武夷山地伯乐树种群遗传多样性的ISSR分析

doi:10.7525/j.issn.1673-5102.2015.04.014

摘要/Abstract

摘要： 伯乐树（Bretschneidera sinensis Hemsl.）为我国特有单型科珍稀濒危植物，具重要的科研价值。本研究采用ISSR分子标记对武夷山脉分布的5个伯乐树天然种群和1个移栽种群进行遗传多样性分析。结果表明伯乐树物种水平遗传多样性较高（PPB：75.70%；H_ES：0.304 5；H：0.450 1），种群水平则较低（PPB：60.13%；H_EP：0.238 1；H：0.347 5），MJY种群和BSZ种群分别是所有种群中遗传多样性最高和最低的。5个地理种群间遗传分化程度较高（G_st=0.218 1），原因可能源于伯乐树的繁殖方式及生境片段化，Mantel检验也证实了地理距离与遗传距离具有显著相关性（r=0.626 7，P＜0.05）。针对伯乐树种群遗传多样性现状，建议加强现有自然种群的就地保护，促进种群自然更新。

关键词: 伯乐树, ISSR, 遗传多样性, 遗传结构

Abstract: Bretschneidera sinensis Hemsl., with important research value, is an endangered species endemic in East and South Chin. We used inter-simple sequence repeat(ISSR) markers studied the genetic diversity within and among six populations of B.sinensis. The genetic diversity at species level was high(PPB: 75.70%; H_ES: 0.304 5; H: 0.450 1). However, relatively low genetic diversity existed within populations. Population in Minjiangyuan(MJY) exhibited the greatest level of variability(PPB: 60.13%; H_EP: 0.238 1; H: 0.347 5), whereas the population in Baishanzu(BSZ) showed its own variability at the lowest level(PPB: 44.86%; H_E: 0.173 6; H: 0.255 6). By the genetic analysis of the ISSRs, we revealed a relatively high level of genetic differentiation among populations by Nei’s gene diversity statistics(21.81%), Shannon’s information measure(22.79%) and analysis of molecular variance(AMOVA)(18.62%). The main factors responsible for the relatively high level of differentiation among populations were probably related to the predominant outcrossing reproductive system and recent habitat fragmentation, and a significant correlation was between genetic distance and geographic distance(r=0.626 7, P<0.05). The high diversity would hold promise for conservation and restoration efforts to save the endangered species from extinction.

Key words: Bretschneidera sinensis, inter-simple sequence repeats(ISSR), genetic diversity, genetic structure

中图分类号:

林乐静1,2；金楚齐1；郑春豪3；周亚美1；周盈1. 武夷山地伯乐树种群遗传多样性的ISSR分析[J]. 植物研究, 2015, 35(4): 559-563.

LIN Le-Jing1,2；JIN Chu-Qi1；ZHENG Chun-Hao3；ZHOU Ya-Mei1；ZHOU Ying1. ISSR Analysis on the Genetic Diversity of Relic Plant Bretschneidera sinensis from Wuyishan Region[J]. Bulletin of Botanical Research, 2015, 35(4): 559-563.

参考文献

1.Reed D H,Frankham R.Correlation between fitness andgenetic diversity［J］.Conservation Biology,2003,17(1):230-237.

2.Schaal B A,Hayworth D A,Olsen K M,et al.Phylogeographic studies in plants:problems and prospects［J］.Molecular Ecology,1998,7(4):465-474.

3.金则新,李钧敏,丁丽亚.短柄袍种群遗传多样性的ISSR分析［J］.植物研究,2007,27(5):559-564.

4.Altizer S,Harvell D,Friedle E.Rapid evolutionary dynamics and disease threats to biodiversity［J］.Trends in Ecology & Evolution,2003,18(11):589-596.

5.Reusch T B H,Ehlers A,Hammerli A,et al.Ecosystem recovery after climatic extremes enhanced by genetic diversity［J］.Proceedings of the National Academy of Sciences,USA,2005,102(8):2826-2831.

