光照强度对成熟红颜草莓果实着色和花青素生物合成的影响及可能的分子机制

doi:10.7525/j.issn.1673-5102.2018.05.004

摘要/Abstract

摘要： 为了研究光照对红颜草莓（Fragaria×ananassa Duch.‘Benihoppe’）果实着色、花青素含量及花青素合成相关基因表达的影响，本文采用高效液相色谱法（HPLC）和实时荧光定量PCR技术测定了不同遮阴处理下（透光率100%、75%、25%）草莓果实花青素含量及色素相关基因的表达情况。结果显示，与100%透光率下的草莓果实相比，在75%和25%透光率下合成的花青素含量分别下降了41.58%和92.54%；和花青素合成相关基因的表达也都有不同程度的下降，其中二氢黄酮醇4-还原酶基因（FaDFR）、类黄酮-3'羟化酶基因（FaF3'H）和类黄酮3-O-糖基转移酶基因（FaUFGT）的表达与花青素含量相关性达到显著水平；此外，在遮光条件下转录因子FaMYB10、FaMYB1表达也明显下调。可见，光照是影响草莓果实着色的关键环境因素，遮光抑制色素相关功能基因和转录因子的表达，阻碍了果实中花青素的合成，最终导致果实着色差异。

关键词: 遮光, 草莓, 花青素, 高效液相, 荧光定量PCR, 基因表达

Abstract: In order to investigate the effect of different light intensities on the fruit coloration, the content of anthocyanin, and the relative xpression of anthocyanin biosynthesis related genes in Fragaria×ananassa Duch. ‘Benihoppe’, we determined the content of anthocyanin using HPLC and analyzed the relative expression of anthocyanin biosynthesis related genes using quantitative real-time PCR under the light transmittance of 100%, 75% and 25%. The results showed that the content of strawberry under light transmittance 75% and 25% decreased 41.58% and 92.54%, respectively, compared with the control. The relative expression of anthocyanin biosynthesis related genes and transcription factors, FaMYB10 and FaMYB1, were also decreased. The expression of FaDFR, FaF3'H and FaUFGT were significantly correlated with the anthocyanin content. These results indicated that light was the key environmental factor affecting fruit coloring, and shading could inhibited the expression of anthocyanin biosynthesis related genes and transcription factors, blocked the anthocyanin synthesis in fruits, which finally contributed to the fruits coloring differences.

Key words: Shading, Fragaria×ananassa Duch., anthocyanin, HPLC, real-time PCR, gene expression

中图分类号:

S668.4

邵婉璐, 李月灵, 高松, 李钧敏, 梁宗锁. 光照强度对成熟红颜草莓果实着色和花青素生物合成的影响及可能的分子机制[J]. 植物研究, 2018, 38(5): 661-668.

SHAO Wan-Lu, LI Yue-Ling, GAO Song, LI Jun-Min, LIANG Zong-Suo. Effects of Light Intensity on the Fruit Coloration and Anthocyanian Biosynthesis in Fragaria×ananassa Duch. ‘Benihoppe’ and the Possible Molecular Mechanism[J]. Bulletin of Botanical Research, 2018, 38(5): 661-668.

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