浓度外源NO供体硝普钠（SNP）处理对长春花种子萌发及幼苗生理代谢的影响。研究表明：盐胁迫抑制长春花种子萌发，0.1 mmol·L^-1 SNP缓解盐胁迫对长春花种子萌发和幼苗生长效果最好，显著提高种子发芽率、发芽指数、活力指数和叶片中脯氨酸（Pro）含量，降低了丙二醛（MDA）含量，0.1~0.5 mmol·L^-1 SNP下硝酸还原酶（NR）活性增强，大于0.5 mmol·L^-1 SNP加重盐胁迫伤害。适当浓度的NO可有效缓解盐胁迫对长春花种子和幼苗的伤害。

关键词: 一氧化氮, 盐胁迫, 长春花, 种子萌发, 硝酸还原酶

Abstract: Under 50 mmol·L^-1 NaCl stress condition, we studied the relaxation effects of sodium nitroprusside(SNP, an exogenous nitric oxide donor) at the contents of 0.05, 0.1, 0.5, 1.0 and 2.0 mmol·L^-1 on germination and physiological metabolism in Catharanthus roseus seedlings. The results showed that NaCl stress inhibited the germination of C.roseus and SNP could alleviate its stress effect. At the 0.1 mmol·L^-1 of SNP the seed germination, germination and vitality index, and contents of proline in leaves were increased significantly, while malondialdehyde(MDA) content in leaves were decreased dramatically. Nitrate reductase(NR) activities in 0.1-0.5 mmol·L^-1 SNP groups reached to higher levels compared to that of control group. However, more than 0.5 mmol·L^-1 SNP enhanced the stress effect of NaCl. Therefore, the 0.1 mmol·L^-1 was the most appropriate content for SNP to alleviate NaCl stress. In conclusion, appropriate contents of exogenous NO could alleviate NaCl stress on C.roseus seeds and seedlings effectively.

Key words: Nitric oxide, salt stress, Catharanthus roseus, seed germination, nitrate reductase

中图分类号:

Q945.78

赵晓菊, 张丽霞, 满秀玲. NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响[J]. 植物研究, 2018, 38(5): 669-674.

ZHAO Xiao-Ju, ZHANG Li-Xia, MAN Xiu-Ling. Effects of Exogenous NO on Seed Germination and Physiological Metabolism in Catharanthus roseus Seedling under NaCl Stress[J]. Bulletin of Botanical Research, 2018, 38(5): 669-674.

