小黑杨HD-Zip转录因子家族生物信息学及应答盐胁迫分析

doi:10.7525/j.issn.1673-5102.2017.05.011

植物研究 ›› 2017, Vol. 37 ›› Issue (5): 715-721.doi: 10.7525/j.issn.1673-5102.2017.05.011

小黑杨HD-Zip转录因子家族生物信息学及应答盐胁迫分析

张雪梅, 姚文静, 赵凯, 姜廷波, 周博如

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2017-07-14 出版日期:2017-09-15 发布日期:2017-09-15
通讯作者: 周博如,E-mail:boruzhou@yahoo.com E-mail:boruzhou@yahoo.com
作者简介:张雪梅(1991-),女,硕士研究生,主要从事林木抗逆机理方面研究。
基金资助:
国家863课题资助（2013AA102701）

Bioinformatics and Response to Salt Stress Analysis of the HD-Zip Transcription Factor Family in Populus simonii×P.nigra

ZHANG Xue-Mei, YAO Wen-Jing, ZHAO Kai, JIANG Ting-Bo, ZHOU Bo-Ru

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2017-07-14 Online:2017-09-15 Published:2017-09-15
Supported by:
National 863 project(2013AA102701)

摘要/Abstract

摘要： HD-Zip转录因子基因是植物中特有的一类蛋白家族，在植物生长发育和逆境应答胁迫过程中发挥重要作用。HD-Zip转录因子基因是由高度保守的同源异型结构域（HD）和亮氨酸拉链域（LZ）结构域构成的特殊结构模型。杨树HD-Zip转录因子家族共有63个基因，可被分为HD-ZipⅠ、HD-ZipⅡ、HD-ZipⅢ和HD-ZipⅣ四个亚家族。本文利用RNA-Seq分析了盐胁迫条件下HD-Zip基因家族在小黑杨根、茎、叶等不同组织的基因表达差异，从转录组水平揭示其应答胁迫环境的分子机制，结果表明，盐胁迫下在叶中有25个HD-Zip基因下调表达，21个基因上调表达；茎中有42个基因下调表达，11个基因上调表达；根中有26个基因下调表达，24个基因上调表达。另外，本文根据拟南芥HD-Zip转录因子家族基因的已知功能，预测了杨树HD-Zip转录因子同源基因的功能，并利用生物信息学方法分析了杨树HD-Zip转录因子蛋白序列的保守结构域、氨基酸组成和理化性质等，为进一步研究杨树HD-Zip转录因子基因功能提供参考。

关键词: HD-Zip转录因子, 非生物胁迫, 生长发育, 小黑杨, 生物信息学

Abstract: The HD-Zip transcription factor family is a kind of special protein in plant, playing crucial roles in plant development processes and abiotic stresses response. The protein family includes a highly conserved homeodomain(HD) and a leucine zipper domain(LZ), forming a special structural model. The HD-Zip transcription factor family, including 63 members, can be classified into 4 subfamilies HD-Zip Ⅰ, HD-Zip Ⅱ, HD-Zip Ⅲ and HD-Zip Ⅳ. RNA-Seq analysis of poplar roots, stems and leaves under salt stress was conducted for the differentially expressed genes in different tissues, and reveal the molecular mechanism of the response to stress environment. Therewere 25 HD-Zip genes down-regulated and 21 HD-Zip genes up-regulated in leaves under salt treatment, 42 genes down-regulated and 11 genes up-regulated in stems, and 26 genes down-regulated and 24 genes up-regulated in roots. According to gene annotations in Arabidopsis thaliana, the functions of HD-Zip transcription factor in poplarwere predicted.The conserved domains, structural characteristics and chemical properties of HD-Zip family members were also studied by bioinformatics, providing reference for gene functions of HD-Zip family in poplar.

Key words: HD-Zip transcription factor, abiotic stresses, growth and development, Populus simonii×P.nigra, bioinformatics

中图分类号:

S722.3+6

张雪梅, 姚文静, 赵凯, 姜廷波, 周博如. 小黑杨HD-Zip转录因子家族生物信息学及应答盐胁迫分析[J]. 植物研究, 2017, 37(5): 715-721.

ZHANG Xue-Mei, YAO Wen-Jing, ZHAO Kai, JIANG Ting-Bo, ZHOU Bo-Ru. Bioinformatics and Response to Salt Stress Analysis of the HD-Zip Transcription Factor Family in Populus simonii×P.nigra[J]. Bulletin of Botanical Research, 2017, 37(5): 715-721.

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小黑杨HD-Zip转录因子家族生物信息学及应答盐胁迫分析

Bioinformatics and Response to Salt Stress Analysis of the HD-Zip Transcription Factor Family in Populus simonii×P.nigra

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