6.Soulé M E.What do we really know about extinction?［C］.//Genetics and Conservation:A Reference for Managing Wild Animal and Plant Populations(Ayala F J).Sunderland:Sinauer Associates,1983:60-77.

7.Moran P.Current conservation genetics:building an ecological approach to the synthesis of molecular and quantitative genetic methods［J］.Ecology of Freshwater Fish,2002,11(1):30-55.

8.张宏达,黄云峰,缪汝槐,等.种子植物系统［M］.北京:科学出版社,2004:319-320.

9.乔琪,邢福武,陈红峰,等.中国特有珍稀植物伯乐树的研究进展和科研方向［J］.中国野生植物资源,2011,30(3):4-8.

10.梁艳,徐刚标,张合平,等.南岭山地伯乐树天然种群和人工种群遗传多样性比较［J］.林业科学,2012,48(12):45-52.

11.徐刚标,梁艳,蒋燚,等.伯乐树种群遗传多样性及遗传结构［J］.生物多样性,2013,21(6):723-731.

12.王美娜,乔琪,张荣京,等.广东南昆山与大岭山孑遗植物伯乐树群落特征比较与谱系地理学研究［J］.广西植物,2011,31(6):789-794.

13.傅立国.中国植物红皮书［M］.北京:科学出版社,1992:194-195.

14.于永福.中国野生植物保护工作的里程碑（国家重点保护野生植物名录（第1批））［J］.植物杂志,1999(5):1-3.

15.Ronse De Craene L P,Yang T Y A,Schols P,et al.Floral anatomy and systematics of Bretschneidera(Bretschneideraceae)［J］.Bot J Linn Soc,2002,139(1):29-45.

16.王娟,刘仁林,廖为明.伯乐树生长发育节律和物候特征研究［J］.江西科学,2008,26(4):552-555.

17.Qiao Q,Chen H F,Xing F W,et al.Seed germination protocol for the threatened plant species,Bretschneidera sinensis Hemsl［J］.Seed Sci Tech,2009,37(1):70-78.

18.郭治友,龙应霞,肖国学.钟萼木的组织培养和快速繁殖［J］.植物生理学通迅,2007,43(1):127.

19.林国锋.濒危植物钟萼木的分子标记及遗传多样性研究［D］.福州:福建农林大学,2011.

20.Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］.Phytoch Bull,1987,19:11-15.

21.谢正成,楼雄珍,姚理武,等.伯乐树ISSR分子标记体系的建立［J］.浙江林业科技,2011,31(1):11-15.

22.Nei M.Analysis of gene diversity in subdivided populations［J］.Proc Natl Acad Sci,USA,1973,70:3321-3323.

23.Lewinton R C.The apportionment of human diversity［J］.Evol Biol,1972,6:381-398.

24.冷欣,王中生,安树青,等.岛屿特有种全缘冬青的ISSR分析［J］.生物多样性,2005,13(6):546-554.

25.Excoffier L,Smouse P E,Quattro J M.Analysis of molecular variance inferred from metric distances among DNA haplotypes:application to human mitochondrial DNA restriction data［J］.Genetics,1992,131:479-491.

26.Nybom H.Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants［J］.Mol Ecol,2004,13(5):1143-1155.

27.Cao P J,Yao Q F,Ding B Y,et al.Genetic diversity of Sinojackia dolichocarpa(Styracaceae),a species endangered and endemic to China,detected by inter-simple sequence repeat(ISSR)［J］.Biochem Syst Ecol,2006,34:231-239.

28.Zawko G,Krauss S L,Dixon K W,et al.Conservation genetics of the rare and endangered Leucopogon obtectus(Ericaceae)［J］.Mol Ecol,2001,10:2389-2396.

29.Qiao Q,Chen H F,Xing F W,et al.Pollination ecology of Bretschneidera sinensis(Hemsley),a rare and endangered tree in China［J］.Pakistan Journal Botany,2012,44:1897-1903.

30.Leberg P L.Genetic considerations in the design of introduction programs transactions of the north American Wildlife［J］.Natural Resource Conference,1990,55:609-619.