参考文献

1. Parida A K,Das A B. Salt tolerance and salinity effects on plants:a review[J]. Ecotoxicology and Environmental Safety,2005,60(3):324-349.
2. 刘建新,胡浩斌,王鑫. 外源NO对盐胁迫下黑麦草幼苗活性氧代谢、多胺含量和光合作用的影响[J]. 植物研究,2009,29(3):313-319.Liu J X,Hu H B,Wang X. Effects of exogenous nitric oxide on active oxygen metabolism,polyamine content and photosynthesis of ryegrass(Lolium perenne L.) seedlings under salt stress[J]. Bulletin of Botanical Research,2009,29(3):313-319.
3. Kumar V A,Kumar S C,Seema S. Catharanthus roseus and purposeful exploitation of saline land[J]. Bhartiya Krishi Anusandhan Patrika,2013,28(2):94-95.
4. Liu J,Liu Y,Wang Y,et al. The combined effects of ethylene and MeJA on metabolic profiling of phenolic compounds in Catharanthus roseus revealed by metabolomics analysis[J]. Frontiers in Physiology,2016,7:217.
5. Zhao X J,Pan Y J,Chen H F,et al. Influence of holoparasitic plant Cuscuta japonica on growth and alkaloid content of its host shrub Catharanthus roseus:a field experiment[J]. Arabian Journal for Science and Engineering,2018,43(1):93-100.
6. Tang Z H,Liu Y J,Guo X R,et al. The combined effects of salinity and nitrogen forms on Catharanthus roseus:the role of internal ammonium and free amino acids during salt stress[J]. Journal of Plant Nutrition and Soil Science,2011,174(1):135-144.
7. Wendehenne D,Pugin A,Klessig D F,et al. Nitric oxide:comparative synthesis and signaling in animal and plant cells[J]. Trends in Plant Science,2001,6(4):177-183.
8. Siddiqui M H,Al-Whaibi M H,Basalah M O. Role of nitric oxide in tolerance of plants to abiotic stress[J]. Protoplasma,2011,248(3):447-455.
9. Li X L,Pan Y J,Chang B W,et al. NO promotes seed germination and seedling growth under high salt may depend on EIN3 protein in Arabidopsis[J]. Frontiers in Plant Science,2016,6:1203.
10. Hung K T,Chang C J,Kao C H. Paraquat toxicity is reduced by nitric oxide in rice leaves[J]. Journal of Plant Physiology,2002,159(2):159-166.
11. He J Y,Ren Y F,Chen X L,et al. Protective roles of nitric oxide on seed germination and seedling growth of rice(Oryza sativa L.) under cadmium stress[J]. Ecotoxicology and Environmental Safety,2014,108:114-119.
12. Duan P,Ding F,Wang F,et al. Priming of seeds with nitric oxide donor sodium nitroprusside(SNP) alleviates the inhibition on wheat seed germination by salt stress[J]. Journal of Plant Physiology and Molecular Biology,2007,33(3):244-250.
13. Uchida A,Jagendorf A T,Hibino T,et al. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J]. Plant Science,2002,163(3):515-523.
14. Shi Q H,Ding F,Wang X F,et al. Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress[J]. Plant Physiology and Biochemistry,2007,45(8):542-550.
15. 张志良,瞿伟菁. 植物生理学实验指导[M]. 北京:高等教育出版社,2007:41-277.Zhang Z L,Qu W J. The experimental guide for plant physiology[M]. Beijing:Higher Education Press,2007:41-277.
16. Baysal G,Tipirdamaz R. The effect of salinity on lipid peroxidation and some antioxidant enzyme activities in two cucumber cultivars[J]. Acta Horticulturae,2007,729:199-203.
17. Delledonne M,Xia Y J,Dixon R A,et al. Nitric oxide functions as a signal in plant disease resistance[J]. Nature,1998,394(6693):585-888.
18. 王文果,陈志新,张克中,等. 一氧化氮对NaCl胁迫下冰叶日中花种子萌发的影响[J]. 北京农学院学报,2016,31(4):81-85.Wang W G,Chen Z X,Zhang K Z,et al. The effect of nitric oxide on the seed germination under NaCl stress in Mesembryanthemum crystallinum L.[J]. Journal of Beijing University of Agriculture,2016,31(4):81-85.
19. Averill B A. Dissimilatory nitrite and nitric oxide reductases[J]. Chemical Reviews,2017,96(7):2951-2964.
20. 杨少辉,季静,王罡,等. 盐胁迫对植物影响的研究进展[J]. 分子植物育种,2006,4(Z1):139-142.Yang S H,Ji J,Wang G,et al. Effects of salt stress on plants[J]. Molecular Plant Breeding,2006,4(Z1):139-142.
21. 樊怀福,郭世荣,焦彦生,等. 外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响[J]. 生态学报,2007,27(2):546-553.Fan H F,Guo S R,Jiao Y S,et al. The effects of exogenous nitric oxide on growth,active oxygen metabolism and photosynthetic characteristics in cucumber seedlings under NaCl stress[J]. Acta Ecologica Sinica,2007,27(2):546-553.
22. 马向丽,魏小红,龙瑞军,等. 外源一氧化氮提高一年生黑麦草抗冷性机制[J]. 生态学报,2005,25(6):1269-1274.Ma X L,Wei X H,Long R J,et al. Studies on mechanism of enhancing the chilling resistance of annual ryegrass by exogenous nitric oxide[J]. Acta Ecologica Sinica,2005,25(6):1269-1274.
23. 吴雪霞,朱月林,朱为民,等. 外源一氧化氮对NaCl胁迫下番茄幼苗生理影响[J]. 中国农业科学,2006,39(3):575-581.Wu X X,Zhu Y L,Zhu W M,et al. Physiological effects of exogenous nitric oxide in tomato seedlings under NaCl stress[J]. Scientia Agricultura Sinica,2006,39(3):575-581.
24. Garciía-Mata C,Lamattina L. Nitric Oxide induces stomatal closure and enhances the adaptive plant responses against drought stress[J]. Plant Physiology,2001,126(3):1196-1204.

[1]	隋德宗, 王保松. 盐胁迫下乌桕无性系叶片的比较蛋白组学研究[J]. 植物研究, 2023, 43(5): 679-689.
[2]	徐磊, 胥晓, 刘沁松. 外源水杨酸对盐胁迫下珙桐幼苗抗氧化系统和基因表达的影响[J]. 植物研究, 2023, 43(4): 572-581.
[3]	矫春晶, 李明月, 张鹏. 外源激素浸种与渗透处理对水曲柳种子热休眠的作用[J]. 植物研究, 2023, 43(3): 370-378.
[4]	唐莹莹, 郭传超, 石荡, 蒋南林, 许正, 刘立强. 果肉和埋土深度对新疆野杏种子萌发与幼苗生长的影响[J]. 植物研究, 2023, 43(2): 251-260.
[5]	廖诗贤, 王宇婷, 董立本, 顾咏梅, 贾丰璘, 姜廷波, 周博如. 小黑杨转录因子PsnbZIP1应答盐胁迫功能分析[J]. 植物研究, 2023, 43(2): 288-299.
[6]	刘森尧, 贾丰璘, 国庆, 樊高锋, 周博如, 姜廷波. 小黑杨转录因子PsnbHLH162基因在盐和低温胁迫下应答分析[J]. 植物研究, 2023, 43(2): 300-310.
[7]	刘英, 吴嘉仪, 金玲, 季倩如, 付玉杰, 李德文. 遮光胁迫下外源NO对盆栽长春花土壤养分含量及幼苗生长特征的影响[J]. 植物研究, 2022, 42(6): 1088-1095.
[8]	岳莉然, 刘颖婕, 刘晨旭, 周蕴薇. 响应盐胁迫调控的露地菊miR398a的克隆及功能研究[J]. 植物研究, 2022, 42(6): 986-996.
[9]	李登高, 林睿, 穆青慧, 周娜, 张焱如, 白薇. 马铃薯StNPR4基因的克隆与功能分析[J]. 植物研究, 2022, 42(5): 821-829.
[10]	王欢, 徐云飞, 刘一伯, 刘沁松, 徐文娟, 龙芸, 胥晓. 珙桐—灯台树枝和叶的水提物对白菜种子萌发和幼苗生长的化感效应[J]. 植物研究, 2022, 42(5): 866-875.
[11]	陈娇娆, 续旭, 胡章立, 杨爽. 植物感受盐胁迫及相关钙信号的研究进展[J]. 植物研究, 2022, 42(4): 713-720.
[12]	程赫, 田双慧, 张洋, 刘聪, 夏德安, 魏志刚. 毛果杨nsLTP基因家族全基因组水平鉴定及其表达特性分析[J]. 植物研究, 2022, 42(3): 412-423.
[13]	刘建新, 刘瑞瑞, 刘秀丽, 贾海燕, 卜婷, 李娜. 外源H₂S供体NaHS浸种对盐碱胁迫下裸燕麦种子萌发特性的影响[J]. 植物研究, 2021, 41(6): 870-877.
[14]	田双慧, 程赫, 张洋, 刘聪, 夏德安, 魏志刚. 毛果杨类胡萝卜素裂解双加氧酶基因家族全基因组水平鉴定及其干旱与盐胁迫响应分析[J]. 植物研究, 2021, 41(6): 993-1005.
[15]	曹丹, 王世震, 韩梅. 绿色环保肥料增效剂聚天冬氨酸对杨树幼苗生长的影响[J]. 植物研究, 2021, 41(5): 712-720.

NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响

Effects of Exogenous NO on Seed Germination and Physiological Metabolism in Catharanthus roseus Seedling under NaCl Stress

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价