31.徐凤华,康明,黄宏文,等.濒危植物毛柄小勾儿茶片断化居群的遗传多样性［J］.植物生态学报,2006,30(1):157-164.

32.Stefenon V M,Gailing O,Finkeldey R.Genetic structure of plantations and the conservation of genetic resources of Brazilian pine(Araucaria angustifolia)［J］.For Ecol Manage,2008,255(7):2718-2725.

33.王蕾,施诗,廖文波,等.井冈山地区珍惜濒危植物及其生存状况［J］.生物多样性,2013,21(2):163-169.

[1]	杨磊, 曹秋梅, 冯缨, 李文军. 珍稀濒危植物阜康阿魏的遗传多样性及遗传结构[J]. 植物研究, 2023, 43(1): 51-58.
[2]	唐思如, 王铁娟, 秀芳, 刘梦瑶, 张慧. 基于单拷贝核基因的褐沙蒿遗传结构和谱系地理学分析[J]. 植物研究, 2022, 42(6): 956-963.
[3]	常二梅, 刘建锋, 黄跃宁, 李红丽, 单冰燕, 江泽平, 赵秀莲. 岷江柏野生居群和迁地保护居群的遗传多样性比较[J]. 植物研究, 2022, 42(5): 772-779.
[4]	张玉洁, 石鹏霞, 赵凡, 陈晓丹, 孙坤, 张辉. rpl32-trnL片段在柳穿鱼不同地理居群遗传多样性分析中的应用[J]. 植物研究, 2022, 42(5): 780-789.
[5]	张阳, 马子兰, 徐珊珊, 苏旭, 李梅英. 青藏高原东北部黄缨菊的谱系地理学[J]. 植物研究, 2022, 42(4): 565-573.
[6]	张宏斌, 吕东, 赵明, 赵祜, 赵兴鹏, 李伟. 基于全基因组SNP分子标记分析青海云杉遗传结构[J]. 植物研究, 2022, 42(3): 373-382.
[7]	张序, 张秀姣, 马永鹏, 李正红, 万友名, 马宏. 基于GBS简化基因组技术的宽杯杜鹃遗传多样性分析[J]. 植物研究, 2021, 41(3): 429-435.
[8]	段义忠, 王海涛, 张格格, 严伟娜. 基于ISSR分子标记的孑遗濒危植物四合木遗传结构分析[J]. 植物研究, 2021, 41(3): 466-473.
[9]	吕冬雪, 张学杰, 张洛艳, 樊守金. 基于SSR技术对黄河三角洲二色补血草遗传多样性研究[J]. 植物研究, 2020, 40(6): 923-931.
[10]	宁馨, 姜小龙, 邓敏, 徐刚标. 基于简化基因组数据开发岭南青冈微卫星引物[J]. 植物研究, 2020, 40(4): 629-634.
[11]	管睿婷, 刘济明, 王敏, 陈敬忠, 武梦瑶, 童炳丽. 不同天然居群小蓬竹的遗传多样性[J]. 植物研究, 2019, 39(5): 740-751.
[12]	郭文丽, 李义良, 赵奋成, 铁军, 廖仿炎, 钟岁英, 林昌明, 叶威方. 湿加松无性系表型遗传多样性研究[J]. 植物研究, 2019, 39(2): 259-266.
[13]	刘济明, 管睿婷, 王敏, 陈敬忠, 童炳丽, 武梦瑶. 不同喀斯特小生境中小蓬竹的遗传多样性[J]. 植物研究, 2019, 39(1): 69-77.
[14]	蔡年辉, 许玉兰, 王亚楠, 陈诗, 汪梦婷, 李根前. 基于SSR标记的不同优势等级云南松遗传多样性分析[J]. 植物研究, 2019, 39(1): 87-95.
[15]	肖遥, 麻文俊, 易飞, 杨桂娟, 王秋霞, 王平, 王军辉. 滇楸种质生长性状遗传变异及表型性状遗传多样性分析[J]. 植物研究, 2018, 38(6): 843-851.